A5004384834- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- HER2/EGFR in Cancer Research
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- Immune cells in cancer
- interferon and immune responses
- Estrogen and related hormone effects
- Ferroptosis and cancer prognosis
- Cancer-related Molecular Pathways
- Advanced Breast Cancer Therapies
- Cytokine Signaling Pathways and Interactions
- Muscle metabolism and nutrition
- Immunotherapy and Immune Responses
- Metal and Thin Film Mechanics
- Skin and Cellular Biology Research
- Fatty Acid Research and Health
- Wnt/β-catenin signaling in development and cancer
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Fibroblast Growth Factor Research
Institute of Cell Biology and Neurobiology
2021-2023
Center for Excellence in Molecular Cell Science
2023
National Research Council
2021
Istituti di Ricovero e Cura a Carattere Scientifico
2019
National Cancer Institute
2008-2010
Center for Cancer Research
2010
Biochemical Society
2008
University of Rome Tor Vergata
2006-2007
Metastatic disease is responsible for the majority of human cancer deaths. Understanding molecular mechanisms metastasis a major step in designing effective therapeutics. Here we show that T-box transcription factor Brachyury induces tumor cells epithelial-mesenchymal transition (EMT), an important progression primary tumors toward metastasis. Overexpression carcinoma induced changes characteristic EMT, including upregulation mesenchymal markers, downregulation epithelial and increase cell...
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease in need of prognostic markers to address therapeutic choices. We have previously shown that alternative splicing the actin regulator, hMENA, generates hMENA11a, and hMENAΔv6 isoforms with opposite roles cell invasion. examined expression pattern hMENA by immunohistochemistry, using anti-pan specific anti-hMENA11a antibodies, 285 PDACs, 15 PanINs, 10 pancreatitis, normal pancreas. Pan immunostaining, absent pancreas low-grade...
hMena and the epithelial specific isoform hMena11a are actin cytoskeleton regulatory proteins belonging to Ena/VASP family. EGF treatment of breast cancer cell lines upregulates hMena/hMena11a expression phosphorylates hMena11a, suggesting cross-talk between ErbB receptor family in cancer. The aim this study was determine whether overexpression cooperates with HER-2 signalling, thereby affecting HER2 mitogenic activity In a cohort tissue samples significant correlation among hMena,...
Background Understanding how cancer signaling pathways promote an immunosuppressive program which sustains acquired or primary resistance to immune checkpoint blockade (ICB) is a crucial step in improving immunotherapy efficacy. Among the that can affect ICB response interferon (IFN) pathway may be both detrimental and beneficial. The sensor retinoic acid-inducible gene I (RIG-I) induces IFN activation secretion activated by actin cytoskeleton disturbance. regulatory protein hMENA, along...
Employing purified extracellular matrix (ECM) proteins, i.e. type I, III, IV and V collagens (CI, CHI, CIV, CV), laminin (LM) fibronectin (FN), as antigen sources we detected autoantibodies to conformational and/or denatured ECM antigens among 34 of 50 sera obtained from Hashimoto's thyroiditis (HT) patients 6 51 control non-autoimmune thyroid disease healthy donors (HT vs. p=4×10 −9 ). Reactivity antigens, mostly due the IgG isotype, was observed in 30/50 HT 6/51 (p=3.5×10 −7 ) not always...
A typical feature in systemic lupus erythemathosus patients is the presence of autoantibodies to carboxyl-terminal homologous P proteins (P0, P1, P2) domain (C-22 P0 epitope). In this report we provide evidence for vivo immunogenicity protein head and neck cancer as well overexpression by immunohistochemistry C-22 epitope invasive carcinomas (55/57). Overexpression was also significantly associated with a number pathological lesions arising stratified epithelium including acanthosis (8/8),...
