A5019634944- T-cell and B-cell Immunology
- Mycobacterium research and diagnosis
- Immune Cell Function and Interaction
- Reproductive tract infections research
- Hematopoietic Stem Cell Transplantation
- Infectious Diseases and Mycology
- Herpesvirus Infections and Treatments
- Ethics in Clinical Research
- Urinary Tract Infections Management
- Immunotherapy and Immune Responses
- Health Systems, Economic Evaluations, Quality of Life
- Tuberculosis Research and Epidemiology
- Genetic Neurodegenerative Diseases
- CAR-T cell therapy research
- Adolescent Sexual and Reproductive Health
- Botulinum Toxin and Related Neurological Disorders
- Fungal Infections and Studies
- CRISPR and Genetic Engineering
- Neurological disorders and treatments
- Aquaculture disease management and microbiota
- Health Policy Implementation Science
- Poxvirus research and outbreaks
- Plant and fungal interactions
- Veterinary medicine and infectious diseases
- Toxin Mechanisms and Immunotoxins
Wellcome Sanger Institute
2016-2022
Multiple Sclerosis Society of Canada
2020-2022
Universitat Autònoma de Barcelona
2021
Institute of Science and Technology
2020
Centre for Genomic Regulation
2020
Swiss Tropical and Public Health Institute
2010-2016
University of Basel
2006-2016
Albert Einstein College of Medicine
2015
Benaroya Research Institute
2003
Virginia Mason Medical Center
2003
CD4+CD25+ regulatory T (TR) cells have been described in both humans and mice. In mice, TR are thymically derived, lack of leads to organ-specific autoimmunity. Recently, the forkhead/winged helix transcription factor, FoxP3, has shown be important for function this study, human were examined and, results similar those studies done expression FoxP3 was found exclusively correlated with suppressive activity these cells. contrast mouse studies, activation CD4+CD25– led FoxP3. Expression...
Subunit vaccines comprised of glycoprotein D (gD-2) failed to prevent HSV-2 highlighting need for novel strategies. To test the hypothesis that deletion gD-2 unmasks protective antigens, we evaluated efficacy and safety an virus deleted in complemented allowing a single round replication on cells expressing HSV-1 gD (ΔgD−/+gD−1). Subcutaneous immunization C57BL/6 or BALB/c mice with ΔgD−/+gD1 provided 100% protection against lethal intravaginal skin challenges prevented latency. elicited no...
CD4+CD25+ regulatory T (TR) cells have been described in both humans and mice. In mice, TR are thymically derived, lack of leads to organ-specific autoimmunity. Recently, the forkhead/winged helix transcription factor, FoxP3, has shown be important for function this study, human were examined and, results similar those studies done expression FoxP3 was found exclusively correlated with suppressive activity these cells. contrast mouse studies, activation CD4+CD25– led FoxP3. Expression...
Lymphogranuloma venereum (LGV), the invasive infection of sexually transmissible (STI) Chlamydia trachomatis , is caused by strains from LGV biovar, most commonly represented ompA -genotypes L2b and L2. We investigated diversity in samples across an international collection over seven years using typing genome sequencing. LGV-positive ( n =321) eight countries collected between 2011 2017 (Spain =97, Netherlands =67, Switzerland =64, Australia =53, Sweden =37, Hungary =31, Czechia =30,...
A specific and sensitive serodiagnostic test for Mycobacterium ulcerans infection would greatly assist the diagnosis of Buruli ulcer also facilitate seroepidemiological surveys. By comparative genomics, we identified 45 potential M. proteins, which were able to express purify 33 in E. coli. Sera from 30 confirmed patients, 24 healthy controls same endemic region a non-endemic Benin screened antibody responses these proteins by ELISA. Serum IgG patients highly variable, however, seven...
Mycobacterium tuberculosis (Mtb) possesses five type VII secretion systems (T7SS), virulence determinants that include the apparatus and associated substrates. Mtb strains deleted for genes encoding substrates of ESX-3 T7SS, esxG or esxH, require iron supplementation in vitro growth are highly attenuated vivo. In a subset infected mice, suppressor mutants esxH deletions were isolated, which enabled to high titers restored virulence. Suppression was conferred by mechanisms cause...
