A5041462448- PARP inhibition in cancer therapy
- Toxin Mechanisms and Immunotoxins
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- Calcium signaling and nucleotide metabolism
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Dermatology and Skin Diseases
- NF-κB Signaling Pathways
- RNA Research and Splicing
- Psoriasis: Treatment and Pathogenesis
- Sirtuins and Resveratrol in Medicine
- RNA modifications and cancer
- Cytokine Signaling Pathways and Interactions
- Autophagy in Disease and Therapy
- Enzyme Production and Characterization
- Nuclear Structure and Function
- Animal Genetics and Reproduction
- Chronic Lymphocytic Leukemia Research
- RNA and protein synthesis mechanisms
RWTH Aachen University
2016-2025
Universitätsklinikum Aachen
2001-2017
Universitäts Frauenklinik
2017
ETH Zurich
2009
Heinrich Heine University Düsseldorf
2006
Biologie Labor
2005
Institut für Molekularbiologie und Analytik (Germany)
2002
Medizinische Hochschule Hannover
1992-2001
Uppsala University Hospital
1999
Fred Hutch Cancer Center
1986-1992
Max is a helix-loop-helix zipper protein that associates in vitro with Myc family proteins to form sequence-specific DNA-binding complex. We show here, by means of coimmunoprecipitation assay anti-Myc and anti-Max antibodies, are associated vivo essentially all the newly synthesized can be detected complex Max. This possesses specific activity for CACGTG-containing oligonucleotides. Although itself highly stable protein, rapidly degraded during or after its association In shown nuclear...
NF-κB regulates the expression of a large number target genes involved in immune and inflammatory response, apoptosis, cell proliferation, differentiation survival. In this study, we identified SIRT2 as deacetylase transcription factor p65. is member family sirtuins, which are NAD+-dependent deacetylases several cellular processes. interacts with p65 cytoplasm deacetylates vitro vivo at Lys310. Moreover, hyperacetylated Lys310 Sirt2−/− cells after TNFα stimulation, results increase subset...
Silent information regulator 1 (SIRT1) represents an NAD + -dependent deacetylase that inhibits proapoptotic factors including p53. Here we determined whether SIRT1 is downstream of the prototypic c- MYC oncogene, which activated in majority tumors. Elevated expression c-MYC human colorectal cancer correlated with increased protein levels. Activation a conditional allele induced levels protein, , and nicotinamide-phosphoribosyltransferase ( NAMPT ) mRNA several cell types. This increase...
ADP‐ribosylation, a modification of proteins, nucleic acids, and metabolites, confers broad functions, including roles in stress responses elicited, for example, by DNA damage viral infection is involved intra‐ extracellular signaling, chromatin transcriptional regulation, protein biosynthesis, cell death. ADP‐ribosylation catalyzed ADP‐ribosyltransferases (ARTs), which transfer ADP‐ribose from NAD + onto substrates. The modification, occurs as mono‐ or poly‐ADP‐ribosylation, reversible due...
Myc oncoproteins promote cell cycle progression in part through the transcriptional up-regulation of cyclin D2 gene. We now show that is bound to promoter vivo. Binding induces expression and histone acetylation at a single nucleosome MycBoxII/TRRAP-dependent manner. Down-regulation mRNA differentiating HL60 cells preceded by switch occupancy from Myc/Max Mad/Max complexes, loss TRRAP binding, increased HDAC1 deacetylation. Thus, recruitment regulation are critical for activation Myc.
The murine neutrophil elastase (NE) gene is expressed specifically in immature myeloid cells. A 91-bp NE promoter region contains three cis elements which are conserved evolutionarily and essential for activation of the differentiating 32D cl3 These bound c-Myb (at -49), C/EBPalpha -57), PU.1 -82). In NIH 3T3 cells, was activated by c-Myb, C/EBPalpha, PU.1, via their respective binding sites. Cooperative seen any combination including all together, again DNA-binding CV-1 but not cooperation...
Cyclin-dependent kinases (Cdks) fulfill key functions in many cellular processes, including cell cycle progression and cytoskeletal dynamics. A limited number of Cdk substrates have been identified with few demonstrated to be regulated by Cdk-dependent phosphorylation. We identify on protein expression arrays novel cyclin E–Cdk2 substrates, SIRT2, a member the Sirtuin family NAD+-dependent deacetylases that targets α-tubulin. define Ser-331 as site phosphorylated E–Cdk2, A–Cdk2, p35–Cdk5...