A5021347759- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
- Antifungal resistance and susceptibility
- Yeasts and Rust Fungi Studies
- Phytoplasmas and Hemiptera pathogens
- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- Bacterial Identification and Susceptibility Testing
- Lichen and fungal ecology
- Infectious Diseases and Mycology
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Fungal Plant Pathogen Control
- Mycorrhizal Fungi and Plant Interactions
University of Phayao
2011-2021
Westerdijk Fungal Biodiversity Institute
2011-2018
University of Amsterdam
2018
Canisius-Wilhelmina Ziekenhuis
2016
University Medical Center Utrecht
2012-2013
Utrecht University
2013
Naresuan University
2011
Cryptococcosis is a major fungal disease caused by members of the Cryptococcus gattii and neoformans species complexes. After more than 15 years molecular genetic phenotypic studies much debate, proposal for taxonomic revision was made.
ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was used for an extensive identification study arthroconidial yeasts, using 85 reference strains from the CBS-KNAW yeast collection and 134 clinical isolates collected medical centers in Qatar, Greece, Romania. The test set included 72 ascomycetous yeasts ( Galactomyces , Geotrichum Saprochaete Magnusiomyces spp.) 147 basidiomycetous Trichosporon Guehomyces spp.). With minimal preparation...
Cryptococcosis is an important fungal disease in Asia with estimated 140,000 new infections annually the majority of which occurs patients suffering from HIV/AIDS. Cryptococcus neoformans variety grubii (serotype A) major causative agent this disease. In present study, multilocus sequence typing (MLST) using ISHAM MLST consensus scheme for C. neoformans/C. gattii species complex was used to analyse nucleotide polymorphisms among 476 isolates pathogen obtained 8 Asian countries. Population...
ABSTRACT An interlaboratory study using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) to determine the identification clinically important yeasts ( n = 35) was performed at 11 clinical centers, one company, and reference center Bruker Daltonics MALDI Biotyper system. The optimal cutoff for MALDI-TOF MS score investigated receiver operating characteristic (ROC) curve analyses. percentages correct identifications were compared different sample...
The global burden of HIV-associated cryptococcal meningitis is estimated at nearly one million cases per year, causing up to a third all AIDS-related deaths. Molecular epidemiology constitutes the main methodology for understanding factors underpinning emergence this understudied, yet increasingly important, group pathogenic fungi. Cryptococcus species are notable in degree that virulence differs amongst lineages, and highly-virulent emerging lineages changing patterns human disease both...
Infections caused by Malassezia yeasts are most likely underdiagnosed, because fatty acid supplementation is needed for growth. Rapid identification of species essential appropriate treatment Malassezia-related skin infections, fungaemia and nosocomial outbreaks in neonates, children adults can be life-saving those patients. Ma-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) has been reported to a rapid reliable diagnostic tool identify clinically important...
Background Cryptococcus neoformans is a pathogenic yeast that causes cryptococcosis, life threatening disease. The prevalence of cryptococcosis in Asia has been rising after the onset AIDS epidemic and estimates indicate more than 120 cases per 1,000 HIV-infected individuals year. Almost all cryptococcal disease both immunocompromised immunocompetent patients are caused by C. var. grubii. Epidemiological studies on pan-Asia have not reported. present work genetic diversity fungus...
Summary Isolation of representatives the Cryptococcus neoformans / gattii species complex can be made using dopamine containing media, such as Niger seed agar and l ‐ DOPA agar. Here, we describe an alternative medium that uses banana flowers. Banana is a fruit widely available in tropical subtropical countries have high numbers cryptococcosis patients. This blossom‐based useful for enrichment isolates C . from environmental clinical materials. The blossom ( BABA ) with without creatinine...
We had determined that 190 Cryptococcus neoformans isolates (104 clinical and 86 environmental isolates from northern Thailand) were all susceptible to amphotericin B, fluconazole, itraconazole ketoconazole. Of 14 selected isolates, one isolate (CN4969) exhibited heterogeneity in fluconazole resistance, which produced two subpopulations of the fluconazole-resistant (CN4969HR) -susceptible (CN4969S) subpopulations. To clarify molecular mechanisms expression drug resistance genes was by using...
It is well-known that Aspergillus terreus harbors higher MIC for amphotericin B and in the past couple of years body evidence showing azole-resistant spp. increasing.Clinicians are treating more high-risk populations, prophylactic therapy clinical practice has a growing impact influencing epidemiological data.In Candida disease there seems to be shift from albicans non-albicans species.In ICUs, patients with infection have nearly seven-fold mortality compared those C. albicans.The global...
Oseltamivir Influenza-associated aspergillosis In vitro experiments:PBMC and PMN were isolated from healthy volunteers istimulated with A. fumigatus conidia, in the presence or absence of neuraminidase oseltamivir.Cytokines, reactive oxygen species (ROS) production, killing activity meaasured.In vivo experiments corticosteroid cyclophosphamide -treated mice infected fumigatus, withour pre-exposure to Oseltamivir.To study role oseltamivir host response against