Background Buruli ulcer, caused by infection with Mycobacterium ulcerans, is a chronic ulcerative neglected tropical disease of the skin and subcutaneous tissue that most prevalent in West African countries. M. ulcerans produces cytotoxic macrolide exotoxin called mycolactone, which causes extensive necrosis infected development characteristic lesions undermined edges. While cellular immune responses are expected to play key role against early intracellular stages macrophages, antibody...
Sublethally irradiated, immunodeficient, C57BL/6 RAG-2 gene-deleted recipient mice reconstituted with T cell-depleted bone marrow (BM) grafts frequently developed diarrhea, lost weight and showed signs of autoimmunity, dying between 4 7 weeks after reconstitution. Mice died despite evidence efficient donor-derived hemato-lymphoid reconstitution, disease was associated the presence IgG anti-nuclear antibodies. Autoimmunity initiated by cells, but could be prevented transfer naturally arising...
Buruli Ulcer is a neglected tropical disease leading to extensive disabilities and morbidity in West Africa. In this paper we sought characterize various strains of Mycobacterium ulcerans (M.ulcerans) with different origins laboratory passage records while refining mouse model for ulcer. We described, compared followed the kinetics histo-pathological outcome infection collection at anatomical sites order find suitable further immunization studies. Moreover C57Bl/6 Balbc/J mice. Specifically...
<ns4:p><ns4:bold>Background:</ns4:bold>Research in chlamydial genetics is challenging because of its obligate intracellular developmental cycle.<ns4:italic>In vivo</ns4:italic>systems exist that allow studies different aspects basic biology chlamydiae, the murine<ns4:italic>Chlamydia muridarum</ns4:italic>model one great importance and thus an essential research tool.<ns4:italic>C. muridarum</ns4:italic>carries a plasmid has role virulence. Our aim was to compare contrast the<ns4:italic>C....
Global estimates for cholera annually approximate 4 million cases worldwide with 95,000 deaths. Recent outbreaks, including Haiti and Yemen, are reminders that is still a global health concern. Cholera outbreaks can rapidly induce high death tolls by overwhelming the capacity of facilities, especially in remote areas or civil unrest. studies demonstrated stool specimens preserved on filter paper facilitate molecular analysis Vibrio cholerae resource limited settings. Specimens rapid,...
Between 10 and 20% of the peripheral gammadelta T cells express cytoplasmic TCR-beta proteins, but whether such chains can partake in alphabeta T-cell development has never been systematically investigated. Therefore, we reconstituted compartment CD3epsilon-deficient mice with Pax5-TCR-beta deficient proB expressing, via a retroviral vector, from either or cells. Recipient thymi containing empty vector were small (<15x10(6) cells), contained few no In contrast, receiving T-cell-derived...
Objectives Disease-modifying therapies in development for Huntington’s disease (HD) may require specialised administration and additional resource capacity. We sought to understand current future capacity HD management Canada considering the possible introduction of an intrathecal (IT) disease-modifying treatment (DMT). Design, setting participants Using a case study, mixed methods framework, online surveys followed by semistructured interviews were conducted late 2020 early 2021....
Under-recruitment regularly impedes clinical trials, leading to wasted resources and opportunity costs. Methods for designing trial participation strategies rarely consider behavior change theory.Informed by the Theoretical Domains Framework, we identified factors important participating in Huntington's disease research provide examples of how such a theory-informed approach can make specific suggestions about design targeted recruitment strategies.We range barriers enablers based on...
The COVID-19 pandemic has posed and is continuously posing enormous societal health challenges worldwide. research community mobilized to develop novel projects find a cure or vaccine, as well contribute mass testing, which been critical measure contain the infection in several countries. Through this article, we share our experiences learnings group of volunteers at Centre for Genomic Regulation (CRG) Barcelona, Spain. As members ORFEU project, an initiative by Government Catalonia achieve...