A5037090023- Estrogen and related hormone effects
- Retinoids in leukemia and cellular processes
- Gout, Hyperuricemia, Uric Acid
- Connexins and lens biology
- Heat shock proteins research
- Pharmacogenetics and Drug Metabolism
- Botanical Research and Applications
- Antioxidant Activity and Oxidative Stress
- Cytokine Signaling Pathways and Interactions
- Prostate Cancer Treatment and Research
- Nicotinic Acetylcholine Receptors Study
- Nitric Oxide and Endothelin Effects
- Mechanisms of cancer metastasis
- Cancer Cells and Metastasis
- Adenosine and Purinergic Signaling
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
- Axon Guidance and Neuronal Signaling
- Transplantation: Methods and Outcomes
- Pharmacology and Obesity Treatment
- Cystic Fibrosis Research Advances
- Chemokine receptors and signaling
- PI3K/AKT/mTOR signaling in cancer
- Immune Response and Inflammation
- Cancer, Lipids, and Metabolism
University of Palermo
2002-2004
Although estrogen receptors (ERs) are expressed in human hepatocellular carcinoma (HCC), several clinical trials have failed to demonstrate the efficacy of antiestrogen treatment HCC patients. Recently, identification ER splicing variants has enlightened complex nature signaling peripheral tissues; this may help understanding role either nontumoral or malignant nonclassical target organs, including liver. In work we investigated mRNA expression wild-type and splice ERα nontumoral, cirrhotic,...
Abstract We have investigated the effects of sex steroids, estradiol (E2), and testosterone (T) on synthesis tumor necrosis factor alpha (TNF‐α) interleukin‐10 (IL‐10) in phorbol‐myristate‐acetate (PMA)‐differentiated human monoblastic U937 cells. The ability both hormones to modulate viability programmed cell death macrophage‐like PMA‐differentiated cells was also inspected. E2 increased TNF‐α synthesis, whereas T had no effect production this cytokine. combination its antagonist tamoxifen...
In this study, we investigated androgen metabolism in two different human prostate cancer cell lines, the androgen-responsive LNCaP cells and nonresponsive PC3 cells. Following 24-h 72-h incubation with either testosterone (T) or androstenedione (Ad) used as precursor, divergent patterns rates of were observed. Given recent interest multiple uses embryonic adult stem for basic applied research, compared expression three presumptive markers (Oct-4, SUZ-12, Cripto-1), along connexin 43 (Cx43),...
Food supplementation with Opuntia ficus-indica (OFI) has been associated a significant reduction in total cholesterol, body fat, hyperglycemia and blood pressure. Since OFI may also have antioxidant anti-atherogenic properties, we hypothesized that its might reduce atherogenic lipoproteins, including small, dense low-density lipoproteins (sdLDL). Forty-nine patients (13 men 36 women, mean age: 56 ± 5 years) one or two criteria for the metabolic syndrome weekly consumed 500 g of pasta...
Abstract BACKGROUND Gap‐junction‐mediated intercellular communication (GJIC) is required for normal development and tissue homeostasis. However, the role of GJIC in human prostate carcinogenesis progression remains ill‐defined. METHODS The ability hormones, anti‐hormones, anti‐hypertensive drug, forskolin, to restore non‐tumorigenic (RWPE‐1 PWR‐1E) malignant (RWPE‐2, LNCaP, DU‐145) epithelial cell lines, was examined by Scrape‐Loading/Dye Transfer (SL/DT) Fluorescence Recovery After...
A bstract : Gap‐junction‐mediated intercellular communication (GJIC) is required for completion of embryonic development, tissue homeostasis, and regulation cell proliferation death. Although, as emphasized in several reports, defects or disruption GJIC may be important carcinogenesis, the potential role onset progression human prostate cancer remains ill‐defined. The gap junction channel‐forming connexins (Cx) comprise a multigene family highly conserved proteins that are differentially...
Xanthine oxidase (XO) plays an important role in various forms of ischemic and vascular injuries, inflammatory diseases chronic heart failure. The XO inhibitors allopurinol oxypurinol held considerable promise the treatment these conditions both experimental animals human clinical trials. More recently, endothelium-based protective effect sildenafil has been reported preconditioning prior to ischemia/reperfusion healthy subjects. Based on structural similarities between with zaprinast...
In this study we have investigated androgen (testosterone and androstenedione) metabolism in malignant HepG2, Huh-7, HA22T human liver cell lines. Following 72-h incubation with testosterone or androstenedione, estrogen formation through aromatase activity was consistently higher HepG2 cells (being nearly 100%) moderate Huh7 (34%), while it undetectable cells. The produced estrogens are completely conjugated by sulpho-transferase (EST) cells, 25% remains the free form Huh-7 show a markedly...
