A5055063060- Botany and Plant Ecology Studies
- Genetic diversity and population structure
- Mycorrhizal Fungi and Plant Interactions
- Lichen and fungal ecology
- Plant Taxonomy and Phylogenetics
- Plant Diversity and Evolution
- Plant Pathogens and Fungal Diseases
- Chromosomal and Genetic Variations
- Plant and animal studies
- Plant and Fungal Species Descriptions
- Botanical Studies and Applications
- Botany, Ecology, and Taxonomy Studies
- Bryophyte Studies and Records
- Botanical Research and Chemistry
- Plant Ecology and Taxonomy Studies
- Mediterranean and Iberian flora and fauna
- Ecology and Vegetation Dynamics Studies
- Species Distribution and Climate Change
- Microbial Community Ecology and Physiology
- Phytochemistry and Biological Activities
- Protist diversity and phylogeny
- Natural product bioactivities and synthesis
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Vehicle emissions and performance
Slovak Academy of Sciences
2014-2024
Institute of Botany of the Slovak Academy of Sciences
2012-2024
Charles University
2018-2024
Institute of Chemistry of the Slovak Academy of Sciences
2014-2016
Czech Academy of Sciences, Institute of Botany
2011-2015
Abstract The European Alps are highly rich in species, but their future may be threatened by ongoing changes human land use and climate. Here, we reconstructed vegetation, temperature, impact livestock over the past ~12,000 years from Lake Sulsseewli, based on sedimentary ancient plant mammal DNA, pollen, spores, chironomids, microcharcoal. We assembled a highly-complete local DNA reference library (PhyloAlps, 3923 taxa), used this to obtain an exceptionally sed aDNA record of 366 taxa....
The phylogeographical history of mid-altitude woodland herbs that depend on moist and shaded forest habitats is poorly understood. Here, we analysed the genetic structure Cyclamen purpurascens, a mountainous calcicolous perennial, to test hypotheses regarding its glacial survival in single or multiple refugia postglacial colonization routes, explore how they are congruent with histories inferred for temperate trees other herbs. We gathered AFLP data chloroplast DNA sequences (trnD-trnT...
Mountains of the Balkan Peninsula are significant biodiversity hotspots with great species richness and a large proportion narrow endemics. Processes that have driven evolution rich mountain flora, however, still insufficiently explored understood. Here we focus on group Cardamine (Brassicaceae) perennials growing in wet, mainly mountainous habitats. It comprises several Mediterranean endemics, including those restricted to Peninsula. We used target enrichment genome skimming (Hyb-Seq) infer...
Abstract Aim Although divergences in nuclear DNA content among different species within a genus are widely acknowledged, intraspecific variation is still somewhat controversial issue. The aim of this study was to assess genome size the polymorphic Picris hieracioides L. (Asteraceae) and search for potential interpretations heterogeneity. Location Europe. Methods sizes 179 plants P. collected from 54 populations distributed across 10 European countries were determined by propidium iodide flow...
Premise Hybrid capture with high‐throughput sequencing (Hyb‐Seq) is a powerful tool for evolutionary studies. The applicability of an Asteraceae family‐specific Hyb‐Seq probe set and the outcomes different phylogenetic analyses are investigated here. Methods data from 112 samples were organized into groups at taxonomic levels (tribe, genus, species). For each group, sets non‐paralogous loci built proportions parsimony informative characters estimated. impacts analyzing alternative sets,...
Abstract The ciliate genus Spirostomum comprises eight morphospecies, inhabiting diverse aquatic environments worldwide, where they can be used as water quality indicators. Although species are relatively easily identified using morphological methods, the previous nuclear rDNA-based phylogenies indicated several conflicts in morphospecies delineation. Moreover, single locus and analytical approaches could not unambiguously resolve phylogenetic relationships among morphospecies. Here, we...
Abstract The Balkan Peninsula, with many endemic species, is known as one of the most important speciation and diversification centres in Europe. Here, we present a study western populations polymorphic European V. suavis s.l., which have been reported under name adriatica, but their taxonomic status position within genus remained uncertain. Viola s.l. nine close relatives sampled across Europe were subjected to molecular (sequencing nuclear ribosomal internal transcribed spacers amplified...
Abstract The present paper provides a large-scale taxonomic revision of Picris hieracioides, highly polymorphic and taxonomically controversial species in Europe. Altogether, 104 populations were sampled examined using multivariate morphometrics genetic amplified fragment length polymorphism (AFLP) data. Two morphotypes are delimited, the ‘higher altitude’ ‘lower morphotypes, which congruent with two main groupings revealed by AFLP markers. It is concluded that subspecies should be...
The current generally accepted concept of Russula maculata defines the species by yellow-brownish spots on basidiomata, an acrid taste, a yellow spore print and red pileus. This was tested using collections originating from various geographical areas mainly in Europe. Analyses ITS region suggested that there were three within this broad concept. One them, R. maculata, identified based sequence epitype. Two other species, nympharum sp., are described here as newly species. European grow...
