A5051271354- Viral Infections and Vectors
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Orthopaedic implants and arthroplasty
- Antibiotic Resistance in Bacteria
- Orthopedic Infections and Treatments
- Acne and Rosacea Treatments and Effects
- Antibiotic Use and Resistance
- Viral Infections and Outbreaks Research
- Streptococcal Infections and Treatments
- Dermatological diseases and infestations
- Total Knee Arthroplasty Outcomes
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Identification and Susceptibility Testing
- Mosquito-borne diseases and control
- Sarcoma Diagnosis and Treatment
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Identification and Quantification in Food
- Escherichia coli research studies
- Oral microbiology and periodontitis research
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Leptospirosis research and findings
University of Ljubljana
2008-2024
Institute of Oncology Ljubljana
2011-2024
Czech Academy of Sciences, Institute of Microbiology
2021
Anaplasma phagocytophilum is a Gram-negative obligate intracellular bacterium that replicates in neutrophils. It transmitted via tick-bite and causes febrile disease humans animals. Human granulocytic anaplasmosis regarded as an emerging infectious North America, Europe Asia. However, although increasingly detected, it still rare Europe. Clinically apparent A. infections animals are mainly found horses, dogs, cats, sheep cattle. Evidence from cross-infection experiments isolates of distinct...
Antimicrobial resistance of Escherichia coli is a growing problem in both developed and developing countries. This study aimed to investigate the phenotypic antimicrobial E. isolates (n = 260) isolated from stool specimen patients attending public health facilities Addis Ababa Hossana. also characterize phenotypically confirmed extended-spectrum beta-lactamase (ESBL)-producing 22) using whole-genome sequencing. Resistance 18 different antimicrobials was assessed disc diffusion method...
Whole-genome sequencing (WGS) has replaced Pulsed-field gel electrophoresis (PFGE)-based bacterial genotyping as the reference method. We investigated suitability of purified genomic DNA extracted from PFGE agarose plugs stored in a laboratory collection for WGS cases where isolates are no longer available. Our study shown that can be successfully performed on plugs.
Abstract Background Anaplasma phagocytophilum infects a wide variety of hosts and causes granulocytic anaplasmosis in humans, horses dogs tick-borne fever ruminants. Infection with A. results the modification host gene expression immune response. The objective this research was to characterize pigs ( Sus scrofa ) naturally experimentally infected trying identify mechanisms that help explain low infection prevalence species. Results For analysis pigs, microarray hybridization used....
Hemorrhagic fever with renal syndrome (HFRS) and Crimean-Congo hemorrhagic (CCHF) are important viral fevers (VHF), especially in the Balkan region. Infections Dobrava or Puumala orthohantavirus orthonairovirus can vary from a mild, nonspecific febrile illness, to severe disease fatal outcome. The pathogenesis of both diseases is poorly understood, but it has been suggested that host’s immune mechanism might influence survival. aim our study characterize cytokine response patients VHF...
Abstract Ticks act as vectors of many pathogens domestic animals and humans. Anaplasma phagocytophilum in Europe is transmitted by the ixodid tick vector Ixodes ricinus . A. causes a disease with diverse clinical signs various hosts. A great genetic diversity groESL operon has been found ticks elsewhere. In Slovenia, variety was conducted only on deer samples. this study, prevalence infected estimated evaluated. On 8 locations 1924 5049 (6973) I. were collected from vegetation years 2005...
West Nile virus (WNV) is a flavivirus transmitted by mosquitoes. Birds are the reservoir for virus; humans, horses and other mammals dead-end hosts. Infections caused WNV in humans can vary from asymptomatic infections to fever (WNF) or neuroinvasive disease (WNND). In 1995, serosurvey was performed Slovenia on forest workers, specific IgG antibodies were confirmed 6.8% of screened samples, indicating that circulating Slovenia. No human cases detected until 2013, when first case infection...
Abstract Purpose Data on the molecular epidemiology of methicillin-resistant Staphylococcus aureus isolates from patients with bacteraemia in Slovenia are lacking. The aim this study was to phenotypically and genotypically investigate 82 MRSA strains isolated bloodstream infections central between 2019 2022. Methods Whole-genome sequencing selected performed characterize based sequence typing, antimicrobial resistance, toxin, virulence factors genes. Results Most carried SCC mec II (63.4%),...
