A5037849868- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Blood disorders and treatments
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Acute Lymphoblastic Leukemia research
- Cancer Immunotherapy and Biomarkers
- Childhood Cancer Survivors' Quality of Life
- Epigenetics and DNA Methylation
- Polyomavirus and related diseases
- Virus-based gene therapy research
- Transplantation: Methods and Outcomes
- Pharmacological Effects of Natural Compounds
- Mesenchymal stem cell research
- Blood groups and transfusion
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- CRISPR and Genetic Engineering
- Parvovirus B19 Infection Studies
- Glycosylation and Glycoproteins Research
- Renal Transplantation Outcomes and Treatments
- Cell Adhesion Molecules Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Central Venous Catheters and Hemodialysis
Nagoya University
2014-2023
Cleveland Clinic
2022-2023
Cleveland Clinic Lerner College of Medicine
2023
Japanese Red Cross Nagoya Daiichi Hospital
2012-2019
Suzuki (Japan)
2016
Yokohama City University
2015
Hokkaido University Hospital
2008-2011
New York University
2003-2009
Koen Gakuen Women's Junior College
2008
Tokyo Medical and Dental University
2006
Purpose Acute lymphoblastic leukemia (ALL) makes up a significant proportion of all pediatric cancers, and relapsed ALL is leading cause cancer-associated deaths in children. Identification risk factors druggable molecular targets can lead to better stratification treatments subsequent improvement prognosis. Patients Methods We enrolled 59 children with or primary refractory who were treated our institutions. primarily performed RNA sequencing (RNA-seq) using patients’ leukemic cells...
Acquired aplastic anemia is an immune-mediated disease characterized by severe defects in stem cell number resulting hypocellular marrow and peripheral blood cytopenias. Minor paroxysmal nocturnal hemoglobinuria populations a short telomere length were identified as predictive biomarkers of immunosuppressive therapy responsiveness anemia. We enrolled 113 patients (63 boys 50 girls) this study to evaluate their response therapy. The detected flow cytometry. Forty-seven (42%) carried minor...
Shwachman-Diamond syndrome is a rare inherited bone marrow failure characterized by neutropenia, exocrine pancreatic insufficiency, and skeletal abnormalities. In 10-30% of cases, transformation to myeloid neoplasm occurs. Approximately 90% patients have biallelic pathogenic variants in the SBDS gene located on human chromosome 7q11. Over past several years, three other genes been identified cause similar phenotypes; these are DNAJC21, EFL1, SRP54. Clinical manifestations involve multiple...
Adoptive T cell therapy using chimeric antigen receptor (CAR)-modified cells is a promising cancer immunotherapy. We previously developed non-viral method of gene transfer into piggyBac transposon system to improve the cost-effectiveness CAR-T therapy. Here, we have further improved our technology by novel culture strategy increase transfection efficiency and reduce time manufacturing. Using CH2CH3-free CD19-specific CAR vector combining irradiated activated (ATCs) as feeder virus-specific...
Predicting the response to immunosuppressive therapy could provide useful information help clinician define treatment strategies for patients with aplastic anemia. In our current study, we evaluated relationship between telomere length of lymphocytes at diagnosis and in 64 children anemia, using flow fluorescence situ hybridization. Median age was ten years (range 1.5-16.2 years). Severity disease classified as very severe 23, 21, moderate 20 patients. All were enrolled multicenter studies...
Insertional mutagenesis is an important risk with all genetically modified cell therapies, including chimeric antigen receptor (CAR)-T therapy used for hematological malignancies. Here we describe a new tagmentation-assisted PCR (tag-PCR) system that can determine the integration sites of transgenes without using restriction enzyme digestion (which potentially bias detection) and allows library preparation in fewer steps than other methods. Using this system, compared CD19-specific CAR genes...
We assessed the clinical utility of next-generation sequencing (NGS)-based monitoring minimal residual disease (MRD) in a uniformly treated cohort 79 patients with paediatric B-cell acute lymphoblastic leukaemia. Bone marrow samples were collected at time diagnosis, days 33 and 80, pre- (4-5 months) post- (24 maintenance therapy points, relapse. identified leukaemia-specific CDR3 sequences 72 (91%) detected MRD 59 232 samples. Although was 28 55 (51%) on day 33, frequencies detection...
Therapy for BK virus (BKV)-associated hemorrhagic cystitis (BKV-HC) is limited after hematopoietic stem cell transplantation (HSCT). We examined whether choreito, a formula from Japanese traditional Kampo medicine, effective treating BKV-HC. Among children who underwent allogeneic HSCT between October 2006 and March 2014, 14 were diagnosed with BKV-HC (median, 36 days; range, to 330 days) HSCT, 6 consecutive received pharmaceutical-grade choreito extract granules. The hematuria grade before...
Fibrolamellar carcinoma (FLC), a variant of hepatocellular (HCC), very rarely occurs in association with cholangiocarcinoma (CC). This report describes the first case FLC coexisting CC (FLC-CC) from Japan. Although major part tumour located right lobe liver showed typical features FLC, was admixed not only primary hepatic tumour, but also lymph node metastases. Immunohistochemical analysis revealed that, although carcinoembryonic antigen (CEA), which can be detected monoclonal antibodies...
Summary The clinical significance of paroxysmal nocturnal haemoglobinuria ( PNH ) in children with aplastic anaemia AA remains unclear. We retrospectively studied 57 between 1992 and 2010. During the follow‐up, five patients developed , whom somatic PIGA mutations were detected by targeted sequencing. 10‐year probability development was 10·2% (95% confidence interval, 3·6–20·7%). Furthermore, detection minor clones flow cytometry at diagnosis a risk factor for subsequent . These should...
The prognosis of adrenoleukodystrophy (ALD)with neurological involvement is generally dismal; however, allogeneic stem cell transplantation (SCT) recognized as effective to stabilize or improve the clinical symptoms ALD. Herein, we report outcomes patients with ALD who consecutively underwent reduced intensity conditioning at our institution.Sixteen ALD, were symptomatic (n = 14) presymptomatic 2), received SCT from 2010 2016. source was cord blood 14), bone marrow a human leukocyte antigen...
PSD‐95/SAP90, which binds to the C‐terminus of NMDA receptor and Shaker‐type potassium channel, is one major postsynaptic density proteins. Recently, novel classes proteins interacting with guanylate kinase domain PSD‐95 have been identified, kinase‐associated protein (GKAP) SAP90/PSD‐95‐associated (SAPAPs). Here we report isolation new isoforms binding (GKAP/SAPAP1) using yeast two‐hybrid system. The isolated directly interacts PSD‐95. Northern blot analyses revealed that expression these...