A5090165109- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Bacterial Identification and Susceptibility Testing
- Building materials and conservation
- Plant Pathogens and Fungal Diseases
- Conservation Techniques and Studies
- 3D Printing in Biomedical Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Algal biology and biofuel production
- Indoor Air Quality and Microbial Exposure
- Antimicrobial Resistance in Staphylococcus
- Cystic Fibrosis Research Advances
- Nail Diseases and Treatments
- Bacterial biofilms and quorum sensing
- Nanoparticles: synthesis and applications
- Toxin Mechanisms and Immunotoxins
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Paranormal Experiences and Beliefs
- Aquaculture disease management and microbiota
- Medical Device Sterilization and Disinfection
- Diphtheria, Corynebacterium, and Tetanus
- Infectious Diseases and Mycology
- Lichen and fungal ecology
- Paleopathology and ancient diseases
University of Ljubljana
2013-2024
Institut za Reumatologiju
2014-2017
Czech Academy of Sciences, Institute of Microbiology
2017
Cryptococcosis is a major fungal disease caused by members of the Cryptococcus gattii and neoformans species complexes. After more than 15 years molecular genetic phenotypic studies much debate, proposal for taxonomic revision was made.
To date, azoles represent the only viable option for oral treatment of invasive Candida infections, while rates azole resistance among non-albicans spp. continue to increase. The objective this sub-analysis European multicenter observational cohort study III was describe demographical and clinical characteristics requiring prolonged hospitalization solely complete intravenous (iv) antifungal (AF Tx).Each participating hospital (number eligible hospitals per country determined by population...
(1) Background: Airborne microbes are an integral part of a cave ecosystem. Cave allochtonous airborne microbiota, which occurs mainly during aerosolization from underground river, animals, and visitors, is particularly pronounced in show caves. The impacts tourists natural river on the air were estimated large spaces within Škocjan Caves; (2) Methods: Simultaneously with measurements atmospheric parameters, cultivable bacteria impacted, counted identified using MALDI-TOF MS (Matrix-Assisted...
Many infectious diseases are transmitted via the air and are, therefore, particularly difficult to combat. These infections include various invasive mycoses caused by molds. The usual route of infection is inhalation conidia. In hospitals, can also occur through deposition conidia in otherwise sterile anatomical sites during surgical other procedures. Therefore, knowledge airborne mold concentrations lead measures protect patients from fungal infections. literature on this topic contains...
A natural cave environment subject to regular human visitation was selected for aerobiological study minimize the effects of severe temperature fluctuations, UV radiation, and desiccation stress on aerobiome. The longer sampling period bioaerosols, up 22 months, generally not associated with a proportionally incremental cumulative increase microbial biomass. culture-independent biomass indicator ATP enabled quick reliable determination total Total airborne influenced by cave, as confirmed...
We studied the efficiency of three culture series microalgae Phaeodactylum tricornutum (P. tricornutum) and bacteria Thalassospira sp. (axenic microalgae, bacterial co-culture two) in removing bisphenols (BPs) from their growth medium. Bacteria were identified by 16S ribosomal RNA polymerase chain reaction (16S rRNA PCR). The microorganism rate was determined flow cytometry. Cultures isolates small cellular particles (SCPs) imaged scanning electron microscopy (SEM) cryogenic transmission...
Speleotherapy is one of the non-pharmacological methods for treatment and rehabilitation patients with chronic respiratory diseases, especially those obstructive pulmonary disease (COPD) asthma. On hand, alleged main advantages speleotherapeutic caves low microbial load in air absence other aeroallergens, but on due to lack comprehensive monitoring, there little information pristine human-influenced aerobiota such environments. The aim this study was assess anthropogenic effects...
Blood culture systems are a potential alternative to classical cultivation of fungi on mycological media, but there limited data the suitability these for culturing other sample types (e.g., sterile body fluids). We conducted prospective study evaluate different blood (BC) bottles detection fungal species in non-blood samples. A total 43 isolates were tested their ability grow BD BACTEC Mycosis-IC/F (Mycosis bottles), Plus Aerobic/F (Aerobic bottles) and Anaerobic/F (Anaerobic (Becton...