The global spread of antimicrobial resistance genes (ARGs) in
The diarrheagenic Escherichia coli (DEC) is the major cause of diarrheal diseases in Africa, including Ethiopia. However, genetic diversity E. pathotypes found Ethiopia has not been studied well. This study aimed to characterize potential DEC belonging enteropathogenic (EPEC), Shiga toxin-producing (STEC), enteroaggregative (EAEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) from stool specimens patients attending primary healthcare units (n = 260) Addis Ababa Hossana using...
Lyme borreliosis (LB), tick-borne encephalitis (TBE) and human granulocytic anaplasmosis (HGA) are endemic in central part of Slovenia. We tested the hypothesis that patients with erythema migrans (EM) from this region, who have leukopenia and/or thrombocytopenia (typical findings HGA initial phase TBE but not LB) coinfected Anaplasma phagocytophilum virus, i.e. cytopenia is a result concomitant or TBE. Comparison clinical laboratory for 67 EM disclosed leukopenia/thrombocytopenia...
Anaplasma phagocytophilum is an emerging tick-borne pathogen. Great genetic diversity of A. has been described in animals and ticks. The present study focused on the variability groESL operon human patients Slovenia. During 1996–2008, there were 66 serologically confirmed with granulocytic anaplasmosis. Of these, 46 tested a screening PCR for small part 16S rRNA gene 28 (60.9%) positive. Positive samples additionally targeting larger fragment gene. All amplicons further sequenced analyzed....
The density and spread of tick vector species have increased throughout Europe in the last 30 years, leading to an increase Lyme borreliosis cases, including Slovenia.The aim this study was isolate Borrelia strains determine prevalence B. burgdorferi sensu lato miyamotoi adults Ixodes ricinus (Linnaeus) collected 2019 two regions country (Coastal-Karst Littoral-Inner Carniola) by cultivation PCR.We isolated s.l. culture method 28/559 (5%) ticks from both regions.Culture-negative samples...
Bacteria of the genus
The effective identification of bacterial and fungal isolates is essential for microbiological monitoring in environments like speleotherapeutic caves. This study compares MALDI-TOF MS the OmniLog ID System, two high-throughput culture-based methods. identified 80.0% to species level, while System 92.9%. However, species-level matches between methods were only 48.8%, revealing considerable discrepancies. For discrepant results, matched molecular at genus level 90.5% cases, 28.6%,...
Aim We prospectively evaluated four different microbiological tools for diagnostics of prosthetic joint infections (PJI), and assessed their impact on the categorization infection according to EBJIS guidelines. compared culture, in-house real-time mPCR S. aureus, lugdunensis, hominis, epidermidis, capitis, haemolyticus, C. acnes (mPCR), broad-spectrum PCR (Molzym) with 16S rRNA V3-V4 amplicon Sanger sequencing (16S PCR), next-generation NGS) MiSeq (Ilumina). Methods A total 341 samples...
Aim To date, no ultimate diagnostic gold standard for prosthetic joint infections (PJI) has been established. In recent years, next generation sequencing (NGS) emerged as a promising new tool, especially in culture-negative samples. this prospective study, we performed metagenomic analysis using 16S rRNA V3-V4 amplicon NGS samples from patients with suspected PJI. Methods A total of 257 (187 (CN) and 70 culture-positive (CP)) prospectively collected tissues sonication fluid 32 (56 revisions)...
Aim The aim of this study was to develop an in-house multiplex PCR real-time assay on the LightCycler 480 system (Roche, Basel, Switzerland) with rapid detection common pathogens in prosthetic joint infections (PJI), followed by validation clinical samples (sonication fluid and tissue biopsies) routinely collected for PJI diagnosis. Methods Using PrimerQuest CLC WorkBench tool, we designed six primer sets specific fluorescently labelled TaqMan probes nuc gene different Staphylococcus species...
Leptospirosis is an important worldwide zoonosis, and it has also been reported in Slovenia. The cultivation of Leptospira from human material difficult. Despite that, we successfully isolated 12 strains patients between 2002 2020 used various methods for the phenotypic genotypic characterization strains, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) using our own MALDI-TOF data library, melting temperature analysis amplified lfb1 gene,...
Aim The aim of our study was to analyze putative genes for virulence factors Cutibacterium isolates obtained from implant-associated infections. Methods We analyzed 64 spp. ( C. acnes (53/64), avidum (6/64), granulosum (4/64), namnetense (1/64)) using NextSeq 550 (Illumina, San Diego, CA, USA) and performed genomic analysis 24 associated with (VFs) previously reported in the literature. Most were infections (IAI) between 2012–2021 at Institute Microbiology Immunology, Faculty Medicine,...