A5087111637- Platelet Disorders and Treatments
- Blood groups and transfusion
- Blood Coagulation and Thrombosis Mechanisms
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Antiplatelet Therapy and Cardiovascular Diseases
- Chronic Lymphocytic Leukemia Research
- Venous Thromboembolism Diagnosis and Management
- Hematopoietic Stem Cell Transplantation
- Blood properties and coagulation
- Acute Myeloid Leukemia Research
- Blood disorders and treatments
- Chronic Myeloid Leukemia Treatments
- Autoimmune Bullous Skin Diseases
- Blood transfusion and management
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Dialysis and Renal Disease Management
- Renal Diseases and Glomerulopathies
- Blood donation and transfusion practices
- Cancer Treatment and Pharmacology
- Protease and Inhibitor Mechanisms
- Lipoproteins and Cardiovascular Health
- Cell Adhesion Molecules Research
- Multiple Myeloma Research and Treatments
- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
Centro Regional de Hemodonación
2016-2025
Centre for Biomedical Network Research on Rare Diseases
2016-2025
Universidad de Murcia
2016-2025
Instituto de Salud Carlos III
2016-2025
Hospital General Universitario Morales Meseguer
2012-2025
Centro de Investigación Biomédica en Red
2017-2024
Instituto Murciano de Investigación Biosanitaria
2018-2024
Instituto de Investigación de Enfermedades Raras
2016-2022
Clinica Universidad de Navarra
2021
Children's Hospital of Orange County
2021
During peritoneal dialysis (PD), mesothelial cells undergo mesothelial-to-mesenchymal transition (MMT), a process associated with peritoneal-membrane dysfunction. Because TGF-β1 can induce MMT, we evaluated the efficacy of TGF-β1-blocking peptides in modulating MMT and ameliorating damage mouse model PD. Exposure peritoneum to PD fluid induced fibrosis, angiogenesis, functional impairment, accumulation fibroblasts. In addition expressing fibroblast-specific protein-1 (FSP-1), some...
Excessive bleeding at surgery is a feared complication in patients with inherited platelet disorders. However, very few studies have evaluated the frequency of surgical these hemorrhagic We performed worldwide, multicentric, retrospective study to assess complications surgery, preventive and therapeutic approaches adopted, their efficacy disorders: Surgery Platelet disorders And Therapeutic Approach (SPATA) study. rated outcome 829 procedures carried out 423 well-defined forms 238 function...
Inherited platelet disorders are a heterogeneous group of rare diseases, caused by inherited defects in production and/or function. Their genetic diagnosis would benefit clinical care, prognosis and preventative treatments. Until recently, this has usually been performed via Sanger sequencing limited number candidate genes. High-throughput is revolutionizing the including bleeding disorders. We have designed novel high-throughput platform to investigate unknown molecular pathology cohort 82...
Summary There is no diagnostic test for primary immune thrombocytopenia (ITP). Certain microRNAs have shown to potential in ITP. We validated 12 identified from two previous studies find a biomarker. The study included ITP cohorts ( n = 61) and healthy controls 28). first cohort involved 24 patients the Prolong study, with newly diagnosed/persistent (<1 year) treated corticosteroids ± IVIG but relapsed/failed respond. second comprised 37 biobank, Østfold Hospital, Norway, had different...
Background. During peritoneal dialysis (PD), mesothelial cells (MC) undergo an epithelial-to-mesenchymal transition (EMT), and this process is associated with membrane (PM) damage. Bone morphogenic protein-7 (BMP-7) antagonizes transforming growth factor (TGF)-β1, modulates EMT protects against fibrosis. Herein, we analysed the modulating role of BMP-7 on MC in vitro its protective effects a rat PD model. Methods. Epitheliod or non-epitheliod were for expression BMP-7, TGF-β1, activated...
Epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells is a pathological process that occurs during dialysis. EMT leads to fibrosis, ultrafiltration failure and eventually the discontinuation therapy. Signaling pathways involved in are thus great interest, but mostly unknown. We used primary from human omentum analyze role p38 MAPK signaling pathway induction EMT. The use specific inhibitors, dominant-negative mutant lentiviral silencing p38α demonstrated promotes...
Serine protease inhibitors (serpins) include thousands of structurally conserved proteins playing key roles in many organisms. Mutations affecting serpins may disturb their conformation, leading to inactive forms. Unfortunately, conformational consequences serpin mutations are difficult predict. In this study, we integrate experimental data patients with one the predictions obtained by AlphaFold and molecular dynamics. Five SERPINC1 causing antithrombin deficiency, strongest congenital...
Previous studies using washed platelets demonstrated that certain flavonoids inhibit platelet function through several mechanisms including blockade of TxA2 receptors (TPs). We aimed to analyze the binding capacity TPs in rich plasma (PRP), investigated their effect flowing blood, and evaluated ability apigenin improve efficacy aspirin inhibition aggregation. The PRP was explored assays TP antagonist [3H]SQ29548. Effects on adhesion were assessed arterial subendothelium with annular plate...
Background. Peritoneal membrane deterioration during peritoneal dialysis (PD) is associated with epithelial-to-mesenchymal transition (EMT) of mesothelial cells (MC), which believed to be mainly due glucose degradation products (GDPs) present in PD solutions. Here we investigate the impact GDPs solutions on EMT MC vitro and ex vivo.
What is already known about this subject • Flavonoids are largely recognized as potential inhibitors of platelet function, through nonspecific mechanisms such antioxidant activity and/or inhibition several enzymes and signalling proteins. In addition, we, few others, have shown that certain antiaggregant flavonoids may behave specific TXA2 receptor (TP) ligands in platelets. Whether interact with TP isoforms other cell types not known, direct evidence flavonoid–TP interaction inhibits...
Mutations in ceramide biosynthesis pathways have been implicated a few Mendelian disorders of keratinization, although ceramides are known to key roles several biological processes skin and other tissues. Using whole-exome sequencing four probands with undiagnosed hyperkeratosis/ichthyosis, we identified compound heterozygosity for mutations KDSR, encoding an enzyme the de novo synthesis pathway ceramides. Two individuals had hyperkeratosis confined palms, soles, anogenital skin, whereas two...
Patients with multirefractory immune thrombocytopenia (ITP) have limited treatment options. Recent data suggest that specific anti-platelet antibodies may cause destruction of platelets by favoring platelet loss sialic acid. In this multicenter study 35 patients ITP, including 16 disease, were analyzed for antiplatelet-antibodies, thrombopoietin (TPO) levels, and desialylation. selected cases, responses to a novel strategy using oseltamivir tested.We found against GPIbα overrepresented in...
Molecular testing of Inherited bleeding coagulation disorders (IBCDs) not only offers confirmation diagnosis but also aids in genetic counselling, prenatal and certain cases genotype-phenotype correlations are important for predicting the clinical course disease to allow tailor-made follow-up individuals. Until recently, genotyping has been mainly performed by Sanger sequencing, a technique known be time consuming expensive. Currently, next-generation sequencing (NGS) new potential approach...
Abstract SARS-CoV-2 infection increases the risk of thrombosis by different mechanisms not fully characterized. Although still debated, an increase in D-dimer has been proposed as a first-line hemostasis test associated with thromboembolic and unfavorable prognosis. We aim to systematically comprehensively evaluate association between thrombin generation parameters inflammatory hypercoagulable state, well their prognostic value COVID-19 patients. A total 127 hospitalized patients confirmed...