A5012204939- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Genetic diversity and population structure
- Forest Insect Ecology and Management
- Botanical Studies and Applications
- Cell Adhesion Molecules Research
- Plant and animal studies
- HIV Research and Treatment
- Adenosine and Purinergic Signaling
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Atherosclerosis and Cardiovascular Diseases
- HER2/EGFR in Cancer Research
- Plant Reproductive Biology
- Plant Ecology and Taxonomy Studies
- Identification and Quantification in Food
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Systemic Lupus Erythematosus Research
- Plant Physiology and Cultivation Studies
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Horticultural and Viticultural Research
Institut de Recherche en Cancérologie de Montpellier
2014-2025
Immunité et Cancer
2014-2025
Université de Montpellier
2010-2025
Inserm
2014-2024
Institut Regional du Cancer de Montpellier
2017-2020
Centre d'Écologie Fonctionnelle et Évolutive
1992-2019
Centre National de la Recherche Scientifique
1992-2019
Gefluc Languedoc Roussillon
2014
University of California, San Francisco
2012-2014
Institut de Génétique Moléculaire de Montpellier
2007-2010
Immune checkpoint inhibitors have revolutionized cancer treatment. However, many cancers are resistant to ICIs, and the targeting of additional inhibitory signals is crucial for limiting tumor evasion. The production adenosine via sequential activity CD39 CD73 ectoenzymes participates generation an immunosuppressive microenvironment. In order disrupt pathway, we generated two antibodies, IPH5201 IPH5301, human membrane-associated soluble forms CD73, respectively, efficiently blocking...
Abstract Variation in the lengths of restriction fragments (RFLPs) whole chloroplast DNA molecule was studied 174 populations Quercus ilex L. sampled over entire distribution this evergreen and mainly Mediterranean oak species. By using five endonucleases, 323 distinct were obtained. From 29 17 cpDNA changes identified as site length mutations, respectively, 25 chlorotypes distinguished, mapped treated cladistically with a parsimony analysis, an outgroup Q. alnifolia Poech, closely related...
Triple-negative breast cancer (TNBC) treatment is currently restricted to chemotherapy. Hence, tumor-specific molecular targets and/or alternative therapeutic strategies for TNBC are urgently needed. Immunotherapy emerging as an exciting option patients. The aspartic protease cathepsin D (cath-D), a marker of poor prognosis in (BC), overproduced and hypersecreted by human BC cells. This study explores whether cath-D tumor cell-associated extracellular biomarker potent target antibody-based...
Although many patients with colorectal cancer initially respond to the chemotherapeutic agent oxaliplatin, acquired resistance this treatment remains a major challenge long-term management of disease. To identify molecular targets oxaliplatin in cancer, we performed an shRNA-based loss-of-function genetic screen using kinome library. We found that silencing ataxia-telangiectasia mutated and RAD3-related (ATR), serine/threonine protein kinase involved response DNA stress, restored sensitivity...
In this paper we review the characteristics of floral biology, life cycle and breeding system in genus Quercus.The species are self-incompatible have very long spans.The focus our is on effects gene flow structuration genetic varia- tion these species.We examined influence 2 ways: 1) by measuring physical dispersal pollen, seed vegetative organs; 2) using nuclear cytoplasmic markers to estimate parameters (F , N m ).These approaches shown that (iso- zyme markers) as well (chloroplastic DNA)...
Abstract Pyruvate metabolism defects lead to severe neuropathies such as the Leigh syndrome (LS) but molecular mechanisms underlying neuronal cell death remain poorly understood. Here, we unravel a connection between pyruvate and regulation of epitranscriptome that plays an essential role during brain development. Using genetically engineered mouse model primary cells, identify transcription factor E4F1 key coordinator AcetylCoenzyme A (AcCoA) production by dehydrogenase complex (PDC) its...
Background Colorectal cancer (CRC) is the third most common type and one of leading causes cancer-related death worldwide. The treatment advanced metastatic CRC relies on classical chemotherapy combinations (5-fluorouracil, oxaliplatin or irinotecan). However, their use limited by emergence resistance mechanisms, including to oxaliplatin. In this context, we recently showed that combination ataxia telangiectasia Rad3-related protein inhibition (VE-822) synergistic may have a potential...
Antiviral monoclonal antibodies (mAbs) represent promising therapeutics. However, most mAbs-based immunotherapies conducted so far have only considered the blunting of viral propagation and not other possible therapeutic effects independent virus neutralization, namely modulation endogenous immune response. As induction long-term antiviral immunity still remains a paramount challenge for treating chronic infections, we asked here whether neutralizing mAbs can, in addition to propagation,...