Low-coverage whole genome shotgun sequencing (or skimming) has emerged as a cost-effective method for acquiring genomic data in nonmodel organisms. This provides sequence information on chloroplast (cpDNA), mitochondrial (mtDNA) and nuclear ribosomal regions (rDNA), which are over-represented within cells. However, numerous bioinformatic challenges remain to accurately rapidly obtain such organisms with complex structures rearrangements, particular mtDNA plants or cpDNA some plant families....
SUMMARY Specific ecological conditions in the high mountain environment exert a selective pressure that often leads to convergent trait evolution. Reticulations induced by incomplete lineage sorting and introgression can lead discordant patterns among gene species trees (hemiplasy/xenoplasy), providing false illusion traits under study are homoplastic. Using phylogenetic networks, we explored effect of exchange on evolution Soldanella , genus profoundly influenced historical introgression....
The paradox between the ubiquity of polyploid lineages in plants and early obstacles to establishment polyploids is a long‐studied yet unresolved question evolutionary biology. It assumed that successfully persist after emergence, newly formed need display certain fitness advantages show extent reproductive isolation with their diploid progenitors. In this study, we tested whether immediate floral changes following polyploidization can improve pollinator visitation enable preference leading...
Hybridization is a key mechanism involved in lineage diversification and speciation, especially ecosystems that experienced repeated environmental oscillations. Recently radiated plant groups, which have evolved mountain impacted by historical climate change provide an excellent model system for studying the impact of gene flow on speciation. We combined organellar (whole-plastome) nuclear genomic data (RAD-seq) with cytogenetic approach (rDNA FISH) to investigate effects hybridization...
Abstract Background and Aims Southwestern Asia is a significant centre of biodiversity cradle diversification for many plant groups, especially xerophytic elements. In contrast, little known about the evolution its hygrophytic flora. To fill this gap, we focus on Cardamine (Brassicaceae) species that grow in wetlands over wide altitudinal range. We aimed to elucidate their evolution, assess extent presumed historical gene flow between species, draw inferences intraspecific structure. Methods...
Species of Russula subsect. Xerampelinae are notoriously difficult to identify and name have not been subject molecular study. A group species, referred here as the R. clavipes complex, growing in association with Salix, Betula Populus well coniferous tree species from temperate arctic alpine habitats, were examined. Analyses nuc rDNA internal transcribed spacer (ITS) region a numerical analysis morphological characters used. The complex is monophyletic within Xerampelinae, according...
The Balkan Peninsula is one of the most important centres plant diversity in Europe. Here we aim to fill gap current knowledge evolutionary processes and factors modelling this astonishing biological richness by applying multiple approaches Cyanus napulifer group.To reconstruct mode diversification within C. group uncover its relationships with potential relatives x = 10 from Europe Northern Africa, examined variation genetic markers (amplified fragment length polymorphisms [AFLPs]; 460...
The role of endemic species in global biodiversity is pivotal, and understanding their biology ecology imperative for fitness long-term survival, particularly the face ongoing climatic oscillations. Our primary goal was to investigate sexual reproduction level endangered Western Carpathian Daphne arbuscula (Thymelaeaceae), which inhabits extreme rocky habitats, comprehend influence specific factors on its reproductive success. We conducted research across four populations, varying size...
Abstract Rocky habitats, globally distributed ecosystems, harbour diverse biota, including numerous endemic and endangered species. Vascular plants thriving in these environments face challenging abiotic conditions, requiring morphological physiological adaptations. Their engagement with the surrounding microbiomes is, however, equally vital for their adaptation, fitness, long‐term survival. Nevertheless, there remains a lack of understanding this complex interplay within fascinating biotic...
The lichen genus Solenopsora occurs predominantly in temperate and subtropical regions of the world, centre diversity distribution is Mediterranean, Macaronesian, Madrean floristic regions. Taxonomic treatment several taxa has varied over time concepts lack clarity. Focusing on multilocus sequence data, morphology, anatomy, chemistry, ecological preferences, present study investigates European to obtain first insights into their genetic variation relationships. Our results show discrepancy...
We investigated genetic variation and evolutionary history of closely related taxa Picris subsect. Hieracioides with major focus on the widely distributed P. hieracioides its congeners, hispidissima, japonica, olympica, nuristanica. Accessions from 140 sample sites were analyzed infra- inter-specific level using nuclear (ITS1-5.8S-ITS2 region) chloroplast (rpl32-trnL(UAG) DNA sequences. Genetic patterns hieracioides, olympica shown to be incongruent and, in several cases, both plastid...
We investigated ochratoxin A (OTA) contamination in raisin samples purchased from Slovak markets and determined the diversity of black-spored aspergilli as potential OTA fumonisin (FB1 FB2) producers. The taxonomic identification was performed using sequences nuclear ITS1-5.8s-ITS2 region, calmodulin beta-tubulin genes. obtained 239 isolates eight fungal genera, which 197 belonged to Aspergillus (82%) 42 strains (18%) other genera. evidenced 75% its level ranged 0.8 10.6 µg/kg. combination...