A5066278788- Environmental DNA in Biodiversity Studies
- Geology and Paleoclimatology Research
- Genetic diversity and population structure
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Isotope Analysis in Ecology
- Forest Ecology and Biodiversity Studies
- Climate change and permafrost
- Identification and Quantification in Food
- Protist diversity and phylogeny
- Species Distribution and Climate Change
- Indigenous Studies and Ecology
- Plant and animal studies
- Insect behavior and control techniques
- Bat Biology and Ecology Studies
- Mollusks and Parasites Studies
- Animal Ecology and Behavior Studies
- Biological Control of Invasive Species
- Astrophysics and Cosmic Phenomena
- Forest Insect Ecology and Management
- Peatlands and Wetlands Ecology
- Forensic and Genetic Research
- Archaeology and ancient environmental studies
- Plant and Fungal Species Descriptions
- Amphibian and Reptile Biology
UiT The Arctic University of Norway
2018-2025
Tromsø research foundation
2023
Naturalis Biodiversity Center
2012-2023
ORCID
2021
Centre for Arctic Gas Hydrate, Environment and Climate
2020
University of Applied Sciences Leiden
2016
Leiden University
2011-2016
Leiden University Medical Center
2013
The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field paleoecological research. Although largely applied nowadays, knowledge gaps remain this there therefore still research be conducted ensure reliability signal. Building on most recent literature seven original case studies, we synthesize state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation...
Metabarcoding of lake sediments have been shown to reveal current and past biodiversity, but little is known about the degree which taxa growing in vegetation are represented environmental DNA (eDNA) records. We analysed composition catchment vascular plant eDNA at 11 lakes northern Norway. Out 489 records within 2 m from shore, 17–49% (mean 31%) identifiable recorded were detected with eDNA. Of 217 47 lakes, 73% 12% matched surveys up 50 away lakeshore, respectively, whereas 16% not same...
Abstract During the last glacial–interglacial cycle, Arctic biotas experienced substantial climatic changes, yet nature, extent and rate of their responses are not fully understood 1–8 . Here we report a large-scale environmental DNA metagenomic study ancient plant mammal communities, analysing 535 permafrost lake sediment samples from across spanning past 50,000 years. Furthermore, present 1,541 contemporary genome assemblies that were generated as reference sequences. Our provides several...
Invasive individuals from the pest species Jacobaea vulgaris show different allocation patterns in defence and growth compared with native individuals. To examine if these changes are caused by fast evolution, it is necessary to identify source populations compare invasive populations. For this purpose, we need of intraspecific polymorphic markers. We therefore sequenced complete chloroplast genomes 12 5 J. next generation sequencing discovered single-nucleotide polymorphisms (SNPs)...
The lake sediments of Hässeldala Port in south-east Sweden provide an archive local and regional environmental conditions ~14.5-9.5 ka BP (thousand years before present) allow testing DNA sequencing techniques to reconstruct past vegetation changes. We combined shotgun with plant micro- macrofossil analyses investigate dating the Allerød (14.1-12.7 BP), Younger Dryas (12.7-11.7 BP) Preboreal (<11.7 BP). Number reads taxa were not associated sample age or organic content. This suggests that,...
Genome skimming has the potential for generating large data sets DNA barcoding and wider biodiversity genomic studies, particularly via assembly annotation of full chloroplast (cpDNA) nuclear ribosomal (nrDNA) sequences. We compare success genome skims 2051 herbarium specimens from Norway/Polar regions with 4604 freshly collected, silica gel dried mainly European Alps Carpathians. Overall, we were able to assemble 67% samples nrDNA cluster 86%. Average insert length, cover cpDNA rDNA...
Abstract The European Alps are highly rich in species, but their future may be threatened by ongoing changes human land use and climate. Here, we reconstructed vegetation, temperature, impact livestock over the past ~12,000 years from Lake Sulsseewli, based on sedimentary ancient plant mammal DNA, pollen, spores, chironomids, microcharcoal. We assembled a highly-complete local DNA reference library (PhyloAlps, 3923 taxa), used this to obtain an exceptionally sed aDNA record of 366 taxa....
What drives ecosystem buildup, diversity, and stability? We assess species arrival changes across 16 millennia by combining regional-scale plant sedimentary ancient DNA from Fennoscandia with near-complete trait databases. show that postglacial time varies within between growth forms. Further, times were mainly predicted adaptation to temperature, disturbance, light. Major break points in ecological diversity seen 13.9 10.8 calibrated thousand years before the present (cal ka BP), as well...
Western European coastal sand dunes are highly important for nature conservation. Communities of the creeping willow (Salix repens) represent one most characteristic and diverse vegetation types in dunes. We report here results first kingdom-wide fungal diversity assessment S. repens dune vegetation. carried out massively parallel pyrosequencing ITS rDNA from soil samples taken at ten sites an extended area joined reserves located along North Sea coast Netherlands, representing habitats with...
Understanding patterns of colonisation is important for explaining both the distribution single species and anticipating how ecosystems may respond to global warming. Insular flora be especially vulnerable because oceans represent severe dispersal barriers. Here we analyse two lake sediment cores from Iceland ancient sedimentary DNA infer Holocene vegetation development. Our lakes Torfdalsvatn Nykurvatn span last c. 12,000 cal yr BP 8600 BP, respectively. With near-centennial resolution,...
