A5011354096- Erythropoietin and Anemia Treatment
- RNA Research and Splicing
- RNA modifications and cancer
- Erythrocyte Function and Pathophysiology
- RNA and protein synthesis mechanisms
- 3D Printing in Biomedical Research
- Nuclear Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- Iron Metabolism and Disorders
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Pluripotent Stem Cells Research
- Heavy Metal Exposure and Toxicity
- Chromosomal and Genetic Variations
- Hemoglobinopathies and Related Disorders
- DNA Repair Mechanisms
- Virus-based gene therapy research
- PARP inhibition in cancer therapy
- Molecular Biology Techniques and Applications
- Apelin-related biomedical research
- Pharmacological Effects and Toxicity Studies
- Medicinal Plants and Bioactive Compounds
- Blood properties and coagulation
- Angiogenesis and VEGF in Cancer
- RNA regulation and disease
Kindai University
2022-2025
Kyoto University
2012-2024
Hokkaido University
2009-2022
Bio-Medical Science (South Korea)
2017
MRC Laboratory of Molecular Biology
2015
Tokyo Institute of Technology
2011
National Astronomical Observatory of Japan
2011
Tokai University
2011
The Graduate University for Advanced Studies, SOKENDAI
2011
Kyungpook National University
2011
Erythropoietin (EPO) produced by the kidney and liver (in fetuses) stimulates erythropoiesis. In central nervous system, neurons express EPO receptor (EPOR) astrocytes produce EPO. has been shown to protect primary cultured from N -methyl- d -aspartate (NMDA) receptor-mediated glutamate toxicity. Here we report in vivo evidence that protects against ischemia-induced cell death. Infusion of into lateral ventricles gerbils prevented learning disability rescued hippocampal CA1 lethal ischemic...
amines (19). Epo augments choline acetyltransferase in cul-
Although vascular endothelial growth factor (VEGF) is a primary mediator of retinal angiogenesis, VEGF inhibition alone insufficient to prevent neovascularization. Hence, it postulated that there are other potent ischemia-induced angiogenic factors. Erythropoietin possesses activity, but its potential role in ocular angiogenesis not established.We measured both erythropoietin and levels the vitreous fluid 144 patients with use radioimmunoassay enzyme-linked immunosorbent assay. Vitreous...
In yeast, the TREX complex contains THO transcription elongation complex, which functions in direct cotranscriptional recruitment of mRNA export proteins Sub2 and Yra1 to nascent transcripts. Here we report identification human show that it associates with spliced mRNA, but not unspliced pre-mRNA vitro. Transcription is required for this recruitment. We also colocalizes splicing factors nuclear speckle domains vivo. Considering occurs cotranscriptionally humans, our data indicate directly...
Radioiodinated erythropoietin (Epo) was bound specifically to the cells of two non-erythroid clonal lines, PC12 and SN6, which expressed neuronal characteristics. The binding time-, cell number-, dose-dependent reversible. Although cloned Epo receptor from (derived rat adrenal medulla) identical that erythroid cells, significant differences in ligand properties between lineages were found; 1) had a single class sites with very low affinity (Kd = 16 nM), whereas classes different affinities...
Although erythropoietin (Epo) has been shown to possess <i>in vitro</i> angiogenic activity, its physiological significance not demonstrated. Normally angiogenesis does occur actively in adults but an exception is the female reproductive organ. In uterine endometrium, takes place for supporting endometrial growth that occurs during transition from diestrus estrous stage. This under control of 17β-estradiol (E<sub>2</sub>), ovarian hormone, and can be mimicked by injection E<sub>2</sub>...
1. Recombinant human erythropoietin has N-linked sugar [Tsuda et al., (1988) Biochemistry 27, 5646-5654]. Here we have demonstrated the presence of O-linked (0.85 mol/mol erythropoietin) composed sialic acid and Gal beta(1-3)GalNAc. 2. To investigate role these sugars, erythropoietins deglycosylated to different extents were prepared using specific glycosidases. Sugars are not essential for in vitro biological activity erythropoietin, because fully had full when assayed with bioassay...
Abstract Transposable elements (TEs), or transposons, play an important role in adaptation. TE insertion can affect host gene function and provides a mechanism for rapid increases genetic diversity, particularly because many TEs respond to environmental stress. In the current study, we show that transposition of heat-activated retrotransposon, ONSEN , generated mutation abscisic acid (ABA) responsive gene, resulting ABA-insensitive phenotype Arabidopsis suggesting stress tolerance. Our...
Abstract mRNA export is an essential pathway for the regulation of gene expression. In humans, closely related RNA helicases, UAP56 and URH49, shape selective pathways through formation distinct complexes, known as apo-TREX apo-AREX their subsequent remodeling into similar ATP-bound complexes. Therefore, defining unidentified components complex elucidating molecular mechanisms underlying apo-complexes key to understanding functional divergence. this study, we identify additional physically...
