A5057907254- Mycobacterium research and diagnosis
- Infectious Diseases and Mycology
- Tuberculosis Research and Epidemiology
- Fungal Infections and Studies
- Parasites and Host Interactions
- Helminth infection and control
- Parasite Biology and Host Interactions
- Parasitic infections in humans and animals
- Research on Leishmaniasis Studies
- Infections and bacterial resistance
- Quinazolinone synthesis and applications
- Mollusks and Parasites Studies
- Veterinary medicine and infectious diseases
- Wound Healing and Treatments
- Leptospirosis research and findings
- Infectious Diseases and Tuberculosis
- Congenital Anomalies and Fetal Surgery
- Carbohydrate Chemistry and Synthesis
- Polyamine Metabolism and Applications
- Ginseng Biological Effects and Applications
- Climate Change, Adaptation, Migration
- Dermatological diseases and infestations
- Humic Substances and Bio-Organic Studies
- Cardiac Valve Diseases and Treatments
- Autophagy in Disease and Therapy
Swiss Tropical and Public Health Institute
2016-2025
University of Basel
2015-2025
Instituto Tecnológico de Costa Rica
2023
Universidad de Costa Rica
2023
Inserm
2023
Santé Publique France
2023
Institut Pasteur
2023
Humboldt-Universität zu Berlin
2023
University of Lucerne
2023
Noguchi Memorial Institute for Medical Research
2015
Mycolactone is a diffusible lipid secreted by the human pathogen Mycobacterium ulcerans, which induces formation of open skin lesions referred to as Buruli ulcers. Here, we show that mycolactone operates hijacking Wiskott-Aldrich syndrome protein (WASP) family actin-nucleating factors. By disrupting WASP autoinhibition, leads uncontrolled activation ARP2/3-mediated assembly actin in cytoplasm. In epithelial cells, mycolactone-induced stimulation ARP2/3 concentrated perinuclear region,...
Buruli ulcer (BU), a neglected tropical disease of the skin, caused by Mycobacterium ulcerans, occurs most frequently in children West Africa. Risk factors for BU include proximity to slow flowing water, poor wound care and not wearing protective clothing. Man-made alterations environment have been suggested lead increased incidence. M. ulcerans DNA has detected environment, water bugs recently also mosquitoes. Despite these findings, mode transmission remains poorly understood both insects...
Mycolactone, the macrolide exotoxin produced by Mycobacterium ulcerans, is central to pathogenesis of chronic necrotizing skin disease Buruli ulcer (BU). Here we show that mycolactone acts as an inhibitor mechanistic Target Rapamycin (mTOR) signaling pathway interfering with assembly two distinct mTOR protein complexes mTORC1 and mTORC2, which regulate different cellular processes. Inhibition rictor containing mTORC2 complex prevents phosphorylation serine/threonine kinase Akt. The...
Abstract Mycobacterium ulcerans is the causative agent of Buruli ulcer, a neglected tropical skin disease that most commonly found in children from West and Central Africa. Despite severity infection, therapeutic options are limited to antibiotics with severe side effects. Here, we show M. susceptible anti-tubercular drug Q203 related compounds targeting respiratory cytochrome bc 1 :aa 3 . While primary terminal oxidase tuberculosis , presence an alternate bd-type limits bactericidal...
Background Surveillance of human leishmaniasis in Europe is mostly limited to country-specific information from autochthonous infections the southern part. As at end 2021, no integrated analysis has been performed for cases seen across centres different European countries. Aim To provide a broad perspective on and imported endemic non-endemic countries Europe. Methods We retrospectively collected records cutaneous, mucosal visceral diagnosed 15 between 2014 2019. Centres were located 11...
Genomic studies on pathogenic and environmental mycobacteria are of growing interest for understanding their evolution, distribution, adaptation, host-pathogen interaction. Since most slow growers, material from in vitro cultures is usually scarce. The robust mycobacterial cell wall hinders both experimental lysis efficient DNA extraction. Here, we compare elements several preparation protocols describe a method that economical practical reliably yields large amounts--usually 10-fold...
