A5033279421- Environmental DNA in Biodiversity Studies
- Microbial Community Ecology and Physiology
- Identification and Quantification in Food
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Marine and coastal plant biology
- Marine Bivalve and Aquaculture Studies
- Species Distribution and Climate Change
- Coral and Marine Ecosystems Studies
- Marine Ecology and Invasive Species
- Ocean Acidification Effects and Responses
- Isotope Analysis in Ecology
- Genetic diversity and population structure
- Marine and fisheries research
- Fish Ecology and Management Studies
- Cephalopods and Marine Biology
- Echinoderm biology and ecology
- Bat Biology and Ecology Studies
- Biomedical Text Mining and Ontologies
- Photosynthetic Processes and Mechanisms
- Scientific Computing and Data Management
- Animal Ecology and Behavior Studies
- Rangeland and Wildlife Management
- Animal Vocal Communication and Behavior
Universitat de Barcelona
2011-2025
Institut de Biologia Evolutiva
2022-2025
Universitat Autònoma de Barcelona
2024
UiT The Arctic University of Norway
2018-2024
Norwegian Institute of Marine Research
2023
University of Salford
2016-2020
Centre d'Estudis Avançats de Blanes
2015-2018
Vall d'Hebron Hospital Universitari
2010
Estación Experimental del Zaidín
1998-2001
As environmental DNA (eDNA) becomes an increasingly valuable resource for marine ecosystem monitoring, understanding variation in its persistence across contrasting environments is critical. Here, we quantify the breakdown of macrobial eDNA over a spatio-temporal axis locally extreme conditions, varying from ocean-influenced offshore to urban-inshore, and between winter summer. We report that degrades 1.6 times faster inshore environment than environment, but contrary expectation find no...
Abstract Metabarcoding extra‐organismal DNA from environmental samples is now a key technique in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when designing experiments, especially so developing community standards legislative frameworks, the choice of genetic marker primer set. Mitochondrial cytochrome c oxidase subunit I (COI), standard barcode for animals, with its extensive reference library, taxonomic discriminatory power predictable sequence...
Environmental DNA reveals unsuspected shark diversity and calls for monitoring protection of residual populations.
Biodiversity assessment of marine hard-bottom communities is hindered by the high diversity and size-ranges organisms present. We developed a DNA metabarcoding protocol for biodiversity characterization structurally complex natural communities. used two molecular markers: “Leray fragment” mitochondrial cytochrome c oxidase (COI), which novel primer set was developed, V7 region nuclear small subunit ribosomal RNA (18S). Eight different shallow littoral from National Parks in Spain (one...
Marine sediments are home to one of the richest species pools on Earth, but logistics and a dearth taxonomic work-force hinders knowledge their biodiversity. We characterized α- β-diversity deep-sea assemblages from submarine canyons in western Mediterranean using an environmental DNA metabarcoding. used new primer set targeting short eukaryotic 18S sequence (ca. 110 bp). applied protocol designed obtain extractions enriched extracellular replicated sediment corers. With this strategy we...
The recent blooming of metabarcoding applications to biodiversity studies comes with some relevant methodological debates. One such issue concerns the treatment reads by denoising or clustering methods, which have been wrongly presented as alternatives. It has also suggested that denoised sequence variants should replace clusters basic unit analyses, missing fact are a proxy for species-level entities, in studies. We argue here methods developed and tested ribosomal markers uncritically...
The latest animal phylum to be discovered, Micrognathozoa, constitutes a rare group of limnic meiofauna. These microscopic 'jaw animals' are among the smallest metazoans yet possess highly complex jaw structures. single species Limnognathia maerski Kristensen and Funch, 2000, was first described from Greenland, later reported remote Subantarctic island more recently discovered in Pyrenees on European continent. Successful collections these three known populations facilitated investigations...
Abstract Sharks are charismatic predators that play a key role in most marine food webs. Their demonstrated vulnerability to exploitation has recently turned them into flagship species ocean conservation. Yet, the assessment and monitoring of distribution abundance such mobile environments remain challenging, often invasive resource-intensive. Here we pilot novel, rapid non-invasive environmental DNA (eDNA) metabarcoding approach specifically targeted infer shark presence, diversity eDNA...
We assessed spatio-temporal patterns of diversity in deep-sea sediment communities using metabarcoding. chose a recently developed eukaryotic marker based on the v7 region 18S rRNA gene. Our study was performed submarine canyon and its adjacent slope Northwestern Mediterranean Sea, sampled along depth gradient at two different seasons. found total 5,569 molecular operational taxonomic units (MOTUs), dominated by Metazoa, Alveolata Rhizaria. Among metazoans, Nematoda, Arthropoda Annelida were...
