A5069482286- Food Allergy and Anaphylaxis Research
- Probiotics and Fermented Foods
- Eosinophilic Esophagitis
- Pediatric health and respiratory diseases
- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- IL-33, ST2, and ILC Pathways
- Protein Hydrolysis and Bioactive Peptides
- Gut microbiota and health
- Respiratory Support and Mechanisms
- COVID-19 and Mental Health
- Neonatal Respiratory Health Research
- Allergic Rhinitis and Sensitization
- Eosinophilic Disorders and Syndromes
- Gastrointestinal motility and disorders
- Wound Healing and Treatments
- Cytokine Signaling Pathways and Interactions
- Education and Learning Interventions
- Exercise and Physiological Responses
- Genomics, phytochemicals, and oxidative stress
- Mast cells and histamine
- Glycosylation and Glycoproteins Research
- Diabetic Foot Ulcer Assessment and Management
- Escherichia coli research studies
- Animal health and immunology
Utrecht University
2014-2024
Pharmo Institute
2020
Introduction Pulmonary neutrophilia is a hallmark of numerous airway diseases including Chronic Obstructive Disease (COPD), Neutrophilic asthma, Acute Lung Injury (ALI), Respiratory Distress Syndrome (ARDS) and COVID-19. The aim the current study was to investigate effect dietary interventions on lung health in context pulmonary neutrophilia. Methods Male BALB/cByJ mice received 7 intra-nasal doses either vehicle or lipopolysaccharides (LPS). To nutritional they 16 intra-gastric (PBS)...
Several studies demonstrated the adverse effect of milk processing on allergy-protective capacity raw cow's milk. Whether also affects allergenicity is hardly investigated.To assess (unprocessed) and processed in a murine model for food allergy as well allergic children.C3H/HeOuJ mice were either sensitized to whole (raw milk, heated or shop [store-bought milk]) challenged with protein they whey proteins (native heated). Acute symptoms, mast cell degranulation, allergen-specific IgE levels...
Epidemiological studies show an inverse relation between raw cow's milk consumption and the development of asthma. This protective effect seems to be abolished by processing. However, evidence for a causal relationship is lacking, direct comparisons processed are hardly studied. Therefore, this study investigated preventive capacity heated on house dust mite (HDM)-induced allergic asthma in mice. Six- seven-week-old male BALB/c mice were intranasally (i.n.) sensitized with 1 µg HDM or PBS...
Background: Oral immunotherapy (OIT) is a promising therapeutic approach to treat food allergic patients. However, there are some concerns regarding its safety and long-term efficacy. The use of non-digestible oligosaccharides might improve OIT efficacy since they known directly modulate intestinal epithelial- immune cells in addition acting as prebiotics. Aim: To investigate whether diet supplemented with plant-derived fructo-oligosaccharides (FOS) supports the murine cow's milk allergy...
Epidemiological studies identified raw cow's milk consumption as an important environmental exposure that prevents allergic diseases. In the present study, we investigated whether has capacity to induce tolerance unrelated, non-milk, food allergen. Histone acetylation of T cell genes was assess potential epigenetic regulation. Female C3H/HeOuJ mice were sensitized and challenged ovalbumin. Prior sensitization, treated with milk, processed or phosphate-buffered saline for eight days. Allergic...
Mast cells (MC) accumulate in colorectal cancer (CRC) and the relationship between MC density progression has been well recognized. can be either pro-tumor or anti-tumor players, depending on local factors present tumor microenvironment. Upon malignant transformation, express high levels of sialic acids cell membrane by secretion. Siglecs are a family immunoglobulin-like receptors that bind each subtype distinct pattern expression immune cells. Among them, Siglec-6 is expressed predominately...
In previous studies, we showed that a fructo-oligosaccharide- (FOS-) supplemented diet enhanced oral immunotherapy (OIT) efficacy in mouse model for cow's milk allergy. Fermentation of FOS by intestinal bacteria leads to production short-chain fatty acids (SCFA) including butyrate.To investigate the contribution butyrate OIT + FOS.C3H/HeOuJ mice were sensitized and received with or without supplementation. After treatment, whole blood was collected conduct basophil activation test (BAT)...
Non-communicable diseases are increasing and have an underlying low-grade inflammation in common, which may affect gut health. To maintain intestinal homeostasis, unwanted epithelial activation needs to be avoided. This study compared the efficacy of butyrate, propionate acetate suppress IFN-γ+/-TNF-α induced association with their HDAC inhibitory capacity, while studying canonical non-canonical STAT1 pathway. HT-29 were activated treated short chain fatty acids (SCFAs) or histone...