Targeting of immune checkpoint blockers (ICBs), such as cytotoxic T-lymphocyte antigen-4 and programmed-death 1/programmed-death ligand 1, has dramatically changed the landscape cancer treatment. Seeing patients who were refractory to conventional therapy recover after immunotherapy, with high rates objective durable responses increased overall survival, raised great enthusiasm in care research. However, date, only a restricted portion benefit from these therapies, due natural acquired...
Cardiomyocyte intercalated disks of delta-sarcoglycan-deficient cardiomyopathic hamsters (CMPHs) exhibit a pathological accumulation the N-cadherin/catenin complex. CMPHs fed with an alpha-linolenic acid (ALA)-enriched diet (CMPH/FS) display extended longevity compared to those standard (CMPH/PT) owing to, among others, amelioration both cardiac tissue structure and myocardial function. The present investigation was aimed at evaluating whether what extent ALA-enriched affects remodeling CMPH...
Human MENA(11a) (hMENA(11a)), an epithelial-associated isoform of the actin binding protein enabled homolog (ENAH, also known as mammalian ENA [MENA]), is upregulated and phosphorylated following activation human epidermal growth factor receptor (HER) 1, HER2, HER3. Here, we reveal a novel role this in sustaining cell survival propose hMENA(11a) marker HER3 resistance to phosphatidylinositol-3-kinase inhibition therapies.
Abstract Human Mena (hMENA), an actin regulatory protein of the ENA/VASP family, cooperates with ErbB receptor family signaling in breast cancer. It is overexpressed high-risk preneoplastic lesions and primary tumors where it correlates HER2 overexpression activated status AKT MAPK. The concomitant hMENA identifies cancer patients a worse prognosis. expressed along alternatively isoforms, hMENA11a hMENAΔv6 opposite functions. By Reverse Phase Protein Assay, we identified novel role for...
Abstract Background: The pancreatic ductal adenocarcinoma tumor microenvironment plays an important role in promoting the epithelial to mesenchymal transition (EMT), early event cancer, involved cancer invasiveness and progression. Among stromal components cancer-associated fibroblasts (CAFs) are responsible for peculiar known be linked induction of EMT. EMT process requires a dynamic remodeling actin cytoskeleton we have suggested that splicing program hMENA, regulator, play Two...
Abstract Background Epithelial to mesenchymal transition (EMT) is an early event in pancreatic cancer and has been involved invasiveness. An intense stromal reaction, peculiar the tumor microenvironment, includes cancer-associated fibroblasts(CAFs), abundant cells stroma, recently linked induction of EMT. On other hand, EMT process requires a dynamic remodeling actin cytoskeleton, splicing program hMENA, regulator actin, associated with process. We have described two alternatively expressed...
Abstract Background: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease with the worst survival rate among solid cancers. The pressing needs for extending life expectancy of patients are identification early prognostic markers and novel druggable pathways. PDAC arises generally from pancreatic intraepithelial neoplasia (PanIN) a dynamic interactions between tumor, stromal cells autocrine paracrine signaling lead to epithelial mesenchymal transition (EMT), process in natural...
Abstract Deciphering the complexity of tumor microenvironment (TME)is essential to unveil mechanisms therapy resistance and develop novel microenvironment-related anti-tumor treatment. Actin cytoskeleton dynamics act as platforms for gene regulation key signaling transduction pathways involved in cross-talk among cells cellular non-cellular components TME.The actin regulatory protein hMENA undergoes tissue specific splicing, generating two alternatively expressed isoforms hMENA11a hMENAΔv6...
Deciphering the complexity of tumor microenvironment (TME)is essential to unveil mechanisms therapy resistance and develop novel microenvironment-related anti-tumor treatment. Actin cytoskeleton dynamics act as platforms for gene regulation key signaling transduction pathways involved in cross-talk among cells cellular non-cellular components TME.The actin regulatory protein hMENA undergoes tissue specific splicing, generating two alternatively expressed isoforms hMENA11a hMENAΔv6 with a...