A5004676463- Mast cells and histamine
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Monoclonal and Polyclonal Antibodies Research
- Dermatology and Skin Diseases
- Contact Dermatitis and Allergies
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Eosinophilic Disorders and Syndromes
- IL-33, ST2, and ILC Pathways
- Proteins in Food Systems
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- Viral gastroenteritis research and epidemiology
- Cell death mechanisms and regulation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Eosinophilic Esophagitis
- Immunotherapy and Immune Responses
- Circular RNAs in diseases
- Infant Nutrition and Health
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Utrecht University
2013-2024
Cancer Genomics Centre
2019
Short-chain fatty acids (SCFAs) are fermented dietary components that regulate immune responses, promote colonic health, and suppress mast cell-mediated diseases. However, the effects of SCFAs on human cell function, including underlying mechanisms, remain unclear. Here, we investigated (acetate, propionate, butyrate) pathology activation, molecular mechanisms involved.Precision-cut lung slices (PCLS) allergen-exposed guinea pigs were used to assess butyrate allergic airway contraction....
Rationale: Neutrophils are key players in chronic obstructive pulmonary disease (COPD), and increased numbers of neutrophils present sputum lung tissue patients with COPD. Interestingly, immunoglobulin free light chains (IgLC) able to prolong the life neutrophils; therefore, IgLC may contribute state inflammation.Objectives: In this study, relation between COPD has been investigated.Methods: We investigated presence different murine emphysema models. levels serum from mice were examined by...
Chronic inflammation drives the development of colorectal cancer (CRC), where tumor-infiltrating immune cells interact with in a dynamic crosstalk. Mast (MC), one earliest recruited cells, accumulate CRC tissues and their density is correlated progression. However, exact contribution MC interaction colon poorly understood. Here, we investigated impact primary human mediators on growth using 2D 3D coculture models. Primary were generated from peripheral CD34+ stem cells. Transwell chambers...
Mast cells (MC) accumulate in colorectal cancer (CRC) and the relationship between MC density progression has been well recognized. can be either pro-tumor or anti-tumor players, depending on local factors present tumor microenvironment. Upon malignant transformation, express high levels of sialic acids cell membrane by secretion. Siglecs are a family immunoglobulin-like receptors that bind each subtype distinct pattern expression immune cells. Among them, Siglec-6 is expressed predominately...
In previous studies, we showed that a fructo-oligosaccharide- (FOS-) supplemented diet enhanced oral immunotherapy (OIT) efficacy in mouse model for cow's milk allergy. Fermentation of FOS by intestinal bacteria leads to production short-chain fatty acids (SCFA) including butyrate.To investigate the contribution butyrate OIT + FOS.C3H/HeOuJ mice were sensitized and received with or without supplementation. After treatment, whole blood was collected conduct basophil activation test (BAT)...
Mast cells (MCs) accumulate in the epithelium of patients with eosinophilic esophagitis (EoE), an inflammatory disorder characterized by extensive esophageal infiltration. Esophageal barrier dysfunction plays important role pathophysiology EoE. We hypothesized that MCs contribute to observed impaired epithelial barrier. Herein, we demonstrate coculture differentiated immunoglobulin E-activated significanly decreased resistance 30% and increased permeability 22% compared non-activated MCs....
Traditionally, mast cells were regarded as key orchestrating type I hypersensitivity responses. However, it is now recognized that are widely involved in nonallergic (non-IgE) chronic diseases. Also, inflammatory bowel disease (IBD), a not associated with increased IgE concentrations, clear signs of activation have been found. In this study, we investigated if Ig-free L chain-induced hypersensitivity-like responses through could contribute to the pathophysiology IBD. As cell-dependent model...
Idiopathic pulmonary fibrosis (IPF), a devastating lung disorder of unknown aetiology, and chronic hypersensitivity pneumonitis (HP), disease provoked by an immunopathologic reaction to inhaled antigens, are two common interstitial diseases with uncertain pathogenic mechanisms. Previously, we have shown in other upper lower airway that immunoglobulin free light chains (FLCs) increased may be involved initiating local inflammation. In this study explored if such mechanism also apply HP IPF.In...
