A5058759130- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Neonatal Respiratory Health Research
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Eosinophilic Esophagitis
- Nuclear physics research studies
- Drug-Induced Hepatotoxicity and Protection
- Nuclear Physics and Applications
- Probiotics and Fermented Foods
- Pediatric health and respiratory diseases
- Effects and risks of endocrine disrupting chemicals
- Occupational exposure and asthma
- Immunotherapy and Immune Responses
- Barrier Structure and Function Studies
- Radioactive element chemistry and processing
- Protein Hydrolysis and Bioactive Peptides
- Immune Response and Inflammation
- Drug Transport and Resistance Mechanisms
- Extraction and Separation Processes
- Biochemical Analysis and Sensing Techniques
Utrecht University
2012-2022
Netherlands Environmental Assessment Agency
2019
HAL Allergy (Netherlands)
2016
University of Michigan–Ann Arbor
2005-2013
Michigan United
2008
University of California, Riverside
2005
University of Redlands
2005
National Institute for Public Health and the Environment
2003
Food & Nutrition
1999
North-West University
1973-1998
Respiratory syncytial virus (RSV) infection is widely spread and a major cause of bronchiolitis in infants high-risk adults, often leading to hospitalization. RSV leads obstruction inflammation the airways induction innate acquired immune responses. Because dendritic cells (DCs) are essential elicitation these responses, we investigated presence role cell subtypes upon lung. Here, report that increased number both conventional plasmacytoid lung lung-draining lymph nodes. In particular,...
The detection of a viral infection by pattern recognition receptors (PAMPs) is an integral part antiviral immunity. In these studies we have investigated the role TLR3, which recognizes dsRNA, in Respiratory Syncytial virus (RSV) using B6 background mice with TLR3 deletion. Although observed no changes growth, did find that TLR3-/- demonstrated significant increases mucus production airways RSV-infected mice. qualitative assessment was examining differentially stained lungs, followed...
It was previously shown that administration of recombinant human Fms-like tyrosine kinase receptor-3 ligand (Flt3L) before allergen challenge sensitized mice suppresses the cardinal features asthma through unclear mechanisms. Here, we show Flt3L dramatically alters balance conventional to plasmacytoid dendritic cells (pDCs) in lung favoring accumulation pDCs. Selective removal pDCs abolished antiinflammatory effect Flt3L, suggesting a regulatory role for these ongoing asthmatic inflammation....
Abstract Background The major risk factor for the development of COPD is cigarette smoking. Smoking causes activation resident cells and recruitment inflammatory into lungs, which leads to release pro-inflammatory cytokines, chemotactic factors, oxygen radicals proteases. In present study evidence found a new cellular mechanism that refers link between smoking inflammation in lungs. Methods Employing human monocyte-derived macrophages, different techniques including FACS analysis, Cytometric...
The response to respiratory syncytial virus (RSV), negative strand ssRNA virus, depends upon the ability recognize specific pathogen-associated targets. In current study, role of TLR7 that recognizes was examined. Using TLR7(-/-) mice, we found RSV infection in lung more pathogenic as assessed by significant increases inflammation and mucus production. Although there appeared be no effect deficiency on type I IFN, pathology associated with an alteration T cell responses mucogenic cytokines...
The current allergenicity assessment of novel proteins is based on the EFSA GMO guidance. Recently, launched a new guidance document GM plants (2017). This describes, amongst other topics, scientific and regulatory developments in vitro protein digestibility tests. Panel stated that for tests, additional investigations are needed before any recommendation form can be provided. To this end, an interim phase considered necessary to evaluate revisions gastrointestinal digestion test, proposed...
BackgroundRespiratory syncytial virus (RSV) infects nearly all infants by age 2 and is a leading cause of bronchiolitis. RSV may employ several mechanisms to induce immune dysregulation, including dendritic cell (DC) modulation during the response RSV.Methods FindingsExpansion cDC pDC Flt3L treatment promoted an anti-viral with reduced pathophysiology characterized decreased airway hyperreactivity, Th2 cytokines, increased Th1 reduction in inflammation mucus overexpression. These protective...
Chronic obstructive pulmonary disease (COPD) is characterized by chronic airway inflammation. Cigarette smoke has been considered a major player in the pathogenesis of COPD. The inflamed airways COPD patients contain several inflammatory cells including neutrophils, macrophages,T lymphocytes, and dendritic (DCs). relative contributions these various to injury remodeling are not well documented. In particular, potential role DCs as mediators inflammation smoker's poorly understood. current...