Buruli ulcer (BU) is an emerging necrotizing disease of the skin and subcutaneous tissue caused by Mycobacterium ulcerans. While proximity to stagnant or slow flowing water bodies a risk factor for acquiring BU, epidemiology mode M. ulcerans transmission poorly understood. Here we have used high-throughput DNA sequencing comparisons genomes seven isolates that appeared monomorphic existing typing methods. We identified limited number single nucleotide polymorphisms (SNPs) developed real-time...
Buruli ulcer (BU), caused by Mycobacterium ulcerans is a chronic necrotizing skin disease. It usually starts with subcutaneous nodule or plaque containing large clusters of extracellular acid-fast bacilli. Surrounding tissue destroyed the cytotoxic macrolide toxin mycolactone produced microcolonies M. ulcerans. Skin covering fat and soft may eventually break down leading to formation ulcers that progress, if untreated, over months years. Here we have analyzed bacterial flora BU lesions three...
Background The neglected tropical disease Buruli ulcer (BU) caused by Mycobacterium ulcerans is an infection of the subcutaneous tissue leading to chronic ulcerative skin lesions. Histopathological features are progressive necrosis, extracellular clusters acid fast bacilli (AFB) and poor inflammatory responses at site infection. After recommended eight weeks standard treatment with rifampicin streptomycin, a reversal local immunosuppression macrolide toxin mycolactone M. observed....
A 66-year-old woman presents with subacute fever, fatigue and weight loss, as well striking eosinophilia hepatic lesions. Diagnostic workup including sequencing of the COX-1 gene from biopsy material ultimately reveals Fasciola hepatica infection, a zoonotic disease rarely seen in Germany. Treatment triclabendazole achieved rapid, full recovery.
Buruli ulcer (BU), a neglected tropical disease of the skin and subcutaneous tissue, is caused by Mycobacterium ulcerans third most common mycobacterial after tuberculosis leprosy. While there strong association occurrence with stagnant or slow flowing water bodies, exact mode transmission BU not clear. M. has emerged from environmental fish pathogen marinum acquisition virulence plasmid encoding enzymes required for production cytotoxic macrolide toxin mycolactone, which key factor in...
A specific and sensitive serodiagnostic test for Mycobacterium ulcerans infection would greatly assist the diagnosis of Buruli ulcer also facilitate seroepidemiological surveys. By comparative genomics, we identified 45 potential M. proteins, which were able to express purify 33 in E. coli. Sera from 30 confirmed patients, 24 healthy controls same endemic region a non-endemic Benin screened antibody responses these proteins by ELISA. Serum IgG patients highly variable, however, seven...
Buruli ulcer (BU) is a chronic necrotizing skin disease caused by Mycobacterium ulcerans, which has been reported worldwide from more than 30 mainly tropical and subtropical countries,1,2 with the ...
Buruli ulcer (BU), caused by infection with Mycobacterium ulcerans, is a chronic necrotizing human skin disease associated the production of cytotoxic macrolide exotoxin mycolactone. Despite extensive research, type immune responses elicited against this pathogen and effector functions conferring protection BU are not yet fully understood. While histopathological analyses advanced lesions have demonstrated mainly extracellular localization toxin producing acid fast bacilli, there growing...
Buruli ulcer (BU) is a neglected tropical disease caused by subcutaneous infection with Mycobacterium ulcerans and its exotoxin mycolactone. BU displays coagulative necrosis widespread fibrin deposition in affected skin tissues. Despite this, the role of vasculature pathogenesis remains almost completely unexplored. We hypothesise that fibrin-driven ischemia can be an 'indirect' route to mycolactone-dependent tissue mechanism involving vascular dysfunction. Here, we tracked >900 vessels...
