A5060924890- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Biochemical and Structural Characterization
- Viral Infectious Diseases and Gene Expression in Insects
- Retinal Development and Disorders
- Biotin and Related Studies
- Caveolin-1 and cellular processes
- Enzyme Production and Characterization
- Protein Kinase Regulation and GTPase Signaling
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Click Chemistry and Applications
- CRISPR and Genetic Engineering
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Electrochemical sensors and biosensors
- Lipid Membrane Structure and Behavior
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Advanced Biosensing Techniques and Applications
- Trypanosoma species research and implications
Queensland University of Technology
2019-2025
Commonwealth Scientific and Industrial Research Organisation
2019-2024
ARC Centre of Excellence in Synthetic Biology
2019-2024
Personalis (United States)
2024
Saint Petersburg State Pediatric Medical University
2024
ACT Government
2024
Ministry of Health of the Russian Federation
2024
Space Research Institute
2023
Australian Research Council
2022-2023
The University of Queensland
2012-2021
Signaling from JAK (Janus kinase) protein kinases to STAT (signal transducers and activators of transcription) transcription factors is key many aspects biology medicine, yet the mechanism by which cytokine receptors initiate signaling enigmatic. We present a complete mechanistic model for activation receptor-bound JAK2, based on an archetypal receptor, growth hormone receptor. For this, we used fluorescence resonance energy transfer monitor positioning JAK2 binding motif in receptor dimer,...
The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and emerging as powerful tool for isolation cellular engineering of fusions in many different fields biological research. Using X-ray crystallography isothermal titration calorimetry, we determine the molecular details GFP:GFP-nanobody complex formation explain basis high affinity at same time specificity binding. Although GFP-nanobody can also bind YFP, it...
Rab proteins comprise a family of small GTPases that serve regulatory role in membrane traffic. These are part cytosolic and associated with the membranes specific exocytic endocytic organelles. Smg p25A/rab3A GDI, protein which inhibits dissociation GDP from smg p25A/rab3A, Sec4p, rab11, has also been found to prevent association rab3A membrane. In this study, we have used Madin-Darby canine kidney cells permeabilized bacterial toxin streptolysin O test general activity GDI modulating...
Rab/Ypt guanosine triphosphatases (GTPases) represent a family of key membrane traffic regulators in eukaryotic cells whose function is governed by the diphosphate (GDP) dissociation inhibitor (RabGDI). Using combination chemical synthesis and protein engineering, we generated crystallized monoprenylated Ypt1:RabGDI complex. The structure complex was solved to 1.5 angstrom resolution provides structural basis for ability RabGDI inhibit release nucleotide Rab proteins. Isoprenoid binding...
The study of the various protein kinases reveals that, despite their considerably diversity, they have evolved from a common origin. Eleven conserved subdomains been described that encompass catalytic core these enzymes. One regions, subdomain II, contains an invariant lysine residue present in all known kinase domains. Two facts suggested this II is essential for binding ATP: (i) several investigators demonstrated physically proximal to ATP molecule, and (ii) conservative substitutions at...
In mammalian cells three closely related cavin proteins cooperate with the scaffolding protein caveolin to form membrane invaginations known as caveolae. Here we have developed a novel single-molecule fluorescence approach directly observe interactions and stoichiometries in complexes from cell extracts vitro synthesized components. We show that up 50 cavins associate on caveola. However, rather than forming single coat complex containing family members, analysis reveals an exquisite...
The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has emphasized the vulnerability of human populations to novel viral pressures, despite vast array epidemiological and biomedical tools now available. Notably, modern genomes contain evolutionary information tracing back tens thousands years, which may help identify viruses that have impacted our ancestors-pointing future potential. Here, we apply analyses genomic datasets recover selection events involving...
Mild attachment: Proteins can be ligated chemoselectively and immobilized on functionalized glass surfaces by means of Diels–Alder cycloadditions (see scheme).
A sticky situation: site-specific immobilization of proteins onto a glass surface may allow the creation protein microarrays. This approach relies on Staudinger ligation between azide-modified and phosphane-functionalized surface. Supporting information for this article is available WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z502057_s.pdf or from author. Please note: The publisher not responsible content functionality any supporting supplied by authors. Any queries (other than...
Prenylated Rab GTPases regulate intracellular vesicle trafficking in eukaryotic cells by associating with specific membranes and recruiting a multitude of Rab-specific effector proteins. Prenylation, membrane delivery, recycling all 60 members the GTPase family are regulated two related molecules, escort protein (REP) GDP dissociation inhibitor (GDI). Biophysical analysis interaction prenylated proteins is complicated their low solubility aqueous solutions. Here, we used expressed ligation...
Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable "CAAX-box" tetrapeptide sequence (A is aliphatic) at C terminus of protein and surface-exposed thiols (see scheme). This method enables oriented covalent immobilization proteins directly from expression lysates without additional purification or derivatization steps. Detailed facts importance specialist readers are published...
Choroideremia (CHM) is an X- linked retinal degeneration that symptomatic in the 1st or 2nd decade of life causing nyctalopia and loss peripheral vision. The disease progresses through mid-life, when most patients become blind. CHM a favorable target for gene augmentation therapy, as due to function protein necessary cell health, Rab Escort Protein 1 (REP1).The cDNA can be packaged recombinant adeno-associated virus (rAAV), which has established track record human therapy studies, and,...