Mast cells (MCs) accumulate in the epithelium of patients with eosinophilic esophagitis (EoE), an inflammatory disorder characterized by extensive esophageal infiltration. Esophageal barrier dysfunction plays important role pathophysiology EoE. We hypothesized that MCs contribute to observed impaired epithelial barrier. Herein, we demonstrate coculture differentiated immunoglobulin E-activated significanly decreased resistance 30% and increased permeability 22% compared non-activated MCs....
Abstract To support dietary management of severe cow's milk allergic infants, a synbiotic mixture non‐digestible oligosaccharides and Bifidobacterium breve M‐16V ( B. ) was designed from source materials that are completely milk‐free. It investigated whether this specific concept can reduce an established food response in research model for hen's egg allergy. Mice were orally sensitized once week 5 weeks to ovalbumin (OVA) using cholera toxin (CT) as adjuvant. Non‐sensitized mice received CT...
Raw cow's milk was previously shown to suppress allergic symptoms in a murine model for food allergy. In the present study, we investigated contribution of fat content and heat-sensitive components this allergy-protective effect. addition, determined potency alkaline phosphatase (ALP), raw component, affect response. C3H/HeOuJ mice were treated with milk, pasteurized skimmed spiked ALP, or phosphate-buffered saline eight days prior sensitization challenge ovalbumin (OVA). Effects these types...
Whey proteins denaturing ≤65 °C are important for allergy protection by raw cow's milk.
This study aims to characterize the microbiota and peptidomic composition of raw milk kefir, address potential anti-allergic effects kefir using validated research models for food allergy. Raw was produced after incubation with a defined freeze-dried starter culture. Kefir sampled during fermentation at seven time intervals. For comparison, also prepared from heat-treated milk. Peptide compositions were determined heated milk, end products made these milks. In murine allergy model, two...
Cow's milk allergy (CMA) prevails in infants and brings increased risk of developing other allergic diseases. Oral administration specific β-lactoglobulin (BLG)-derived peptides (PepMix) a blend short- long-chain fructo-oligosaccharides Bifidobacterium breve M-16V (FF/Bb) was found to partially prevent CMA development mice. In this study, we aimed expand the knowledge on preventive potential underlying mechanisms approach. Three-week-old female C3H/HeOuJ mice were orally exposed PepMix±FF/Bb...
Background Early life provides a window of opportunity to prevent allergic diseases. With prevalence 0.5–2% in infants, hen’s egg allergy is one the most common food allergies. The immunomodulatory effects human milk oligosaccharides (HMOs), 2′-fucosyllactose (2’FL), and 3-fucosyllactose (3FL) were studied an vitro mucosal immune model vivo murine for (ovalbumin) allergy. Methods Intestinal epithelial cell (IEC)/dendritic (DC) DC/T cocultures used expose IECs ovalbumin (OVA) model....
Abstract Background Eosinophilic esophagitis (EoE) is an allergen‐mediated disease and elimination diets have proven to be effective obtain clinical histological remission. However, the effect of on specific EoE transcripts their correlates relatively unknown. The main aim study was evaluate dietary treatment (four‐food diet [FFED]) with or without addition amino acid‐based formula (AAF) a variety pro‐/anti‐inflammatory, epithelial/barrier function remodeling/fibrosis‐related markers...
One cluster of the extrapulmonary manifestations in chronic obstructive pulmonary disease (COPD) is related to brain, which includes anxiety, depression and cognitive impairment. Brain-related comorbidities are worsening symptoms increased mortality COPD patients. In this study, a murine model was used examine effects emphysema repetitive inflammatory events on systemic outcomes brain function. addition, effect dietary intervention brain-related parameters assessed. Adult male C57Bl/6J mice...
It is vital that Immune fitness, i.e., how well the immune system functions and reacts to challenges, can be reliably examined. The current study aimed compare fitness with assessments of saliva biomarkers systemic inflammation.N = 108 healthy young adults (18-30-year-old students Utrecht University, Netherlands) participated in study. A sample was collected for biomarker assessment (Interleukin (IL)-1β, IL-6, IL-8, IL-10, immunoglobulin (IgA), tumor necrosis factor-alpha (TNF-α), c-reactive...
The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides omega-3 poly-unsaturated fatty acids (n-3 PUFAs). These affect immune function, brain development behaviour. We investigated additive effect a dietary combination scGOS:lcFOS n-3 PUFAs on caecal content system, behaviour from day birth to early adulthood in healthy mice. Male BALB/cByJ mice received control or enriched...