// Tom Groot Kormelink 1,* , Desmond G. Powe 2,* Sylvia A. Kuijpers 3 Abulikemu Abudukelimu Marcel H.A.M. Fens Ebel H.E. Pieters Willemiek W. Kassing-van der Ven Hany O. Habashy 4 Ian Ellis 5 Bart R. Blokhuis 1 Marco Thio Wim E. Hennink Gert Storm Frank Redegeld 1,7 and Raymond M. Schiffelers 6,7 Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty Science, University, Utrecht, The Netherlands 2 Department Cellular Pathology, Queen's Medical Centre, Nottingham...
Previously, the protective farm effect was imitated using whey protein beta-lactoglobulin (BLG) that is spiked with iron-flavonoid complexes. Here, we formulated for clinical translation a lozenge as food special medical purposes (FSMP) catechin-iron complexes ligands BLG. The tested in vitro and therapeutical BALB/c mice model.Binding of iron-catechin into BLG confirmed by spectroscopy docking calculations. Serum IgE binding children allergic or tolerating milk assessed to loaded (holo-)...
Allergic diseases have become a serious global health problem in the developed world. IgE interacting with its high-affinitiy receptor FcepsilonRI is considered major contributing factor to most types of allergies, but depending on type allergy, however, subgroup patients displays common symptoms and yet lack elevated levels total serum and/or antigen-specific IgE. Novel therapeutic strategies such as anti-IgE therapy may therefore not be applicable these patients. It clear, that do display...
Beside the production of complete immunoglobulins IgG, IgE, IgA, IgM and IgD, consisting tetrameric heterodimers immunoglobulin heavy light chains, B cells also secrete free chains (Ig-fLC). Previous studies showed that Ig-fLCs are able to induce immediate hypersensitivity reactions. It is apparent recognition binding antigen crucial steps in onset these inflammatory responses. In this study, characteristics Ig-fLC were further investigated using various biochemical approaches. addition, we...
Symptoms of allergy are largely attributed to an IgE-mediated hypersensitivity response. However, a considerable number patients also exhibit clinical features without detectable systemic IgE. Previous work showed that Ig-free light chains (IgLC) may act as alternate mechanism induce allergic responses. CD4+CD25+ T cells crucial in the initiation and regulation responses compromised function might affect response allergens.To examine contribution IgLC towards whey-allergic response.Mice were...
Mast cells (MCs) are one of the first immune recruited to a tumor. It is well recognized that MCs accumulate in colon cancer lesion and their density associated with clinical outcomes. However, molecular mechanism how may modify MC function still unclear. In this study, primary human were generated from CD34⁺ progenitor 3D coculture model was developed study interplay between MCs. By comparing transcriptomic profile cancer-cocultured versus control MCs, we identified number deregulated...
Bet v 1 is the major allergen in birch pollen to which up 95% of patients sensitized respond. As a member pathogenesis-related PR 10 family, its natural function implicated plant defense, with PR10 family being reported be upregulated under iron deficiency. such, we assessed sequester and immunomodulatory properties on human immune cells. Binding quercetin complexes FeQ2 was determined docking calculations by spectroscopy. Serum IgE-binding (holoBet v1) without ligands (apoBet 1) were ELISA,...
Chronic obstructive pulmonary disease (COPD) is a multicomponent characterized by emphysema and/or chronic bronchitis. COPD mostly associated with cigarette smoking. Cigarette smoke contains over 4,700 chemical compounds, including free radicals and LPS (a Toll-Like Receptor 4 agonist) at concentrations which may contribute to the pathogenesis of diseases like COPD. We have previously shown that short-term exposure medium (CSM) can stimulate several inflammatory cells via TLR4 CSM reduces...
Introduction Early life exposure to non-digestible oligosaccharides (NDO) or microbial components is known affect immune development. NDO in combination with a TLR9 agonist mimicking bacterial triggers (CpG) promoted the secretion of galectins through unknown pathways. We aimed study contribution exosomes epithelial galectin and subsequent immunoregulation upon mixture by inhibiting exosome biogenesis. Methods Human intestinal cells (IEC) (FHs 74 Int HT-29) were apically exposed...