A5091159789- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Adenosine and Purinergic Signaling
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Signaling Pathways in Disease
- Hematopoietic Stem Cell Transplantation
- Psoriasis: Treatment and Pathogenesis
- GDF15 and Related Biomarkers
- MicroRNA in disease regulation
- Cancer therapeutics and mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Neutropenia and Cancer Infections
- Colorectal Cancer Treatments and Studies
- Ubiquitin and proteasome pathways
- Acute Myeloid Leukemia Research
Université de Montpellier
2016-2025
Institut de Recherche en Cancérologie de Montpellier
2016-2025
Immunité et Cancer
2025
Inserm
2015-2024
CEA Valduc
2014-2023
Institut Regional du Cancer de Montpellier
2013-2021
Centre National de la Recherche Scientifique
1989-2019
OREGA Biotech (France)
2013-2018
RELX Group (United States)
2018
Centre de Génétique Moléculaire
1994-2017
Immune checkpoint inhibitors have revolutionized cancer treatment. However, many cancers are resistant to ICIs, and the targeting of additional inhibitory signals is crucial for limiting tumor evasion. The production adenosine via sequential activity CD39 CD73 ectoenzymes participates generation an immunosuppressive microenvironment. In order disrupt pathway, we generated two antibodies, IPH5201 IPH5301, human membrane-associated soluble forms CD73, respectively, efficiently blocking...
Abstract Host immunity controls the development of colorectal cancer, and chemotherapy used to treat cancer is likely recruit host immune system at some level. Athough preclinical studies have argued that drugs, such as 5-fluorouracil (5-FU) oxaliplatin, exert effects, their combination employed in oncology clinic has not been evaluated. Here, we report results prospective immunomonitoring 25 metastatic (mCRC) patients treated with a first-line regimen 5-FU, bevacizumab (FOLFOX–bevacizumab),...
The ectonucleotidases CD39 and CD73 hydrolyze extracellular adenosine triphosphate (ATP) diphosphate (ADP) to generate adenosine, which binds receptors inhibits T-cell natural killer (NK)-cell responses, thereby suppressing the immune system. generation of via CD39/CD73 pathway is recognized as a major mechanism regulatory T cell (Treg) immunosuppressive function. number CD39⁺ Tregs increased in some human cancers, importance promoting tumor growth metastasis has been demonstrated using...
Antilymphocyte or antithymocyte globulins were shown to be immunosuppressive when administered recipients of organ transplants as prophylactic rescue treatment acute rejection in patients with graft versus host reactions following bone marrow transplantation. Several monoclonal antibodies specific for activation adhesion molecules the T lymphocyte surface can also inhibit experimental clinical allograft reactions. We have investigated presence some antibodies, defined specificity and...
TLR have a crucial role in the detection of microbial infection mammals. Until recently, most investigations on focused cells innate immune system and initiation antigen-specific responses following recognition products by APC. Here, we report that murine T express TLR1, TLR2, TLR6, TLR7 TLR9 mRNA. Using CD8 from F5 TCR-transgenic mice, demonstrate lipopeptide Pam(3)CysSK(4) (Pam), synthetic analog bacterial mycoplasmal lipoproteins recognizes TLR1/2 complex, costimulates antigen-activated...
Objective Systemic lupus erythematosus (SLE) is a prototype autoimmune disease that assumed to occur via complex interplay of environmental and genetic factors. Rare causes monogenic SLE have been described, providing unique insights into fundamental mechanisms immune tolerance. The aim this study was identify the cause an autosomal‐recessive form SLE. Methods We studied 3 siblings with juvenile‐onset from 1 consanguineous kindred used next‐generation sequencing mutations in...
The proinflammatory cytokine Interleukin 17A (hereafter named IL–17A) or IL-17A producing cells are elevated in breast tumors environment and correlate with poor prognosis. Increased is associated ER(−) triple negative reduced Disease Free Survival. However, the pathophysiological role of cancer remains unclear although several studies suggested its involvement cell dissemination. Here we demonstrated that a subset infiltrated IL-17A-producing cells. seems mainly to negative/basal-like...
Recent studies have documented direct interactions between 14-3-3 proteins and several oncogene proto-oncogene products involved in signal transduction pathways. Studies on the effects of protein kinase C (PKC) activity vitro reported conflicting results, previous attempts to demonstrate a association PKC were unsuccessful. Here, we examined potential physical functional theta, Ca(2+)-independent enzyme which is expressed selectively T lymphocytes, tau isoform intact cells. theta...
