A5064488451- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
- Pluripotent Stem Cells Research
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Synthesis and biological activity
- Complex Systems and Decision Making
- Metal complexes synthesis and properties
- Chemokine receptors and signaling
- Gastrointestinal disorders and treatments
- Biomedical Ethics and Regulation
- Neuroethics, Human Enhancement, Biomedical Innovations
- Ion channel regulation and function
- Phytochemicals and Antioxidant Activities
- Cardiac Ischemia and Reperfusion
- Force Microscopy Techniques and Applications
- Ethics and Legal Issues in Pediatric Healthcare
- Skin Protection and Aging
- Hippo pathway signaling and YAP/TAZ
- Protein Kinase Regulation and GTPase Signaling
- Nuclear Structure and Function
- Animal testing and alternatives
- Crystallization and Solubility Studies
- Meta-analysis and systematic reviews
Institutul National Victor Babes
2006-2020
Carol Davila University of Medicine and Pharmacy
2008-2019
University of Bucharest
2011-2019
Western University
2003-2005
Robarts Clinical Trials
2003-2004
Telocytes (TC) are cells with telopodes (Tp), very long prolongations (up to 100 μm) an uneven caliber ( www.telocytes.com ). Factors determining the dynamics of cellular still unknown, although previous studies showed telopode motility in TC cultures. We comparatively investigated, by time-lapse videomicroscopy, Tp mouse heart seeded on collagen, fibronectin, and laminin. Under our experimental conditions, fibroblasts (cell line L929) behaved differently terms adherence, spreading,...
Summary The atomic force microscope (AFM) has provided nanoscale analyses of surfaces cells that exhibit strong adhesive and cell spreading properties. However, it is frequently reported prior fixation required for reliable imaging with lower In the present study, AFM used to assess effects by glutaraldehyde on elastic modulus a human rhabdomyosarcoma transfectant line RDX2C2. Our results show sharp increase in even mild (0.5% 60 s), accompanied dramatic improvement reproducibility. An...
It is known that beta1 integrins mediate the migratory response of cells to chemokine stimulation. Also, both and chemokines have roles in tumor development. In present study, integrin-chemokine axis assessed using human osteosarcoma (HOS) transfectant expressing CXCR4 receptor for SDF-1 (CXCL12). We first identified vitro specific mediated Results showed on collagen type I laminin, chemotactic was predominantly by alpha2beta1 integrin. On fibronectin, SDF-1-stimulated chemotaxis involved...
There is a strong drive worldwide to discover and exploit the therapeutic potential of large variety plants. In this work, an alcoholic extract Helleborus purpurascens (family Ranunculaceae) was investigated for identification amino acids peptides with putative antiproliferative effects. our separation strategy developed using solvents different polarity in order obtain active compounds. Biochemical components were characterized through spectroscopic (mass spectroscopy) chromatographic...
The ability of cells to undergo shape changes is essential for diverse cellular functions including cell growth, differentiation, and movement. present study examines how an integration the function alpha2beta1 integrin with that receptor epidermal growth factor (EGFR) modulates EGF-stimulated morphological in human rhabdomyosarcoma RD transfectant cells. Upon EGF stimulation, lacked expression (RDpF) underwent contraction; contrast, on (RDX2C2) resulted transient spreading. Integrin alpha2...
We provide evidence that interstitial Cajal-like cells, previously described in human pancreas - pICC (J Cel Mol Med, 9: 169, 2005), are positive for c-kit irrespective of immunohistochemical procedures used. Various sample types (fresh cryosections or formalin-fixed, paraffin-embedded specimens), various slide pretreatments (with without heat-induced epitope retrieval) different antibodies used (Dako polyclonal Santa Cruz monoclonal), all showed CD117-positive pICC.
The growing interest in scientometry stems from ethical concerns related to the proper evaluation of scientific contributions an author working a hard science. In absence consensus, institutions may use arbitrary methods for evaluating scientists employment and promotion. There are several indices that attempt establish most appropriate suggestive position any scientist field he/she works in. A scientist's Hirsch-index (h-index) quantifies their total effective published output, but h-index...
UVA radiation induces multiple and complex changes in the skin, affecting epidermal cell behavior. This study reports effects of exposure on normal (HaCaT) dysplastic (DOK) keratinocytes. The adherence, spreading proliferation were investigated by time-lapse measurement layer impedance different matrix proteins. Prior to exposure, time required for adherence did not differ significantly HaCaT DOK cells, while areas larger cells. Under cells behavior differed terms movement proliferation....
Abstract Sinonasal‐inverted papilloma is a benign tumour with high rate of recurrence, but possible malignant transformation. Therefore, inves tigation predisposition to transformation sinonasal‐inverted gives clinicians the opportunity for adequate trea ment. Topoisomerase II‐α (topoII‐α) and Ki67 are markers cell proliferation in both normal neoplastic tissues its level o expression could be used as predictive parameter. Our goal was investigate by immunochemistry topoII‐in inverted...
UVA affects epidermal cell physiology in a complex manner, but the harmful effects have been studied mainly terms of DNA damage, mutagenesis and carcinogenesis. We investigated on membrane integrity antioxidant defense dysplastic keratinocytes after one two hours irradiation, both immediately exposure, 24 h post-irradiation. To determine oxidative stress membrane, lipid peroxidation was correlated with changes fatty acid levels. Membrane permeability were assessed by propidium iodide...
Abstract The diagnosis of uterine smooth muscle neoplasms by light microscopy is difficult. Multiple classification schemes have been proposed based on mitotic rate, nuclear atypia, and the presence or absence necrosis. None these systems has entirely successful. This study was undertaken to evaluate use selected immunohistochemical histochemical markers in differentiating tumors, addition accepted morphologic criteria. Ten cases each following: leiomyosarcomas (LMS), atypical leiomyomas...
No progress can ever be made without risk-taking. The discovery of stem cells and their improved handling brought new perspectives in tissue engineering. Nonetheless, whatever the method used to obtain (embryonic, from embryos got naturally, or by vitro fertilization, produced somatic cell nuclear transfer, inducing pluripotent cells, supplied harvesting adult cells), use therapy, even through engineered products, remains questionable due anticipated unexpected risks. Both professionals...