A5056325210- Leprosy Research and Treatment
- Mycobacterium research and diagnosis
- Research on Leishmaniasis Studies
- Infectious Diseases and Tuberculosis
- Trypanosoma species research and implications
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Ocular Diseases and Behçet’s Syndrome
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Urticaria and Related Conditions
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Parasites and Host Interactions
- vaccines and immunoinformatics approaches
- Insect and Pesticide Research
- Psoriasis: Treatment and Pathogenesis
- Hepatitis B Virus Studies
- Yersinia bacterium, plague, ectoparasites research
- Hepatitis Viruses Studies and Epidemiology
- Cytomegalovirus and herpesvirus research
TRIA Bioscience (United States)
2020-2022
Infectious Disease Research Institute
2012-2021
Seattle University
2011-2020
Io Therapeutics (United States)
2020
490 BioTech (United States)
2020
Aptevo Therapeutics (United states)
2019
University of Washington
2016-2019
National Council for Scientific and Technological Development
2018
Adjuvance (United States)
2018
GTx (United States)
2018
Innate immune responses to vaccine adjuvants based on lipopolysaccharide (LPS), a component of Gram-negative bacterial cell walls, are driven by Toll-like receptor (TLR) 4 and adaptor proteins including MyD88 TRIF, leading the production inflammatory cytokines, type I interferons, chemokines. We report here characterization synthetic hexaacylated lipid A derivative, denoted as glucopyranosyl adjuvant (GLA). assessed effects GLA murine human dendritic cells (DC) combining microarray, mRNA...
The coronavirus disease 2019 (COVID-19) pandemic, caused by infection with the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is having a deleterious impact on health services and global economy, highlighting urgent need for an effective vaccine. Such vaccine would to rapidly confer protection after one or two doses be manufactured using components suitable scale up. Here, we developed
ABSTRACT Leprosy is a chronic and debilitating human disease caused by infection with the Mycobacterium leprae bacillus. Despite marked reduction in number of registered worldwide leprosy cases as result widespread use multidrug therapy, new detected each year remains relatively stable. This indicates that M. still being transmitted that, without earlier diagnosis, will continue to pose health problem. Current diagnostic techniques, based on appearance clinical symptoms or immunoglobulin M...
Key antigens of Leishmania species identified in the context host responses ‐exposed individuals from disease‐endemic areas were prioritized for development a subunit vaccine against visceral leishmaniasis (VL), most deadly form leishmaniasis. Two proteins—nucleoside hydrolase and sterol 24‐c‐methyltransferase, each which are protective animal models VL when properly adjuvanted— produced as single recombinant fusion protein NS (LEISH‐F3) ease antigen production broad coverage heterogeneous...
Safe, effective adjuvants that enhance vaccine potency, including induction of neutralizing Abs against a broad range variant strains, is an important strategy for the development seasonal influenza vaccines which can provide optimal protection, even during seasons when available are not well matched to circulating viruses. We investigated safety and ability Glucopyranosyl Lipid Adjuvant-Stable Emulsion (GLA-SE), synthetic Toll-like receptor (TLR)4 agonist formulation, adjuvant Fluzone® in...
Nine-banded armadillos (Dasypus novemcinctus) are naturally infected with Mycobacterium leprae and have been implicated in zoonotic transmission of leprosy. Early studies found this disease mainly Texas Louisiana, but the southeastern United States appeared to be free infection. We screened 645 from 8 locations not known harbor enzootic leprosy for M. DNA antibodies. leprae-infected at each location, 106 (16.4%) animals had serologic/PCR evidence Using single-nucleotide polymorphism variable...
Interleukin 17 (IL-17) is an inflammatory cytokine that plays a protective role against intracellular parasites. The of IL-17 during Leishmania infection remains controversial and poorly defined. We evaluated whether participates in the host immune response to infantum. IL-17A present sera from patients with visceral leishmaniasis decreases after successful treatment. In C57BL/6 infected mice, higher production coincided peak parasitism. Il17ra−/− mice were more susceptible also exhibited...
Background Visceral leishmaniasis (VL) is a severe disease caused by infection with protozoa of the genus Leishmania. Classic VL characterized systemic phagocytic cells and an intense activation inflammatory response. It unclear why 90% infected individuals do not develop while minority classical form. Furthermore, among those that disease, small group progresses to more form unresponsive treatment. The presence mediators in serum could theoretically help control infection. However, there...
