A5037470884- Nail Diseases and Treatments
- Plant Pathogens and Fungal Diseases
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Yeasts and Rust Fungi Studies
- Dermatology and Skin Diseases
- Fungal Biology and Applications
- Infectious Diseases and Mycology
- Plant Disease Resistance and Genetics
- Mycorrhizal Fungi and Plant Interactions
- Sarcoma Diagnosis and Treatment
- Microbial Natural Products and Biosynthesis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Bee Products Chemical Analysis
- Fungal and yeast genetics research
- Allergic Rhinitis and Sensitization
- Retinal and Optic Conditions
- Bone Tumor Diagnosis and Treatments
- Fermentation and Sensory Analysis
- Mycobacterium research and diagnosis
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Dermatological diseases and infestations
- Plant Pathogens and Resistance
Meiji Pharmaceutical University
2016-2025
Teikyo University
2002-2022
Juntendo University
2017
Kure Medical Center
2011-2015
National Hospital Organization
2011-2015
Hiroshima Prefectural Hospital
2004-2012
Kindai University
2010
Cambridge University Press
2010
New York University Press
2010
Hiroshima University
1984-2009
ABSTRACT Lipophilic yeasts of the genus Malassezia are part normal cutaneous microflora and considered one factors that trigger atopic dermatitis (AD). We isolated two strains from a healthy Japanese female. Analysis D1/D2 26S ribosomal DNA internal transcribed spacer region sequences isolates suggested they new members . propose name japonica sp. nov. for isolates. M is easily distinguished seven known lipophilic species by its ability to assimilate Tween 40 60 inability 20 80 grow at 40°C....
Malassezia species are considered to be one of the exacerbating factors in atopic dermatitis (AD). During examination cutaneous colonization AD patients, we found a new on surface patients' skin. Analysis ribosomal DNA sequences suggested that isolates belonged genus MALASSEZIA: They did not grow Sabouraud dextrose agar but utilized specific concentrations Tween 20, 40, 60, and 80 as lipid source. Thus, concluded our were members propose name dermatis sp. nov. for these isolates.
ABSTRACT The nucleotide sequences of the internal transcribed spacer (ITS) 1 and 2 regions in rRNA gene were determined by directly sequencing PCR-amplified fragments for all species (17 five varieties) genus Trichosporon . Comparative sequence analysis suggests that six medically relevant species, T. asahii , asteroides cutaneum inkin mucoides ovoides can be readily identified their ITS sequences. In addition, showed conspecific strains have fewer than 1% differences overall. Molecular...
ABSTRACT We determined the sequence of intergenic spacer (IGS) 1 region, which is located between 26S and 5S rRNA genes, in 25 species genus Trichosporon . IGS sequences varied length from 195 to 719 bp. Comparative analysis suggested that divergence has been greater than internal transcribed regions. also identified five genotypes T asahii , a major causative agent deep-seated trichosporonosis, based on 43 strains. Most isolates originated Japan were genotype 1, whereas American 3 or 5. Our...
Abstract Over the last few years, new Malassezia species have been found regularly in Japanese subjects. We isolated another from a patient with seborrheic dermatitis (SD), and named it M. yamatoensis . In its physiological characteristics utilization of Tween by is similar to that furfur dermatis It distinguished growth temperature. To examine distribution microorganism skin patients SD atopic (AD), healthy subjects, we applied transparent dressings skin, detected DNA using...
Patients with atopic dermatitis (AD) are highly susceptible to viral, bacterial, and fungal skin infections because their is dry this compromises the barrier function of skin. Therefore, microbiota patients AD believed be different from that healthy individuals. In present study, nine mild, moderate, or severe ten subjects were compared using an rRNA clone library. Fungal D1/D2 large subunit analysis 3647 clones identified 58 species seven unknown phylotypes in face scale samples subjects....
Cryptococcosis is a major fungal disease caused by members of the Cryptococcus gattii and neoformans species complexes. After more than 15 years molecular genetic phenotypic studies much debate, proposal for taxonomic revision was made.
Abstract The skin surface is colonized by a wide variety of fungi and bacteria. While many these organisms, including Malassezia , Candida Streptococcus Staphylococcus species, are associated with provocation and/or exacerbation psoriasis, detailed analysis the cutaneous fungal microbiome in psoriatic patients has yet to be performed. To identify disease‐specific microbiota on scale samples, r RNA gene sequences from 12 healthy controls were analyzed pyrosequencing. A total 317 806...
