A5090812610- Cancer-related molecular mechanisms research
- Platelet Disorders and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- Eosinophilic Disorders and Syndromes
- Acute Myeloid Leukemia Research
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Chronic Lymphocytic Leukemia Research
- RNA Research and Splicing
- Complement system in diseases
- Antiplatelet Therapy and Cardiovascular Diseases
- Erythrocyte Function and Pathophysiology
- Kruppel-like factors research
- Chronic Myeloid Leukemia Treatments
- Blood disorders and treatments
- Multiple Myeloma Research and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- Cellular transport and secretion
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Genomics and Diagnostics
- Protease and Inhibitor Mechanisms
Universidad de Murcia
2014-2025
Centro Regional de Hemodonación
2016-2025
Hospital General Universitario Morales Meseguer
2015-2025
Universidad Católica San Antonio de Murcia
2020-2025
Centre for Biomedical Network Research on Rare Diseases
2016-2024
Instituto de Salud Carlos III
2016-2024
Instituto Murciano de Investigación Biosanitaria
2020-2023
Centro de Investigación Biomédica en Red
2019-2021
Royal College of Surgeons in Ireland
2020
San Francisco General Hospital
2020
Non-coding RNAs have been drawing increasing attention in recent years as functional data suggest that they play important roles key cellular processes. N-BLR is a primate-specific long non-coding RNA modulates the epithelial-to-mesenchymal transition, facilitates cell migration, and increases colorectal cancer invasion. We performed multivariate analyses of from two independent cohorts patients show abundance associated with tumor stage, invasion potential, overall patient survival. Through...
Summary There is no diagnostic test for primary immune thrombocytopenia (ITP). Certain microRNAs have shown to potential in ITP. We validated 12 identified from two previous studies find a biomarker. The study included ITP cohorts ( n = 61) and healthy controls 28). first cohort involved 24 patients the Prolong study, with newly diagnosed/persistent (<1 year) treated corticosteroids ± IVIG but relapsed/failed respond. second comprised 37 biobank, Østfold Hospital, Norway, had different...
Blood microRNA (miRNA) levels have been associated with and shown to participate in disease pathophysiology. However, the hematopoietic cell of origin blood miRNAs individual miRNA profiles are poorly understood. We report content highly purified normal cells from same individuals. Although T-cells, B-cells granulocytes had highest per cell, erythrocytes contributed more cellular blood, followed by platelets. profiling revealed different patterns expression specific for each lineage....
Understanding the underlying mechanisms of well-substantiated platelet hyporeactivity in neonates is interest given their implications for clinical management newborns, a population at higher bleeding risk than adults (especially sick and preterm infants), as well gaining insight into regulatory biology. Transcriptome analysis useful identifying mRNA signatures affecting function. However, human fetal/neonatal transcriptome has never before been reported. We have used expression array first...
Exosomes are extracellular vesicles that contain nucleic acids, lipids and metabolites, play a critical role in health disease as mediators of intercellular communication. The majority the blood platelet-derived. Compared to adults, neonatal platelets hyporeactive show impaired granule release, associated with defects Soluble N-ethylmaleimide-sensitive fusion Attachment protein REceptor (SNARE) proteins. Since these proteins participate biogenesis exosomes, we investigated potential...
High levels of factor XI (FXI) increase the risk thromboembolic disease. However, genetic and environmental factors regulating FXI expression are still largely unknown. The aim our study was to evaluate regulation by microRNAs (miRNAs) in human liver. In silico prediction yielded four miRNA candidates that might regulate expression. HepG2 cells were transfected with miR-181a-5p, miR-23a-3p, miR-16-5p miR-195-5p. We used mir-494, which not predicted bind F11, as a negative control. Only...
Neonatal platelets are hypo-reactive to the tyrosine kinase-linked receptor agonist collagen. Here, we have investigated whether hypo-responsiveness is related altered levels of glycoprotein VI (GPVI) and integrin α2β1, or defects in downstream signalling events by comparison platelet activation C-type lectin-like 2 (CLEC-2). GPVI CLEC-2 activate a Src- Syk-dependent pathway upstream phospholipase C (PLC) γ2. Phosphorylation conserved YxxL sequence known as (hemi)...