RNA vaccines possess significant clinical promise in counteracting human diseases caused by infectious or cancerous threats. Self-amplifying replicon (repRNA) has been thought to offer the potential for enhanced potency and dose sparing. However, repRNA is a potent trigger of innate immune responses vivo, which can cause reduced transgene expression dose-limiting reactogenicity, as highlighted recent trials. Here, we report that multivalent vaccination, necessitating higher doses total RNA,...
Abstract Background Leprosy is a chronic infectious disease caused by Mycobacterium leprae that can manifest wide variety of immunological and clinical outcomes ranging from potent humoral responses among borderline lepromatous (BL) (LL) patients to strong cellular tuberculoid (TT) (BT) patients. Until recently, relatively little has been known about the immune individual proteins M. recognized during leprosy. Methods The reactivity panel 33 recombinant was evaluated leprosy controls high...
Leprosy remains an important health problem in a number of regions. Early detection infection, followed by effective treatment, is critical to reduce disease progression. New sensitive and specific tools for early infection will be component leprosy elimination campaign. Diagnosis made recognizing clinical signs symptoms, but few clinicians are able confidently identify these. Simple tests facilitate referral experts not widely available, the correct diagnosis often delayed. In this report,...
Abstract Therapy of intracellular pathogens can be complicated by drug toxicity, resistance, and the need for prolonged treatment regimens. One approach that has shown promise is immunotherapy. Leishmaniasis, a vector-borne disease ranked among six most important tropical infectious diseases World Health Organization, been treated clinically with crude or defined vaccine preparations cytokines, such as IFN-γ GM-CSF, in combination chemotherapy. We have attempted to develop an improved...
Abstract Background Despite efforts to eliminate leprosy as public health problem, delayed diagnosis and disabilities still occur in many countries. Leprosy remains based on clinical manifestations the number of clinicians with expertise is decline. We have developed a new immunochromatographic test goal producing simple rapid system that can be used, minimal amount training, provide an objective consistent multibacillary leprosy. Methods The immobilizes two antigens been recognized...
Sustained elimination of Visceral Leishmaniasis (VL) requires the reduction and control parasite reservoirs to minimize transmission Leishmania donovani infection. A simple, reproducible definitive diagnostic procedure is therefore indispensable for early accurate detection parasites in VL, Relapsed VL (RVL) Post Kala-azar Dermal (PKDL) patients, all whom are potential parasites. To overcome limitations current approaches, a novel quantitative real-time polymerase chain reaction (qPCR)...
Background Leprosy diagnosis is mainly based on clinical evaluation, although this approach difficult, especially for untrained physicians. We conducted a temporary campaign to detect previously unknown leprosy cases in midwestern Brazil and compare the performance of different serological tests. Methods A mobile clinic was stationed at main bus terminal Brasília, Brazil. Volunteers were quizzed given exam allow categorization as either patients, known contacts patients or non-contacts,...
Two million new cases of leishmaniasis occur every year, with the cutaneous (CL) presentation accounting for approximately two-thirds all cases. Despite high incidence rates and geographic expansion disease, CL remains a neglected tropical disease without effective intervention strategies. Efforts to address this deficit have given rise experimental murine model CL. By virtue its simplicity pliability, has been used provide substantial information regarding cellular immunity, as well in...
Recent reports that TLR4 and TLR7 ligands can synergistically trigger Th1 biased immune responses suggest an adjuvant contains both would be excellent candidate for co-administration with vaccine antigens which heavily are desired. Ligands of each these TLRs generally have disparate biochemical properties, however, straightforward co-formulation may represent obstacle. We show here the ligand, imiquimod, GLA, in human whole blood. combined anionic liposomal formulation where ligand is...
The synthetic TLR4 agonist glucopyranosyl lipid adjuvant (GLA) is a potent Th1‐response‐inducing when formulated in squalene oil‐in‐water emulsion (SE). While the innate signals triggered by engagement are well studied, contribution of SE remains unclear. To better understand effect on properties GLA‐SE, we compared and adaptive immune responses elicited immunization with different formulations: GLA without oil, alone or combination, mice. Within response to adjuvants, only GLA‐SE displayed...
Serological tests can be important tools to assist in the diagnosis of leprosy and contribute an earlier diagnosis. The aim this study was evaluate antibody responses against phenolic glycolipid-1 (PGL-1), natural disaccharide linked human serum albumin via octyl (NDO-HSA), Leprosy IDRI Diagnostic-1 (LID-1) - (NDO-LID) patients, household contacts patients general population. Enzyme-linked immunosorbent assays were used analyze antigen-specific responsesof 94 cases, 104 cases 2.494...