A5090190690- Colorectal Cancer Treatments and Studies
- Estrogen and related hormone effects
- Genetic factors in colorectal cancer
- Epigenetics and DNA Methylation
- Advanced Breast Cancer Therapies
- Cancer Genomics and Diagnostics
- Cancer, Lipids, and Metabolism
- BRCA gene mutations in cancer
- Ovarian cancer diagnosis and treatment
- Radiomics and Machine Learning in Medical Imaging
- Breast Cancer Treatment Studies
- Cellular transport and secretion
- Colorectal Cancer Surgical Treatments
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Melanoma and MAPK Pathways
- Ferroptosis and cancer prognosis
- PARP inhibition in cancer therapy
- Colorectal Cancer Screening and Detection
- Gene expression and cancer classification
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer-related Molecular Pathways
- Glutathione Transferases and Polymorphisms
- Thyroid Cancer Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
Universidad de Granada
2019-2023
Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research
2019-2023
Diazyme (United States)
2004-2023
Eli Lilly (United States)
2023
Agendia (Netherlands)
2009-2017
The Netherlands Cancer Institute
2010-2016
Radboud University Nijmegen
2016
University Medical Center
2013-2016
Radboud University Medical Center
2016
Dutch Cancer Society
2016
Purpose This study aims to develop a robust gene expression classifier that can predict disease relapse in patients with early-stage colorectal cancer (CRC). Patients and Methods Fresh frozen tumor tissue from 188 stage I IV CRC undergoing surgery was analyzed using Agilent 44K oligonucleotide arrays. Median follow-up time 65.1 months, the majority of (83.6%) did not receive adjuvant chemotherapy. A nearest mean developed cross-validation procedure score all genes for their association...
In most colorectal cancer (CRC) patients, outcome cannot be predicted because tumors with similar clinicopathological features can have differences in disease progression and treatment response. Therefore, a better understanding of the CRC biology is required to identify those patients who will benefit from chemotherapy find more tailored therapy plan for other patients. Based on unsupervised classification whole genome data 188 stages I–IV molecular was developed that consist at least three...
Abstract Purpose: Mutation of BRAF at the valine 600 residue occurs in approximately 10% colorectal cancers, a group with particularly poor prognosis. The response mutant cancer to recent targeted strategies such as anti-BRAF or combinations MEK and EGFR inhibitors remains limited highly heterogeneous within V600E cohorts. There is clearly an unmet need understanding biology cancers potential subgroups this population. Experimental Design: In biggest yet reported cohort 218 gene expression...
Mannose 6-phosphate receptors (MPRs) deliver lysosomal hydrolases from the Golgi to endosomes and then return complex. TIP47 recognizes cytoplasmic domains of MPRs is required for endosome-to-Golgi transport. Here we show that also bound directly Rab9 guanosine triphosphatase (GTPase) in its active, GTP-bound conformation. Moreover, increased affinity cargo. A functional binding site was stimulation MPR transport vivo. Thus, a cytosolic cargo selection device may be selectively recruited...
Using cDNA database mining strategies and real-time quantitative reverse transcription-PCR, we identified B7-H4 as a novel gene that is overexpressed in ovarian breast cancer tissues when compared with normal tissues. The encodes protein of 282 amino acids signal sequence, an immunoglobulin domain, COOH-terminal hydrophobic transmembrane domain. Immunohistochemistry experiments show plasma membrane staining serous cancer, confirming the tissue specificity cell surface localization. We have...
Abstract Invasive lobular carcinoma (ILC) is the second most frequently occurring histological breast cancer subtype after invasive ductal (IDC), accounting for around 10% of all cancers. The molecular processes that drive development ILC are still largely unknown. We have performed a comprehensive genomic, transcriptomic and proteomic analysis large patient cohort present here an integrated portrait ILC. Mutations in CDH1 PI3K pathway frequent alterations identified two main subtypes ILCs:...
Background and Methods Formalin Fixed Paraffin Embedded (FFPE) samples represent a valuable resource for cancer research. However, the discovery development of new biomarkers often requires fresh frozen (FF) samples. Recently, Whole Genome (WG) DASL (cDNA-mediated Annealing, Selection, extension Ligation) assay was specifically developed to profile FFPE tissue. thorough comparison data generated from RNA Fresh Frozen using this platform is lacking. To end we profiled, in duplicate, 20...