SedaDNA reveals regional climate and bedrock nutrients as major drivers of terrestrial plant diversity in northern Fennoscandia.
Understanding the molecular basis of domestication can provide insights into processes rapid evolution and crop improvement. Here we demonstrated carrot identified genes under selection based on transcriptome analyses. The root transcriptomes widely differing cultivated wild carrots were sequenced. A method accounting for sequencing errors was introduced to optimize SNP (single nucleotide polymorphism) discovery. 11,369 SNPs identified. Of these, 622 (out 1000 tested SNPs) validated used...
Andøya on the NW coast of Norway is a key site for understanding Last Glacial Maximum (LGM) in northern Europe. Controversy has arisen concerning local conditions, especially about timing and extent glacial cover, maximum July temperatures whether pine and/or spruce could have grown there. We reviewed all existing data add newly analysed ancient sedimentary DNA (sedaDNA), pollen, macrofossils, geochemistry stable isotopes from three lake sediment cores Øvre Æråsvatnet. A total 23 new dates...
<title>Abstract</title> We present a decade-long research initiative of biodiversity genomics, which aimed at sequencing all vascular plant species occurring in the hotspot European Alps, along with related lineages other mountain ranges and arctic region. detail sampling strategies, an online database for genome skimming datasets, bioinformatic procedures to retrieve sequence data. Based on low coverage shotgun 6,105 species, we generated dataset 81 orthologous chloroplast loci, including...
Abstract Next‐generation sequencing is a fast and cost‐effective way to obtain sequence data for nonmodel organisms many markers individuals. We describe protocol through which we orthologous the crested newts (Amphibia: Salamandridae: Triturus ), suitable analysis of interspecific hybridization. use transcriptome single species design 96 primer pairs that amplify c . 180 bp fragments positioned in 3‐prime untranslated regions. Next, these are tested with uniplex PCR set spanning taxonomical...
We present a Holocene record of floristic diversity and environmental change for the central Varanger Peninsula, Finnmark, based on ancient DNA extracted from sediments small lake ( sed ). The covers period c . 10 700 to 3300 cal. BP is complemented by pollen data. Measures species richness, sample evenness beta were calculated sampling intervals 1000‐year time windows. identified 101 vascular plant 17 bryophyte taxa, high proportion (86%) which are still growing within region today....
Metagenomics can generate data on the diet of herbivores, without need for primer selection and PCR enrichment steps as is necessary in metabarcoding. Metagenomic approaches to analysis have remained relatively unexplored, requiring validation bioinformatic steps. Currently, no metagenomic herbivore studies utilized both chloroplast nuclear markers reference sequences plant identification, which would increase number reads that could be taxonomically informative. Here, we explore how silico...
The integration of invasive species into native food webs represent multifarious dynamics ecological and evolutionary processes. We document incorporation Prunus serotina (black cherry) insect webs. find that P. harbours a herbivore community less dense but more diverse than its relative, padus (bird cherry), with similar proportions specialists generalists. While herbivory on remained stable over the past century, gradually doubled. show may have evolved changes in investment cyanogenic...
Population size has increasingly been taken as the driver of past human environmental impact worldwide, and particularly in Arctic. However, sedimentary ancient DNA (sedaDNA), pollen archaeological data show that over last 12,000 years, paleoeconomy culture determined impacts on terrestrial ecology Arctic Norway. The large Mortensnes site complex (Ceavccageađgi, 70°N) yielded most comprehensive multiproxy record to date. saw occupation from Pioneer period (c. 10,000 cal. years BP) with more...
Both climate change and anthropogenic disturbance affect vegetation composition, but it is difficult to separate these drivers of from one another. A better understanding past dynamics necessary disentangle the influence different forcing factors assess future change. Here we present first multi-proxy palaeoecological study combining sedimentary ancient DNA (sedaDNA), pollen, spores, stomata, charcoal plant macrofossils Alps. We reconstructed Holocene fire history at Lago Inferiore del...
Low-coverage whole genome shotgun sequencing (or skimming) has emerged as a cost-effective method for acquiring genomic data in nonmodel organisms. This provides sequence information on chloroplast (cpDNA), mitochondrial (mtDNA) and nuclear ribosomal regions (rDNA), which are over-represented within cells. However, numerous bioinformatic challenges remain to accurately rapidly obtain such organisms with complex structures rearrangements, particular mtDNA plants or cpDNA some plant families....
While there are extensive macro‐ and microfossil records of a range plants animals from the Quaternary, earthworms their close relatives amongst annelids not preserved as fossils therefore knowledge past distributions is limited. This lack means that clitellate worms (Annelida) currently underused in palaeoecological research, even though they can provide valuable information about terrestrial aquatic environmental conditions. Their DNA might be sediments, which offers an alternative method...
Mixtures of internationally traded organic substances can contain parts species protected by the Convention on International Trade in Endangered Species Wild Fauna and Flora (CITES). These mixtures often raise suspicion border control customs offices, which lead to confiscation, for example case Traditional Chinese medicines (TCMs). High-throughput sequencing DNA barcoding markers obtained from such samples provides insight into constituents mixtures, but manual cross-referencing results...