MicroRNAs (miRNAs) are small non-coding RNAs involved in the regulation of gene expression. Dysregulated expression several miRNAs has been found primary immune thrombocytopenia (ITP) suggesting that likely pathogenesis ITP. We aimed to explore differential patients with ITP before and after starting treatment thrombopoietin-receptor agonists (TPO-RAs) clarify their roles pathophysiology ITP, as potential diagnostic prognostic markers this disorder.We performed a profiling study where 179...
MiRNAs have been reported as CIS-acting elements of several hemostatic factors, however, their mechanism TRANS-acting mediated by a transcription factor is little known and could important effects. HNF4α has direct role in the regulation multiple hepatic coagulation genes. Previous vitro studies demonstrated that miR-24-3p miR-34a-5p regulate HNF4A expression. Here we aimed to investigate molecular mechanisms miR-24 miR-34a on through HNF4A. Transfections with HepG2 cells decreased not only...
Chronic myelomonocytic leukemia (CMML) is frequently associated with mutations in the rat sarcoma gene (RAS), leading to worse prognosis. RAS result active RAS-GTP proteins, favoring myeloid cell proliferation and survival inducing NLRP3 inflammasome together apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), which promote caspase-1 activation interleukin (IL)-1β release. Here, we report, cohort of CMML patients KRAS, constitutive monocytes, evidenced by...
Structural variations (SVs) are common in haematological neoplasms.1 Although most SVs have canonical breakpoints, virtually all atypical rearrangements that can be of difficult diagnosis. High-throughput sequencing (HTS) has improved the study SVs, complementing cytogenetics. However, short reads (<150 bp) generated by HTS might lead to inaccurate mapping.2 This may not suppose a mere technical pitfall, but problem faced, guarantee monitoring and guided-treatment patients bearing when...
Abstract Emerging evidence shows the crucial role of inflammation (particularly NF‐κB pathway) in development and progression myelofibrosis (MF), becoming a promising therapeutic target. Furthermore, tailoring treatment with currently available JAK inhibitors (such as ruxolitinib or fedratinib) does not modify natural history disease has important limitations, including cytopenias. Since recent studies have highlighted miR‐146a, negative regulator pathway, pathogenesis MF; here we used...
Abstract The increased risk of cardiovascular events in older men is multifactorial, but the significant reduction testosterone levels has been involved. As this hormone regulates expression TFPI by unknown mechanisms, we aimed to evaluate role miRNAs regulation TFPIα under normal conditions and response androgens. In silico studies allowed selection 4 as potential regulators. Only miR-27a/b-3p significantly reduced two endothelial cell lines. Luciferase assays demonstrated a direct...
Abstract Neonatal platelets are hyporeactive and show impaired agonist-induced secretion despite no obvious abnormalities in their granules. Here, we examined, for the first time, ultrastructure of neonatal adult following agonist activation. Under resting conditions, appeared ultrastructurally identical. Following stimulation, however, noticeable degranulation occurred platelets, while granules remained clearly visible apparently unable to centralize or fuse. To investigate underlying...
Peripheral expansion of cytotoxic T lymphocytes (CTL) derived from the graft in initial stages allogeneic haematopoietic stem cell transplantation (alloHSCT) immune recovery is a well-known physiological event. The description symptomatic large granular lymphocyte leukaemia this setting may generate uncertainty, mostly those cases which CTL (CTLe) persists beyond early period. We aimed to assess nature CTLe during post-alloHSCT period 154 adult patients with long-term surveillance. studied...
The most frequent BCR-ABL1-p210 transcripts in chronic myeloid leukemia (CML) are e14a2 and e13a2. Imatinib (IM) is the common first-line tyrosine–kinase inhibitor (TKI) used to treat CML. Some studies suggest that BCR-ABL1 transcript types confer different responses IM. objective of this study was correlate expression or e13a2 clinical characteristics, cumulative cytogenetic molecular IM, acquisition deep response (DMR) its duration (sDMR), progression rate (CIP), overall survival (OS),...
Summary Micro RNA s have been recognized as critical regulators of gene expression and might affect the risk venous thrombosis. We aimed to identify 3′ untranslated region ( UTR ) variants in coagulation genes that influence factor levels thrombosis risk. The were sequenced subjects with extremely high or low plasma these factors two case‐control studies. In total, 28 identified. Five single nucleotide polymorphisms SNP s) predominantly present one extreme level group F2 rs1799963, F8...