ABSTRACT Members of the genus Malassezia , lipophilic yeasts, are considered to be one exacerbating factors in atopic dermatitis (AD). We examined variation cutaneous colonization by species AD patients and compared it with healthy subjects. Samples were collected applying transparent dressings skin lesions patients. DNA was extracted directly from amplified a specific nested PCR assay. -specific detected all samples obtained 32 In particular, globosa M. restricta approximately 90% furfur...
The prognostic value of the time identification lung metastasis was investigated in 280 patients with metastatic osteosarcoma as a multi-institutional study Japanese Musculoskeletal Oncology Group.The were divided into four groups: group 1, identified at initial presentation; 2, those during preoperative chemotherapy; 3, postoperative chemotherapy, and 4, after completion treatment. Survivals groups compared. Additionally, effects number nodules, metastasectomy, effect chemotherapy on...
Although active targeting of anticancer drugs using magnetically responsive carriers is a very attractive treatment approach for solid tumors, successful results are limited. In particular, the therapeutic utility intravenously administered has to date not been clearly established. The present study investigates magnetic liposomes designed act as drug carriers, which can be effectively delivered tumors via intravenous administration. Magnetic with incorporated adriamycin (magnetic ADR...
The development of an active drug delivery system is attractive approach to increase the targetability anticancer agents. In present study, we examined efficiency systemic chemotherapy with small magnetic liposomes containing doxorubicin (magnetic DOX liposomes) and externally applied electromagnetic force in osteosarcoma-bearing hamsters. Syrian male hamsters inoculated osteosarcoma, OS515, limb were studied 7 days after inoculation. this was evaluated by measuring tissue distribution...
The lipophilic yeast Malassezia is an exacerbating factor in atopic dermatitis (AD) and colonizes the skin surface of patients with AD. With goal reducing number cells, we investigated antifungal activities a therapeutic agent for AD, tacrolimus, azole agents itraconazole ketoconazole against species vitro. We examined 125 strains 11 currently accepted by using agar dilution method. All were very susceptible to both agents, MICs ranging from 0.016 0.25 mug/ml. Tacrolimus had half strains, 16...
Monocrotaline induces microvascular leak and pulmonary hypertension in rats. We have hypothesized that the is related some way to precedes it. In rats given 40 mg monocrotaline/kg body wt subcutaneously, lung wet weight-to-dry weight ratios albumin content began increase within first 3 days became maximal at 1 wk. Alveolar lavage fluid showed little or no protein. Right ventricular hypertrophy increased progressively from 2 through An dry paralleled right hypertrophy. The amount of blood...
In the present study, we evaluated safety and effectiveness of SYT-SSX-derived peptide vaccines in patients with advanced synovial sarcoma. A 9-mer spanning SYT-SSX fusion region (B peptide) its HLA-A*2402 anchor substitute (K9I) were synthesized. Protocols A1 A2, alone administered subcutaneously six times at 14-day intervals. The B was used Protocol A1, whereas K9I A2. B1 B2, mixed incomplete Freund's adjuvant then addition, interferon-α injected on same day again 3 days after vaccination....
Skin surface colonization starts after birth. It is thought that early microbial affects the development of skin immune functions. Although Malassezia predominant fungus in microbiota healthy individuals, microorganism associated with atopic dermatitis and seborrheic dermatitis. In present study, transmission from mothers to their neonates was elucidated using as an indicator.Temporal changes level 27 were investigated by real-time polymerase chain reaction assay. The genotypes colonizing...
Four new 4-hydroxy-2-pyridone alkaloids, didymellamides A–D (1–4), were isolated from the marine-derived fungus Stagonosporopsis cucurbitacearum. The structures of 1–4 elucidated spectroscopic data (NMR, MS, and IR), absolute configuration 1 was determined by X-ray diffraction analysis. Didymellamide A (1) exhibited antifungal activity against azole-resistant Candida albicans.
Seborrheic dermatitis (SD) is a chronic inflammatory dermatologic condition in which erythema and itching develop on areas of the body with sebaceous glands, such as scalp, face chest. The inflammation evoked directly by oleic acid, hydrolyzed from sebum lipases secreted skin microorganisms. Although fungal genus, Malassezia, thought to be causative agent SD, analysis bacterial microbiota samples patients SD necessary clarify any association Malassezia because comprises diverse genera. In...
Starch is considered a potential feedstock for biofuel production, particularly in light of the large-scale landfilling food waste and other starchy materials worldwide. Lipid accumulation by oleaginous yeast promising method biodiesel production from starch. However, most yeasts are grown on monosaccharides or oligosaccharides because they cannot directly utilize We therefore investigated starch-assimilation ability 1,200 yeasts. found that Cryptococcus terricola could be used fuel through...