Erythropoietin (Epo) produced by the kidney regulates erythropoiesis. Recent evidence suggests that Epo in cerebrum prevents neuron death and uterus induces estrogen (E 2 )-dependent uterine angiogenesis. To elucidate how expression is regulated these tissues, ovariectomized mice were given E and/or exposed to hypoxia, temporal patterns of mRNA levels examined. elevated markedly within 4 h after exposure hypoxia. Although level decreased 8 despite continuous high was sustained for ≥24 h,...
Erythropoietin (Epo) has three N-linked sugar chains. Codons for asparagine at N-glycosylation sites in genomic human Epo DNA were replaced with those glutamine. The wild-type gene and seven mutants that lacked every possible combination introduced into baby hamster-kidney cells. To study the role of sugars biosynthesis, protein expressed transiently was measured by an enzyme-linked immunoassay. elimination all decreased production to 10% Epo. Wild-type mutant Epos produced stably...
The majority of CDF/ZnT zinc transporters form homo-oligomers. However, ZnT5, ZnT6, and their orthologues hetero-oligomers in the early secretory pathway where they load onto zinc-requiring enzymes maintain functions. details this hetero-oligomerization remain to be elucidated, much more is known about homo-oligomerization that occurs other family proteins. Here, we addressed issue using co-immunoprecipitation experiments, mutagenesis, chimera studies hZnT5 hZnT6 chicken DT40 cells deficient...
Nuclear export of mRNA is an essential process for eukaryotic gene expression. The TREX complex couples expression from transcription and splicing to export. Sub2, a core component the in yeast, has diversified humans two closely related RNA helicases, UAP56 URH49. Here, we show that URH49 forms novel URH49-CIP29 complex, termed AREX (alternative export) whereas human complex. mRNAs regulated by these helicases are different at genome-wide level. sets target contain distinct subsets key...
The SLC39A family of zinc transporters can be divided into four subfamilies (I, II, LIV-1, and gufA) in vertebrates, but studies their functions have been restricted exclusively to members II LIV-1. In this study, we characterized SLC39A9 (ZIP9), the only member subfamily I vertebrates. Confocal microscopy demonstrated that transiently expressed, HA-tagged human ZIP9 (hZIP9-HA) was localized trans-Golgi network regardless status. Disruption gene DT40 cells did not change growth rate,...
The accumulating evidence that erythropoietin and receptor are expressed in various non-haematopoietic organs suggests signalling might be involved the growth of tumours, but this possibility has never been examined. We found mRNAs for malignant tumours female reproductive organs, where levels higher than normal tissues. Furthermore, tumour cells capillary endothelium showed immunoreactivity. To investigate role erythropoietin/erythropoietin pathway these we injected mouse monoclonal...
We have recently shown that malignant tumours from the ovary and uterus expressed erythropoietin (Epo) its receptor (EpoR), deprivation of Epo signal in tumour blocks induced death cells capillary endothelial vitro (Yasuda et al, submitted). These results prompted us to examine effect Epo-signal withdrawal on vivo. RT-PCR analysis demonstrated expression mRNAs for EpoR transplants uterine ovarian nude mice. Then we injected locally anti-Epo antibody or soluble form into transplants. At 12 h,...
A number of enzymes become functional by binding to zinc during their journey through the early secretory pathway. The transporters (ZnTs) located there play important roles in this step. We have previously shown that two transport complexes, ZnT5/ZnT6 heterodimers and ZnT7 homo-oligomers, are required for activation alkaline phosphatases, converting them from apo- holo-form. Here, we investigated molecular mechanisms activation. ZnT1 ZnT4 expressed chicken DT40 cells did not contribute...
Transposable elements (TEs) are key that facilitate genome evolution of the host organism. A number studies have assessed functions TEs, which change gene expression in genome. Activation TEs is controlled by epigenetic modifications such as DNA methylation and histone modifications. Several recent reported can also be activated biotic or abiotic stress some plants. We focused on a Ty1/copia retrotransposon, ONSEN, heat Arabidopsis. found transcriptional activation ONSEN was regulated an...
There is growing evidence that eicosapentaenoic acid (EPA) uptake has beneficial effects on various cardiovascular diseases. However, electrophysiological actions of EPA remain poorly documented. To investigate the potential antiarrhythmic EPA, mice were fed a high-fat diet (HFD) or an HFD supplemented with for eight weeks. Electrocardiogram (ECG) recordings in combined esophageal electrical stimulation revealed HFD-fed exhibited bradycardia, reduced P-wave amplitude, and prolonged duration....
Previously, we showed that erythropoietin (Epo) is produced in the mouse uterus, where Epo indispensable for estrogen (E 2 )-dependent angiogenesis. Expression of uterine mRNA stimulated by E and hypoxia. The hypoxic induction requires presence . In present study, examined other female reproductive organs with respect to expression its stimuli hypoxia)-induced changes. Although was seen ovary oviduct, -induced stimulation found only oviduct. oviduct transient rapidly downregulated. hypoxia...