Abstract Environmental DNA (eDNA) metabarcoding has revolutionized biomonitoring in both marine and freshwater ecosystems. However, for semi‐aquatic terrestrial animals, the application of this technique remains relatively untested. We first assess efficiency eDNA detecting mammals natural lotic ecosystems UK by comparing sequence data recovered from water sediment samples to mammalian communities expected historical data. Secondly, using occupancy modelling we compared detection multiple...
Abstract Metabarcoding is by now a well‐established method for biodiversity assessment in terrestrial, freshwater, and marine environments. data sets are usually used α‐ β‐diversity estimates, that is, interspecies (or inter‐ MOTU [molecular operational taxonomic unit] ) patterns. However, the use of hypervariable metabarcoding markers may provide an enormous amount intraspecies (intra‐ information—mostly untapped so far. The cytochrome oxidase ( COI amplicons gaining momentum studies...
In the marine realm, biomonitoring using environmental DNA (eDNA) of benthic communities requires destructive direct sampling or setting-up settlement structures. Comparatively much less effort is required to sample water column, which can be accessed remotely. this study we assess feasibility obtaining information from eukaryotic by adjacent layer. We studied two different rocky-substrate with a technique based on quadrat sampling. also took replicate samples at four distances (0, 0.5, 1.5,...
Abstract The European Alps are highly rich in species, but their future may be threatened by ongoing changes human land use and climate. Here, we reconstructed vegetation, temperature, impact livestock over the past ~12,000 years from Lake Sulsseewli, based on sedimentary ancient plant mammal DNA, pollen, spores, chironomids, microcharcoal. We assembled a highly-complete local DNA reference library (PhyloAlps, 3923 taxa), used this to obtain an exceptionally sed aDNA record of 366 taxa....
Introduction Currently, Arctic marine ecosystems are witnessing the most rapid physical changes worldwide, leading to shifts in pelagic and benthic communities food web structure, concomitant with introduction of boreal species. Gelatinous zooplankton or jellyfish represent one particular group which several species prone undergo significant poleward range expansions population increases course ongoing changes. Historically, were considered a trophic dead-end, but an increasing number...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 441:117-128 (2011) - DOI: https://doi.org/10.3354/meps09359 A wolf in sheep's clothing: carnivory dominant sea urchins Mediterranean Owen S. Wangensteen1,*, Xavier Turon2, Alex García-Cisneros1, Mireia Recasens1, Javier Romero3, Creu Palacín1 1Department of Animal Biology, University Barcelona, 08028 Spain...
Abstract Given their positioning and biological productivity, estuaries have long represented key providers of ecosystem services consequently remain under remarkable pressure from numerous forms anthropogenic impact. The monitoring fish communities in space time is one the most widespread established approaches to assess ecological status other coastal habitats, but traditional surveys are invasive, costly, labour intensive highly selective. Recently, application metabarcoding techniques,...
Abstract Environmental DNA (eDNA) has rapidly emerged as a promising biodiversity monitoring technique, proving to be sensitive and cost‐effective method for species detection. Despite the increasing popularity of eDNA, several questions regarding its limitations remain addressed. We investigated effect sampling medium time, preservation methods, on fish detection performance based eDNA metabarcoding neotropical freshwater samples. Water sediment samples were collected from 11 sites along...
Abstract Our understanding of marine communities and their functions in an ecosystem relies on the ability to detect monitor species distributions abundances. Currently, use environmental DNA (eDNA) metabarcoding is increasingly being applied for rapid assessment monitoring aquatic species. Most eDNA studies have either focussed simultaneous identification a few specific taxa/groups or been limited geographical scope. Here, we employed compare beta diversity patterns complex pelagic tropical...
Molecular gut-content analysis has revolutionized the study of food webs and feeding interactions, allowing detection prey DNA within gut many organisms. However, successful is a challenging procedure in which factors affect every step, starting from extraction process. Spiders are liquid feeders with branched diverticula extending into their legs throughout prosoma, thus digestion takes places different parts body simple dissection not possible. In this study, we investigated differences...
Abstract We focus on a case study along an English canal comparing environmental DNA (eDNA) metabarcoding with two types of electrofishing techniques (wade‐and‐reach and boom‐boat). In addition to corroborating data obtained by electrofishing, eDNA provided wider snapshot fish assemblages. Given the semi‐lotic nature canals, we encourage use as fast cost‐effective tool detect monitor whole communities.
Abstract Although metabarcoding is a well-established tool for describing diversity of pelagic communities, its quantitative value still controversial, with poor correlations previously reported between organism abundance/biomass and sequence reads. In this study, we explored an enhanced approach by whole zooplankton communities using highly degenerate primer set the mitochondrial marker cytochrome oxidase I compared results to biomass estimates obtained traditional morphological processing...