A5003564286- Genetic diversity and population structure
- Amphibian and Reptile Biology
- Species Distribution and Climate Change
- Molecular Biology Techniques and Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Evolution and Genetic Dynamics
- Fish Ecology and Management Studies
- Animal and Plant Science Education
- Ecology and Vegetation Dynamics Studies
- Wildlife Ecology and Conservation
Naturalis Biodiversity Center
2014-2024
Leiden University
2021-2024
Vrije Universiteit Brussel
2014-2015
University of Salford
2015
Habitat loss, together with less obvious land-use changes such as intensified farming practice, can have significant adverse impacts on biodiversity. An important factor in determining the ability of species to cope is their potential sustain a populations network by dispersal across landscape. quality and structure are particularly for surface-dwelling low abilities, amphibians. To assess this ecological function, ponds coastal typically rural area northern France were surveyed amphibians...
Abstract Next‐generation sequencing is a fast and cost‐effective way to obtain sequence data for nonmodel organisms many markers individuals. We describe protocol through which we orthologous the crested newts (Amphibia: Salamandridae: Triturus ), suitable analysis of interspecific hybridization. use transcriptome single species design 96 primer pairs that amplify c . 180 bp fragments positioned in 3‐prime untranslated regions. Next, these are tested with uniplex PCR set spanning taxonomical...
Genetic pollution of a native species through hybridization with an invasive poses insidious conservation threat. To expose genetic pollution, molecular methods employing multilocus data are required. We present case study via crested newt species, Triturus cristatus, by the carnifex on Veluwe in Netherlands. sequenced 50 nuclear markers next generation sequencing and one mitochondrial marker Sanger for four populations from range both parent eleven ponds Veluwe. use three population...
Abstract Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance such populations. The alpine newt has across Netherlands, United Kingdom and Ireland. However, geographical mtDNA structure natural range still incompletely understood certain regions are severely undersampled. We collect sequence data over seven hundred individuals, from both main new insights our extended phylogeography that 1) haplotypes...
Abstract We introduce multiplex Kompetitive Allele-Specific PCR (mxKASP): a modification of ‘classical’ KASP genotyping that allows zygosity to be determined in diploid organisms. Rather than targeting SNP associated with single marker, mxKASP targets two non-homologous markers. show proof concept by applying the balanced lethal system Triturus newts, which individuals are known possess either: (1) zero copies 1A version chromosome 1 and 1B version; (2) one copy 1B; or (3) 1B. is successful...
When a female crested newt lays her clutch of about three-hundred eggs, half these will never hatch. What an incredible waste! It turns out that newts suffer from deadly hereditary disease called balanced lethal system. Here is how it works: in system, there are two distinct versions particular chromosome. Newts need both to live. However, which version fertilized egg gets its father and mother random. Therefore, 50% chance receives the same twice—and misses other critical pair. Hence, eggs...