Abstract Background Human Endogenous Retroviruses (HERVs) comprise about 8% of the human genome and have lost their ability to replicate or produce infectious particles after having accumulated mutations over time. We assessed kinetics expression HERV-K (HML-2) Envelope mRNA transcript surface unit (SU) transmembrane (TM) subunit proteins during HIV-1 infection. also mapped specificity humoral response protein in infected subjects at different stages disease, correlated with plasma viral...
// Emilie Laprevotte 1 , Stéphanie Cochaud Stanislas du Manoir 2 Marion Lapierre Cécile Dejou Philippe Jérome Giustiniani 3, 4 Kathryn A. Frewer 5 Andrew J. Sanders Wen G. Jiang Henri-Alexandre Michaud Pierre-Emmanuel Colombo 6 Armand Bensussan 3 Gilles Alberici Jérémy Bastid Jean-François Eliaou 2, 7 and Nathalie Bonnefoy OREGA Biotech, Ecully, F-69130 France IRCM, INSERM, Université de Montpellier, ICM, F-34298 Institut National la Santé et Recherche Médicale (INSERM) UMR-S 976, Paris...
Abstract γδ T cells contribute to the immune response against many cancers, notably through their powerful effector functions that lead elimination of tumor and recruitment other cells. However, presence in microenvironment has been associated with poor prognosis breast, colon, pancreatic cancer, suggesting may also display pro-tumor activities. Here, we identified blood from healthy donors a subpopulation Vδ1T represents around 20% whole Vδ1 population, expresses CD73, displays...
Macrophages play a central role in tissue homeostasis and host defense. However, the properties of human macrophages non-diseased tissues remain poorly understood. Here, we characterized tonsil identified three subsets with distinct phenotype, transcriptome, life cycle, function. CD36hi were related to monocytes, while CD36lo showed features embryonic origin CD36int had mixed profile. scRNA-seq on non-human primate tonsils that monocyte recruitment did not pre-exist an immune challenge....
Dendritic cells (DCs) play a key role in the induction of adaptive and memory immune responses. Upon encounter with pathogens, they undergo complex maturation process migrate toward lymphoid organs where stimulate effector cells. This is associated dramatic transcriptome changes, pointing to paramount for transcription factors DC activation function. The regulation these are however ill-defined require characterization. Among those, AP-1 family dimeric complexes an acknowledged control...
Vγ9Vδ2 T cells contribute to the immune response against many tumor types through their direct cytotoxic activity and capacity regulate biological functions of other cells, such as dendritic IFN-γ-producing CD8+ cells. However, presence in microenvironment has also been associated with poor prognosis breast, colon pancreatic cancers. Additionally, recent studies demonstrated that cytokines can confer some plasticity promote differentiation into regulatory functions. Here, we activation...
Human endogenous retroviruses (HERVs) comprise approximately 8% of the human genome and while majority are transcriptionally silent, most recently integrated HERV, HERV-K (HML-2), remains active. During HIV infection, (HML-2) specific mRNA transcripts viral proteins can be detected. In this study, we aimed to understand antibody response against Gag in context HIV-1 infection. We developed an ELISA assay using either recombinant protein or 164 redundant "15mer" peptides test sera for...
Low-coverage whole genome shotgun sequencing (or skimming) has emerged as a cost-effective method for acquiring genomic data in nonmodel organisms. This provides sequence information on chloroplast (cpDNA), mitochondrial (mtDNA) and nuclear ribosomal regions (rDNA), which are over-represented within cells. However, numerous bioinformatic challenges remain to accurately rapidly obtain such organisms with complex structures rearrangements, particular mtDNA plants or cpDNA some plant families....
ABSTRACT Using FrCas E retrovirus-infected newborn mice as a model system, we have shown recently that long-lasting antiviral immune response essential for healthy survival emerges after short treatment with neutralizing (667) IgG2a isotype monoclonal antibody (MAb). This suggested the mobilization of adaptive immunity by administered MAbs is key success in long term MAb-based passive immunotherapy chronic viral infections. We addressed here whether anti-FrCas protective endogenous mere...
Abstract The failure of antiviral vaccines is often associated with rapid viral escape from specific immune responses. In the past, conserved epitope or algorithmic selections, such as mosaic vaccines, have been designed to diversify immunity and circumvent potential escape. An alternative approach identify stable non–HIV-1 self-epitopes present exclusively in HIV-1–infected cells. We showed previously that human endogenous retroviral (HERV) mRNA transcripts protein are found cells patients...