Buruli ulcer (BU) caused by Mycobacterium ulcerans is a necrotizing skin disease usually starting with subcutaneous nodule or plaque, which may ulcerate and progress, if untreated, over months years. During the currently recommended antibiotic treatment rifampicin/streptomycin plaque lesions tend to ulcerate, often associated retarded wound healing prolonged hospital stays.Included in this study were twelve laboratory reconfirmed, HIV negative BU patients presenting at CDTUB Allada, Benin....
The Mycobacterium ulcerans exotoxin, mycolactone, is responsible for the immunosuppression and tissue necrosis that characterizes Buruli ulcer. Mycolactone inhibits SEC61-dependent co-translational translocation of proteins into endoplasmic reticulum resultant cytosolic translation triggers degradation mislocalized by ubiquitin-proteasome system. Inhibition SEC61 mycolactone also activates multiple EIF2S1/eIF2α kinases in integrated stress response (ISR). Here we show increased canonical...
Leptospirosis is among the most important zoonotic diseases in (sub-)tropical countries. The research objective was to evaluate accuracy of Serion IgM ELISA EST125M against Microscopic Agglutination Test (MAT = imperfect reference test); assess its ability diagnose acute leptospirosis infections and detect previous exposure leptospires an endemic setting. In addition, estimate overall Leptospira spp. seroprevalence Wiwa indigenous population North-East Colombia. We analysed serum samples...
ABSTRACT The highly immunogenic mycobacterial proteins ESAT-6, CFP-10, and HspX represent potential target antigens for the development of subunit vaccines immunodiagnostic tests. Recently, complete genome sequence revealed absence these coding sequences in Mycobacterium ulcerans , causative agent emerging human disease Buruli ulcer. Genome reduction acquisition a cytopathic immunosuppressive macrolide toxin plasmid are regarded as crucial emergence this pathogen from its environmental...
Buruli ulcer (BU) is a necrotizing skin disease most prevalent among West African children. The causative organism, Mycobacterium ulcerans, sensitive to temperatures above 37°C. We investigated the safety and efficacy of local heat application device based on phase change material.In II open label single center noncomparative clinical trial (ISRCTN 72102977) under GCP standards in Cameroon, laboratory confirmed BU patients received up 8 weeks treatment. assessed endpoints 'absence specific...
Buruli ulcer (BU) is a slowly progressing, necrotising disease of the skin caused by infection with Mycobacterium ulcerans. Non-ulcerative manifestations are nodules, plaques and oedema, which may progress to ulceration large parts skin. Histopathologically, BU characterized coagulative necrosis, fat cell ghosts, epidermal hyperplasia, clusters extracellular acid fast bacilli (AFB) in subcutaneous tissue lack major inflammatory infiltration. The mode transmission not clear there only limited...
Background Buruli ulcer is a neglected tropical disease of the skin that caused by infection with Mycobacterium ulcerans. We recently established an experimental pig (Sus scrofa) model for to investigate host-pathogen interactions, efficacy candidate vaccines and new treatment options. Methodology/Principal Findings Here we have used study pathogenesis early interactions in affected porcine upon mycolactone-producing non-producing M. ulcerans strains. Histopathological analyses nodular...
Abstract Background Despite the high prevalence of strongyloidiasis in Laotian population, hospitals still lack diagnostic capacity to appropriately diagnose Strongyloides stercoralis infections. This cross-sectional hospital-based study was conducted assess infection among hospitalized patients treated at Mahosot Hospital, primary reference hospital Lao People’s Democratic Republic (Lao PDR), and validate feasible methods for diagnosing S. hospital’s laboratory. Methods Between September...
A single dose of Q203 (Telacebec), a phase 2 clinical candidate for tuberculosis, eradicates Mycobacterium ulcerans in mouse model Buruli ulcer infection without relapse up to 19 weeks posttreatment. Clinical use may dramatically simplify the management ulcer, neglected mycobacterial disease.