A5033513688- Calcium Carbonate Crystallization and Inhibition
- Bone Tissue Engineering Materials
- Marine Bivalve and Aquaculture Studies
- Molecular Biology Techniques and Applications
- Plant Molecular Biology Research
- Historical and Literary Studies
- Ancient and Medieval Archaeology Studies
- Marine Biology and Environmental Chemistry
- Plant Pathogens and Fungal Diseases
- Plant nutrient uptake and metabolism
- Marine Ecology and Invasive Species
- Gene expression and cancer classification
- Leprosy Research and Treatment
- Fungal Biology and Applications
- Mycobacterium research and diagnosis
- MicroRNA in disease regulation
- Evolution and Paleontology Studies
- Advanced biosensing and bioanalysis techniques
- Plant-Microbe Interactions and Immunity
- Death, Funerary Practices, and Mourning
- Ancient Mediterranean Archaeology and History
- Nail Diseases and Treatments
- Bone and Dental Protein Studies
- Aluminum toxicity and tolerance in plants and animals
- Historical and Literary Analyses
Université de Bourgogne
2008-2024
Biogéosciences
2010-2024
Centre National de la Recherche Scientifique
2008-2024
École Pratique des Hautes Études
2022-2024
University of Lausanne
2008-2015
University Hospital of Lausanne
2003
Cold Spring Harbor Laboratory
1999
Palo Alto Institute
1924
Ants have evolved very complex societies and are key ecosystem members. Some ants, such as the fire ant Solenopsis invicta , also major pests. Here, we present a draft genome of S. assembled from Roche 454 Illumina sequencing reads obtained focal haploid male his brothers. We used comparative genomic methods to obtain insight into unique features genome. For example, found that this harbors four adjacent copies vitellogenin. A phylogenetic analysis revealed an ancestral vitellogenin gene...
In higher plants, seed development requires maternal gene activity in the haploid (gametophytic) as well diploid (sporophytic) tissues of developing ovule. The Arabidopsis thaliana MEDEA (MEA) encodes a SET-domain protein Polycomb group that regulates cell proliferation by exerting gametophytic control during development. Seeds derived from female gametocytes (embryo sacs) carrying mutant mea allele abort and exhibit defects both embryo endosperm. this study we show mutation affects an...
Profiling miRNA levels in cells with microarrays is becoming a widely used technique. Although normalization methods for mRNA gene expression arrays are well established, array has so far not been investigated detail. In this study we investigate the impact of on data generated Agilent platform. We have developed method to select nonchanging miRNAs (invariants) and use them compute linear regression coefficients or variance stabilizing (VSN) parameters. compared invariants by scaling,...
Profiling microRNA (miRNA) expression is of widespread interest given the critical role miRNAs in many cellular functions. can be achieved via hybridization-based (microarrays), sequencing-based, or amplification-based (quantitative reverse transcription-PCR, qPCR) technologies. Among these, microarrays face significant challenge accurately distinguishing between mature and immature miRNA forms, different vendors have developed methods to meet this challenge. Here we measure differential...
Dermatophytes are highly specialized filamentous fungi which cause the majority of superficial mycoses in humans and animals. The high secreted proteolytic activity these microorganisms during growth on proteins is assumed to be linked their particular ability exclusively infect keratinized host structures such as skin stratum corneum, hair, nails. Individual dermatophyte proteases were recently described with vitro digestion keratin. However, overall adaptation transcriptional response...
The priming agent β-aminobutyric acid (BABA) is known to enhance Arabidopsis resistance the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 by potentiating salicylic (SA) defence signalling, notably PR1 expression. molecular mechanisms underlying this phenomenon remain unknown. A genome-wide microarray analysis of BABA during Pst infection revealed direct and primed up-regulation genes that are responsive SA, SA analogue benzothiadiazole pathogens. In addition, was found...
The extensive use of mollusc shell as a versatile raw material is testament to its importance in prehistoric times. consistent choice certain species for different purposes, including the making ornaments, direct representation how humans viewed and exploited their environment. necessary taxonomic information, however, often impossible obtain from objects that are small, heavily worked or degraded. Here we propose novel biogeochemical approach track biological origin shell. We conducted an...
