A5075554982- Environmental DNA in Biodiversity Studies
- Microbial Community Ecology and Physiology
- Isotope Analysis in Ecology
- Fish Ecology and Management Studies
- Genetic diversity and population structure
- Forensic and Genetic Research
- Identification and Quantification in Food
- Hydrocarbon exploration and reservoir analysis
- Protist diversity and phylogeny
- Geology and Paleoclimatology Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine and fisheries research
- Geochemistry and Geologic Mapping
- Aquatic Invertebrate Ecology and Behavior
Umeå University
2017-2021
The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field paleoecological research. Although largely applied nowadays, knowledge gaps remain this there therefore still research be conducted ensure reliability signal. Building on most recent literature seven original case studies, we synthesize state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation...
Abstract Measures of environmental DNA (eDNA) concentrations in water samples have the potential to be both a cost‐efficient and nondestructive method estimate fish population abundance. However, inherent temporal spatial variability abiotic biotic conditions aquatic systems been suggested major obstacle determine relationships between eDNA Moreover, once are collected, methodological biases common, which introduces additional sources variation Here, we evaluate performance applying droplet...
DNA preserved in sedimentary materials can be used to study past ecosystem changes, such as species' colonization and extinction. It is believed that minerals, especially clay enhance the preservation of DNA. However, role well organic matter, on sorption heterogeneous sediments still not clear. In this study, we examined effect mineral matter binding lake sediments. Bulk size-fractionated (0–4, 4–16, 16–64, >64 μm), having different composition, were test sorption; similar experiments also...
Abstract Detection of DNA in lake sediments holds promise as a tool to study processes like extinction, colonization, adaptation and evolutionary divergence. However, low concentrations make sediment difficult detect, leading high false negative rates. Additionally, contamination could potentially lead positive Careful laboratory procedures can reduce rates, but should not be assumed completely eliminate them. Therefore, methods are needed that identify potential results, use this...
Abstract Purpose Intact lake sediments reflect the development of terrestrial ecosystems. This can be understood by decoding mineral and geochemical information sedimentary archives. Therefore, we characterized a Holocene sediment core revealed bulk to micro-scale variations via combination techniques statistical methods. Methods A 2.3 m was collected from Hotagen, in west-central Sweden; sample every 5 cm. part each kept untreated (named bulk) another size-fractionated into < 4, 4–16,...
Abstract Loss of habitat and changes in the spatial configuration habitats are major drivers species extinctions, but responses to these differ between organisms. To advance theory on how extinction risk from different types alteration relates species‐specific traits, there is a need for studies long‐term dynamic individual species. The goal this study was quantify area affect rate an aquatic top predator, northern pike Esox lucius L. We recorded presence/absence 398 isolated fragments, each...
Abstract Sedimentary environmental DNA (sed-eDNA) coupled with metabarcoding is increasingly exploited for ecological studies, but application of the method to resolve fish dynamics in lakes still needs better validation. This study (1) evaluated sed-eDNA yields from commonly used DNeasy PowerSoil Kit mineral-rich and organic-rich sediments (2) examined viability recovery detection surface sediment samples 13 Swedish mountain lakes, organic contents 18–52%, by using conventional PCR droplet...