The seeming impairment of retinoid metabolism in human breast tumor cells has been attributed to the lower expression cellular retinol binding proteins (CRBPs), alcohol/retinol dehydrogenases, or aldehyde/retinaldehyde dehydrogenases. In a previous study we indicated that xanthine dehydrogenase (XDH) is able oxidize actively both all-trans-retinol (t-ROL) bound CRBP (holo-CRBP) and all-trans-retinaldehyde (t-RAL) all-trans-retinoic acid (t-RA) mammary epithelial (HMEC). Since XDH are...
A bstract : We have previously reported that gap junction‐mediated intercellular communication (GJIC) can be restored in junctionally deficient human prostate epithelial cells, also suggesting GJIC activity is regulated by estrogen. In the present work, we report studies on sex steroid regulation of and proliferative both nontumoral (Chang liver, CL) malignant (HepG2, Huh7) liver cells. Junctional cell growth were measured using scrape‐loading/dye‐transfer (SL/DT) MTS assay, respectively....
Abstract The retinoic acid deficiency in breast tumour epithelial cells has been ascribed to an insufficient expression of either the enzyme(s) involved its biosynthesis or cellular retinol binding protein (CRBP) both. In attempt define mechanisms underpinning these cell model systems, we have investigated potential regulatory effect oestrogen (17β‐estradiol) on one key player biosynthesis, xanthine dehydrogenase (XDH). This enzyme is consistently expressed and very active non‐malignant...
In this study we investigated the impact of estrogen antagonists and 16alpha-OHE1 (an derivative that binds to induces transactivation receptors) on metabolism in malignant HepG2 human liver cells featured by high sulfotransferase (EST); our aim was clarify potential correlation EST ER. As expected, exhibited a very activity, with majority metabolites (over 86%) being detected as sulfates 24 h. The coincubation E2 antiestrogen tamoxifen induced weak inhibition activity (from 85.4% 81.5%),...
Abstract Background: We investigated aromatase (Aro)-driven estrogen formation in non-tumoral and malignant liver tissues cells, also relation to expression of the receptors α β (ERα ERβ) amphiregulin (AREG), aiming gain insights into potential role estrogens human hepatocellular carcinoma (HCC). Materials methods: Chromatographic reverse transcriptase polymerase chain reaction (RT-PCR) analyses were used assess activity Aro enzyme AREG as well wild-type variant ERs, both vivo vitro....
Abstract It is recognized that the product of neurofibromatosis type 2 (NF2) tumor suppressor gene, also referred to as Merlin/neurofibromin, implicated in homeostatic regulation liver stem cell niche through control abundance and signaling epidermal growth factor receptor (EGFR), with a mechanism independent Mst/Lats/Yap Hippo pathway [1]. We have previously reported locally elevated estrogen formation, driven by abnormally high expression function aromatase enzyme, may be both development...
Retinoic acid is regarded as the retinol metabolite that controls proliferation and differentiation of epithelial cells. In present study, we investigated potential role xanthine dehydrogenase (XDH) in retinoic biosynthesis human thyroid glandular cells (HTGC). particular, observed cellular retinoids binding proteins (CRBPs) are also implicated biosynthetic pathway leading to formation primary cultures HTGC, have already reported for mammary (HMEC). After partial protein purification, enzyme...
Several studies suggest that xanthine dehydrogenase (XDH) and its oxidase form (XO) play an important role in various types of ischemic vascular injuries. Recently, we have demonstrated estradiol (E2) induces a significant decrease the expression activity XDH conversion to XO human mammary epithelial cells. E2 is known induce upregulation eNOS gene aortic endothelial Because XO-derived O2·- combines with ·NO yield ONOO-, considering ONOO- converts XO, resulting increase reactive oxygen...
Abstract Although there is evidence that hints at a potential role of estrogen in hepatocellular carcinoma (HCC), the use antiestrogens has failed to improve survival patients. We have investigated aromatase-driven formation nontumoral and malignant human liver tissues cells, also relation expression receptor (ER) α, ERβ their splicing variants amphiregulin (AREG), an EGFR ligand, aiming get insights underlying mechanism(s) HCC. Chromatographic exon-specific RT-PCR analyses were used assess...
Abstract There is evidence that hints at a potential role of sex steroids in development and progression human hepatocellular carcinoma (HCC). Previous studies have revealed estrogen receptors (ER) are expressed primary HCC. However, the use antiestrogens has failed to improve disease-free overall survival patients. In present study we investigated aromatase-driven formation nontumoral malignant liver tissues cells, also relation expression ERα, ERβ, their splicing variants, aiming get...
Abstract Although there is evidence that hints at a potential role of estrogen in hepatocellular carcinoma (HCC), the use antiestrogens has failed to improve disease-free and overall survival HCC patients. In present study we have investigated aromatase-driven formation nontumoral malignant human liver tissues cells, also relation expression receptor (ER) β, ERα, their splicing variants, aiming get insights into estrogens underlying mechanism(s) HCC. Chromatographic exon-specific RT-PCR...