Approximately 0.2 % of all angiosperms are classified as metal hyperaccumulators based on their extraordinarily high leaf contents, for example >1 zinc, >0.1 nickel or >0.01 cadmium (Cd) in dry biomass. So far, hyperaccumulation has been considered to be a taxon-wide, constitutively expressed trait, the extent which depends solely available concentrations soil. Here we show that facultative metallophyte Arabidopsis halleri, both insect herbivory and mechanical wounding leaves trigger an...
Identification of small polymorphisms from next generation sequencing short read data is relatively easy, but detection larger deletions less straightforward. Here, we analyzed four divergent Arabidopsis accessions and found that intersection absent coverage with weak tiling array hybridization signal reliably flags deletions. Interestingly, individual were frequently observed in two or more the examined, suggesting variation gene content partly reflects a common history deletion events.
Stable gene silencing by RNA interference (RNAi) can be achieved expression of small hairpin RNAs (shRNAs) from polymerase III promoters. We have tested lentiviral vectors expressing shRNAs targetting CCR5 in primary CD4 T cells donors representing various and CCR2 genetic backgrounds covering the full spectrum levels permissiveness for HIV-1 infection. A linear decrease resulted a logarithmic cellular infection, giving up to three logs protection infection vitro. Protection was maintained...
The recently released Affymetrix Human Gene 1.0 ST array has two major differences compared with standard 3' based arrays: (i) it interrogates the entire mRNA transcript, and (ii) uses DNA targets. To assess impact of these on performance, we performed a series comparative hybridizations between HG-U133 Plus 2.0 Illumina HumanRef-8 BeadChip arrays. Additionally, both RNA targets were hybridized results show that overall reproducibility is best. When looking only at high intensity probes,...
Among Antarctic bryozoans, some species are able to develop calcitic bioconstructions promoting habitat complexity, but the processes leading biomineral formation mostly unknown. The present work investigated three from morphological skeletal features, including organic matrix associated with skeleton (SOM). Cellarinella nutti Rogick, 1956 and Reteporella frigida Waters, 1904 were collected in November 2018 a shallow site (25 m) njegovanae deep (110 at Terra Nova Bay (Ross Sea, Antarctica)....
To mineralize their shells, molluscs secrete a complex cocktail of proteins—collectively defined as the calcifying shell matrix—that remains occluded in exoskeleton. Nowadays, protein extracts from shells are recognized potential source bioactive substances, among which signalling molecules, bactericides or protease inhibitors offer most tangible perspectives applied sciences, health, and aquaculture. However, one technical obstacle testing activity lies high insolubility. In this paper, we...
Empreintes de crocodylomorphes et dinosaures du Jurassique inférieur Veillon (ouest la France): révision ichnotaxonomique matériel type (Collection Lapparent). Depuis seconde moitié XXe siècle, le littoral France) est devenu un site capital pour l'étude des traces pas d'archosaures d'Europe. Parmi millier d'empreintes découvertes dans ce secteur, certaines historiques sont devenues références ichnotaxonomiques nombreuses études comparatives. Cependant, n'a jamais été révisé. Ici, nous...
French Polynesia is renowned for the production of Tahitian black pearl. These gems are obtained by grafting a nucleus into gonad receiving oyster together with graft, i.e. small section mantle tissue donor oyster. This procedure initiates formation pearl sack around nucleus, and subsequently, deposition concentric layers nacre. The plays key-role in its characteristics influence markedly quality final product. As it manufactured from mollusc shells, contains percentage organics. In present...
À partir de l’exemple du programme Trans’Tyfipal®, mené par le laboratoire Biogéosciences l’université Bourgogne et qui a pour objectif d’inventorier tous les spécimens types paléontologiques conservés en France, l’auteur montre comment l’un des grands défis musées universitaires est se développer bonne harmonie entre la recherche, formation valorisation.
We have identified a novel shell protein, accripin11, as major soluble component of the calcitic prisms fan mussel Pinna nobilis. Initially retrieved from cDNA library, its full sequence is confirmed here by transcriptomic and proteomic approaches. The mature protein 103 residues with theoretical molecular weight 11 kDa moderately acidic (pI 6.74) except for C-terminus which highly enriched in aspartic acid. exhibits peculiar cysteine pattern central domain. shares similarity six other...