Cross-linking of proteins has been exploited by the food industry to change texture and functionality but effects these manipulations on allergenicity still remain unclear. To model safety assessment biopolymers, we created cross-linked bovine β-lactoglobulin (CL-BLG) laccase treatment. The purpose present study was compare immunogenicity CL-BLG with native BLG in a mouse allergy. First, BALB/c mice were intragastrically sensitized orally challenged or BLG-specific serum antibodies splenic...
Scope During food processing, the Maillard reaction (МR) may occur, resulting in formation of glycated proteins. Glycated proteins are particular importance allergies because glycation influence interactions with immune system. This study compared native and extensively milk allergen β‐lactoglobulin (BLG), their cells crucially involved allergy. Methods results BLG was MR characterized. Native were tested experiments epithelial transport, uptake degradation by DCs, T‐cell cytokine responses,...
Abstract Respiratory syncytial virus (RSV) is the leading cause of respiratory disease in infants worldwide. The induction innate immunity and establishment adaptive immune responses are influenced by recognition pathogen-associated molecular patterns TLRs. One primary pathways for TLR activation MyD88 adapter protein signaling. present studies indicate that deficiency profoundly impacts pulmonary environment RSV-infected mice characterized accumulation eosinophils augmented mucus...
Cite this as : J. Smit, M. Bol‐Schoenmakers, I. Hassing, D. Fiechter, L. Boon, R. Bleumink and H. Pieters, Clinical & Experimental Allergy , 2011 (41) 890–898. Summary Background Food allergy affects approximately 6% of children is the leading cause hospitalization for anaphylactic reactions in westernized countries. Crucial establishment activation dendritic cells (DC) to T helper 2‐mediated responses. Objective We, therefore, investigated whether changes DC subsets precede food...
Food allergy is an increasing health problem in Western countries. Previously, it has been shown that the intensity of food allergic reactions can be regulated by regulatory T (Treg) cells. In addition, activation aryl hydrocarbon receptor (AhR) regulates T-cell responses induction Treg Therefore, we hypothesized AhR pathway suppress development through This was investigated using a mouse model for peanut allergy. C3H/HeOuJ mice (AhRb-2) were sensitized to administering extract (PE) gavage...
Food allergy affects approximately 5% of children and is the leading cause hospitalization for anaphylactic reactions in westernized countries. However, pathways anaphylaxis food are still relatively unknown. We investigated effector allergic responses different strains mice a clinical relevant model peanut allergy. C3H/HeOuJ, C57BL/6 BALB/c were sensitized by intragastric extract challenged or intraperitoneal injection peanut. Peanut-specific T cell responses, IgE, IgG1 IgG2a mucosal mast...
Abstract Background Peanuts are most responsible for food‐induced anaphylaxis in adults developed countries. An effective and safe immunotherapy is urgently needed. The aim of this study was to investigate the immunogenicity, allergenicity, immunotherapeutic efficacy a well‐characterized chemically modified peanut extract ( MPE ) adsorbed Al( OH 3 . Methods Peanut PE by reduction alkylation. Using sera peanut‐allergic patients, competitive IgE‐binding assays mediator release were performed....
Background: Oral immunotherapy (OIT) is a promising therapeutic approach to treat food allergic patients. However, there are some concerns regarding its safety and long-term efficacy. The use of non-digestible oligosaccharides might improve OIT efficacy since they known directly modulate intestinal epithelial- immune cells in addition acting as prebiotics. Aim: To investigate whether diet supplemented with plant-derived fructo-oligosaccharides (FOS) supports the murine cow's milk allergy...
In previous studies, we showed that a fructo-oligosaccharide- (FOS-) supplemented diet enhanced oral immunotherapy (OIT) efficacy in mouse model for cow's milk allergy. Fermentation of FOS by intestinal bacteria leads to production short-chain fatty acids (SCFA) including butyrate.To investigate the contribution butyrate OIT + FOS.C3H/HeOuJ mice were sensitized and received with or without supplementation. After treatment, whole blood was collected conduct basophil activation test (BAT)...
To cite this article: Bol-Schoenmakers M, Marcondes Rezende Bleumink R, Boon L, Man S, Hassing I, Fiechter D, Pieters RHH, Smit JJ. Regulation by intestinal γδ T cells during establishment of food allergic sensitization in mice. Allergy 2011; 66: 331–340. Background: Food allergy affects approximately 5% children and is the leading cause hospitalization for anaphylactic reactions westernized countries. The mucosal adjuvant cholera toxin induces to co-administered proteins mice, while feeding...