Proteins of the 14-3-3 family can associate with, and/or modulate activity of, several protooncogene and oncogene products and, thus, are implicated in regulation signaling pathways. We report that is associated with another important transducing enzyme, phosphatidylinositol 3-kinase (PI3-K). A recombinant fusion protein bound tyrosine-phosphorylated proteins from antigen receptor-stimulated T lymphocytes. PI3-K was identified by immunoblotting enzymatic assays as one 14-3-3-binding resting...
We recently identified Vav as a Ras-activating guanine nucleotide exchange factor (GEF) stimulated by T-cell antigen receptor-coupled protein tyrosine kinase (PTK). Here, we describe novel, kinase-independent alternative pathway of activation. Phorbol ester, 1,2-diacylglycerol, or ceramide treatment intact T cells, immunoprecipitates, partially purified generated in vitro translation COS-1 cell transfection the Ras activity absence detectable phosphorylation. GEF gel-purified was similarly...
TLR are involved in the detection of microbial infection as well endogenous ligands that signal tissue and cell damage mammals. This recognition plays an essential role innate immune response initiation adaptive response. We have previously shown murine CD8 T cells express TLR2, costimulation Ag-activated with TLR2 enhances their proliferation, survival, effector functions. also demonstrated engagement on significantly reduces need for costimulatory signals delivered by APC. show this study...
Abstract Cross-talk between NK cells and dendritic (DCs) is critical for the potent therapeutic response to dsRNA, but receptors involved remained controversial. We show in this paper that two dsRNAs, polyadenylic-polyuridylic acid polyinosinic-polycytidylic [poly(I:C)], similarly engaged human TLR3, whereas only poly(I:C) triggered RIG-I MDA5. Both dsRNA enhanced cell activation within PBMCs induced IFN-γ. Although myeloid DCs (mDCs) were required activation, induction of cytolytic...
Triple-negative breast cancer (TNBC) treatment is currently restricted to chemotherapy. Hence, tumor-specific molecular targets and/or alternative therapeutic strategies for TNBC are urgently needed. Immunotherapy emerging as an exciting option patients. The aspartic protease cathepsin D (cath-D), a marker of poor prognosis in (BC), overproduced and hypersecreted by human BC cells. This study explores whether cath-D tumor cell-associated extracellular biomarker potent target antibody-based...
Although many patients with colorectal cancer initially respond to the chemotherapeutic agent oxaliplatin, acquired resistance this treatment remains a major challenge long-term management of disease. To identify molecular targets oxaliplatin in cancer, we performed an shRNA-based loss-of-function genetic screen using kinome library. We found that silencing ataxia-telangiectasia mutated and RAD3-related (ATR), serine/threonine protein kinase involved response DNA stress, restored sensitivity...
Relapses driven by chemoresistant leukemic cell populations are the main cause of mortality for patients with acute myeloid leukemia (AML). Here, we show that ectonucleotidase CD39 (ENTPD1) is upregulated in cytarabine-resistant cells from both AML lines and patient samples vivo vitro. cell-surface expression activity increased upon chemotherapy compared diagnosis, enrichment CD39-expressing blasts a marker adverse prognosis clinics. High promotes cytarabine resistance enhancing...
14-3-3 proteins have recently been implicated in the regulation of intracellular signaling pathways via their interaction with several oncogene and protooncogene products. We found that associates tyrosine-phosphorylated phosphatidylinositol 3-kinase (PI3-K) T cells. report here identification 120-kDa 14-3-3tau-binding phosphoprotein present activated cell lysates as Cbl, a product unknown function which was to be major protein-tyrosine kinase (PTK) substrate, interact molecules including...
The yeast nuclear gene OXA1 is essential for cytochrome oxidase assembly, so that a null mutation in the leads to complete respiratory deficiency. We have cloned by genetic selection human (OXA1Hs) cDNA complements defect of oxa1 mutants. deduced sequence protein shares 33% identity with protein. OXA1Hs corresponds single and relatively highly expressed gene. Oxygen consumption measurements absorption spectra show replacement homolog correct assembly oxidase, suggesting proteins play...