Around 85% of the environments on Earth are permanently or seasonally colder than 5 °C. Among those, poles constitute unique biomes, which harbor a broad variety microbial life, including an abundance fungi. Many fungi have outstanding ability to withstand extreme conditions and play vital ecosystem roles decomposers as well obligate facultative symbionts many other organisms. Due their dispersal capabilities, microorganisms from cryosphere samples can be distributed around world. Such...
Abstract Background Candida bloodstream infections (BSI) became an important invasive disease in the late 20th century, particular among immunocompromised patients. Although considerable progress has been made management of patients with mycoses, BSI are still widespread hospitalised and associated relatively high mortality. Objectives We conducted a retrospective study to evaluate patient characteristics, incidence, species distribution antifungal susceptibility isolates spp. as well...
Biofilms of different colours that colonize surfaces within karst caves represent a source nutrients. They occur commonly and abundantly at sites with sediments, close to seepages or underground rivers. Golden-yellow subaerial biofilms are particularly well observed because their contrast surroundings, the characteristics pigment recently, even more, due light-emitting diode (LED) illumination. Yellow microbial were sampled from three in southwestern Slovenia, Dimnice, Križna jama Sveta...
Aerosols in caves are natural tracers and, together with climatic parameters, provide a detailed insight into atmospheric conditions, responses to changes and anthropogenic influences caves. Microbiological air monitoring show is becoming increasingly useful understand cave ecosystems implement review measures for sustainable use tourism development. In 2017 2018, along tourist trails Postojnska jama Škocjanske jame (Slovenia) was sampled before after visits. Samples were analysed using...
Escherichia coli, one of the primary intestinal commensal bacteria in humans and endothermic animals, is commonly considered an indicator faecal pollution. E. coli strains were isolated from karst rivers under different hydrological conditions, footpaths tourist caves bat guano. Isolates tested for phenotypic resistance to ampicillin, chloramphenicol, ciprofloxacin, nalidixic acid, tetracycline trimethoprim. The highest percentage antimicrobial resistant was found waters, followed by those...
The effective identification of bacterial and fungal isolates is essential for microbiological monitoring in environments like speleotherapeutic caves. This study compares MALDI-TOF MS the OmniLog ID System, two high-throughput culture-based methods. identified 80.0% to species level, while System 92.9%. However, species-level matches between methods were only 48.8%, revealing considerable discrepancies. For discrepant results, matched molecular at genus level 90.5% cases, 28.6%,...
The aim of our prospective cohort study was to determine the incidence, genetic relatedness and risk factors for colonization with ampicillin high-level aminoglycoside-resistant enterococci (ARHLARE) among patients hospitalized in intensive care unit. During 15-month period, we included 105 patients. only independent factor ARHLARE days cefotaxime/ceftriaxone therapy [odds ratio (OR): 1·13; 95% confidence interval (CI) 1·10–1·27; P = 0·045]. Patients higher total use antibiotics, on...
In the last two decades prevalence of fungal infections is increasing for various reasons. One them advance medical science and associated longer life expectancy in some patient groups. This includes cystic fibrosis patients who encounter diseases already their childhood. Fungal pathogens isolated high frequencies from respiratory tract include Aspergillus fumigatus, Candida albicans Scedosporium apiospermum. case fibrosis, these organisms normaly colonise intestinal mucosae can cause...
Small cellular particles are released into the surroundings of cells and proposed to play an important role in intercellular communication consequently responses microbial com-munities environmental stressors. We studied connection between small parti-cles efficiency three culture series microalge Phaeodactylum tricornutum bac-teria (axenic microalgae, bacterial co-culture two) removing bisphenols from their growth medium. The microorganism rate was determined by flow cytometry, protein...