E4BP4, a basic leucine zipper transcription factor, contains DNA-binding domain closely related to DBP, HLF, and TEF, which are PAR proteins. Here, we show that the phase of e4bp4 mRNA rhythm is opposite dbp, hlf , tef rhythms in suprachiasmatic nucleus (SCN), mammalian circadian center, liver. The protein levels E4BP4 DBP also fluctuate almost phase. Moreover, all proteins activate, whereas suppresses, transcriptional activity reporter gene containing common binding sequence assays vitro....

The accumulating evidence that erythropoietin and receptor are expressed in various non-haematopoietic organs suggests signalling might be involved the growth of tumours, but this possibility has never been examined. We found mRNAs for malignant tumours female reproductive organs, where levels higher than normal tissues. Furthermore, tumour cells capillary endothelium showed immunoreactivity. To investigate role erythropoietin/erythropoietin pathway these we injected mouse monoclonal...

We have recently shown that malignant tumours from the ovary and uterus expressed erythropoietin (Epo) its receptor (EpoR), deprivation of Epo signal in tumour blocks induced death cells capillary endothelial vitro (Yasuda et al, submitted). These results prompted us to examine effect Epo-signal withdrawal on vivo. RT-PCR analysis demonstrated expression mRNAs for EpoR transplants uterine ovarian nude mice. Then we injected locally anti-Epo antibody or soluble form into transplants. At 12 h,...

Abstract The administration of a single dose all‐trans retinoic acid on day 8 gestation to pregnant mice, ICR strain, led malformed fetuses in all the litters. All‐trans (RA) was dissolved olive oil and given doses 60 or 40 mg/kg body weight. control mice were vehicle alone. Examination 18 exposed showed various malformations, such as exencephaly, exophthalmus, micrognathia, agnathia, cleft palate, lower lip, spina bifida, atresia ani, tail anomalies, agenesis kidneys, hydronephrosis. In...

The liver is the central organ of metabolism, but its function varies during development from fetus to adult. In this study, we comprehensively analyzed and compared metabolites in fetal adult hepatocytes, major parenchymal cell liver, human donors. We identified 211 (116 anions 95 cations) by capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) hepatocytes cultured vitro. Principal component analysis hierarchical clustering relative amounts clearly classified into 2 groups...

The tissue distribution and function of hemoglobin or myoglobin are well known; however, a newly found cytoglobin (CYGB), which also belongs to the globin family, remains be characterized. To assess its expression in human malignancies, we sought screen number cell lines originated from many tissues using northern blotting real time PCR techniques. Unexpectedly, that several, but not all, melanoma expressed CYGB mRNA protein at much higher levels than cells other origins. Melanocytes,...

Abstract All‐ trans ‐retinoic acid induced 2 types of disorganized neuroepithelium, localized and continuous, in the exencephaly 9‐day‐old mouse embryos exposed to 60 or 40 mg/kg for 27 30 hr utero. The effect appeared as a protuberance wall telencephalon thick neural folds mesencephalon with discontinuity apical terminal sheet. continuous disorganization was seen from olfactory placode myelencephalon rosettes cells many dense bodies neuroepithelium. Ultrastructurally, disorganizations...

Erythropoiesis begins first in the visceral yolk sac (VYS) of embryo; however, involvement erythropoietin (EPO) yolk-sac erythropoiesis has not been studied adequately. This study reports expression EPO normal and hypoxic VYSs alterations components induced by retinoic acid (RA) mice. Gravid mice (plug day = 0 gestation) were given one oral dose 60 mg/kg RA olive oil on days 6, 6.5, 7, 7.5, or 8 gestation sacrificed 2.5, 3, 3.5 later. Control received without RA. None dams developed anemia,...

We analyzed the role of maternal C677T mutation in methylenetetrahydrofolate reductase (MTHFR) gene on spina bifida development newborns. A total 115 mothers who had given birth to a child (SB mothers) gave 10 mL blood together with written informed consent. The genotype distribution was assessed and compared that 4517 control individuals. prevalence homozygous (TT) among SB not significantly different from controls (odds ratio [OR] = 0.65; 95% confidence interval [CI] 0.31-1.25; P 0.182),...

We implemented a Mathematica module of CGA which includes functions to denote elements and compute several operations in CGA. can draw the figure 3D space is corresponding element. Our function using Gr\"{o}bner Basis for simplifying equations figures. It be used any dimensional One our motivations realize origami system own Library. follow 2D computational E-Origami-System developed by Ida et.al. formulated simple fold points motions. Then, we proved some geometric theorems about properties...

The present study was conducted to reveal the morphological characteristics of neurons nodose ganglion expressing nitric oxide synthase (NOS) and/or tyrosine hydroxylase (TH). NADPH-diaphorase activity, a histochemical marker for NOS, observed in 60% ganglionic neurons, but TH-immunoreactivity shared only 8% total neurons. In this ganglion, 55% Thimmunoreactive expressed NADPHdiaphorase activity. By triple combination retrograde neuronal tracer (rhodamine labeled latex microspheres) injected...

ABSTRACT We have previously shown that erythropoietin and receptor mRNAs are expressed in mouse embryos decidua at the early postimplantation stage, mRNA is advance of mRNA. subsequently studied role exogenous development until embryo proper can express by itself. In present study, to block signal decidual body where develops with decidua, we injected an antierythropoietin antibody or soluble into bodies through uterine wall day 6 gestation. For controls, saline denatured receptor. After 3 4...

The effects of neonatal vitamin A exposure on six behavioral parameters in adult Wistar rats were investigated. Newborn male injected intraperitoneally with 80, 000 IU/kg/day retinol palmitate (vitamin A) dissolved physiological saline or vehicle alone (controls) during 1-5 days after birth. animals examined using the open field test (at 36 weeks age), rotarod (38 weeks), Biel water-maze (40 conditioned avoidance (42 running-wheel activity pentobarbital challenge (44 and tail flick (45...

To examine the developmental toxicity of caffeine, Xenopus larvae just after hatching, were continuously exposed to 100-2,000 mg/L caffeine for 48 hours. Caffeine interfered with development at a concentration 100 and above in concentration-dependent manner. Characteristic external abnormalities, such as shortened body wavy fins, observed, severity which was clearly dependent. These frequently accompanied by abnormal flexure edema fin. Light microscopy revealed that exposure induced severe...

Abstract Cytoglobin (CYGB), a new member of the globin family, remains to be characterized. To assess its expression in human malignancies, we screened number cell lines originated from many tissues, and found that several, but not all, melanoma expressed CYGB mRNA protein at much higher levels than cells other origins. Melanocytes, primary origin melanoma, also high level. Bisulfite-modified genomic sequencing revealed several abrogated were epigenetically regulated by hypermethylation...

Summary 1. Dietary high protein intake in stroke‐prone spontaneously hypertensive rats (SHRSP) induces a reduction of blood pressure and decreases the incidence cerebral lesion. The objective this investigation was to determine effects nutritional factors dietary on reproduction, growth, pre‐weaning reflex development, postweaning behaviour learning performance SHRSP. 2. SHRSP were fed rat diet (OA2 diet) standard for (FSP diet). Rats mated by brother‐sister combinations observed...