The transforming growth factor β (TGF-β) pathway acts as a double-edged sword in tumorigenesis. By constraining epithelial cell growth, TGF-β is potent tumor suppressor. However, also key player the induction of epithelial-to-mesenchymal transition (EMT), thereby enhancing invasiveness and metastasis. Furthermore, signaling has recently been correlated with resistance against both targeted conventional anticancer agents. Here, we present data demonstrating role for chemotherapy colorectal...
<h3>Objective</h3> To develop gene expression profiles that characterise <i>KRAS</i>-, <i>BRAF</i>- or <i>PIK3CA</i>-activated- tumours, and to explore whether these might be helpful in predicting the response epidermal growth factor receptor (EGFR) pathway inhibitors better than mutation status alone. <h3>Design</h3> Fresh frozen tumour samples from 381 colorectal cancer (CRC) patients were collected mutations <i>KRAS</i>, <i>BRAF</i> <i>PIK3CA</i> assessed. Using microarray data, three...
Approximately 20% of patients with stage II colorectal cancer will experience a relapse. Current clinical-pathologic stratification factors do not allow clear identification these high-risk patients. ColoPrint (Agendia, Amsterdam, The Netherlands, http://www.agendia.com) is gene expression classifier that distinguishes low or high risk disease relapse.ColoPrint was developed using whole-genome data and validated in several independent validation cohorts. Stage from studies were pooled (n =...
The aim of this study was to independently validate a genomic signature developed both assess recurrence risk in stage II patients and assist treatment decisions.Adjuvant therapy is recommended for high-risk with colon cancer, but better tools the patients' prognosis accurately are still required.Previously, an 18-gene had been validated on independent cohort, using full genome microarrays. In study, gene translated as robust diagnostic test (ColoPrint), customized 8-pack arrays. addition,...
Background: Platelets are active players in tumorigenesis, although the exact interactive mechanisms and their direct impact on tumor cells remain largely unknown.Methods: Bidirectional transference of lipids, proteins RNA between platelets its cell behavior process analyzed this work.Phenotypic, genetic functional modifications induced by were both lines circulating (CTCs).Results: Data from these assays showed that transferred structural components to with higher efficiency than (p =...
Currently, there are no effective biomarkers for ovarian cancer prognosis or prediction of therapeutic response. The objective this study was to examine a panel 10 serum biochemical parameters their ability predict response chemotherapy, progression and survival patients. Sera from patients were collected prior during chemotherapy analysed by enzyme-linked immunosorbent assay CA125, kallikreins 5, 6, 7, 8, 11, B7-H4, regenerating protein IV Spondin-2. odds ratio hazard 95% confidence...
Abstract Aromatase inhibitors are the major first-line treatment of estrogen receptor–positive breast cancer, but resistance to is common. To date, no biomarkers have been validated clinically guide subsequent therapy in these patients. In this study, we mapped genome-wide chromatin-binding profiles receptor α (ERα), along with epigenetic modifications H3K4me3 and H3K27me3, that responsible for determining gene transcription (n = 12). Differential binding patterns ERα, H3K4me3, H3K27me3 were...
Triple negative (TN) breast cancers make up some 15% of all cancers. Approximately 10–15% are mutant for the tumor suppressor, BRCA1. BRCA1 is required homologous recombination‐mediated DNA repair and deficiency results in genomic instability. BRCA1‐mutated tumors have a specific pattern copy number aberrations that can be used to classify as BRCA1‐like or non‐BRCA1‐like. mutation, promoter methylation, status genome‐wide expression data was determined 112 TN cancer samples with long‐term...
Patients with BRCA1-like tumors correlate improved response to DNA double-strand break-inducing therapy. A gene expression-based classifier was developed distinguish between and non-BRCA1-like tumors. We hypothesized that these may also be more sensitive PARP inhibitors than standard treatments. diagnostic expression signature (BRCA1ness) using a centroid model 128 triple-negative breast cancer samples from the EU FP7 RATHER project. This BRCA1ness then tested in HER2-negative patients (n =...
We describe here the kinetics of interaction GTP and GDP with small GTP-binding proteins Rab5 Rab7. It was possible to make use intrinsic fluorescence these proteins, since contains two Rab7 three tryptophan residues, respectively. With both enzymes, there is a significant decrease in on binding an increase GDP. As protein Ha-Ras p21 EF-Tu, nucleotide occurs at least steps describable terms relatively weak initial followed by highly irreversible isomerization protein-nucleotide complex,...
Abstract Microsatellite instability ( MSI ) occurs in 10–20% of colorectal tumours and is associated with good prognosis. Here we describe the development validation a genomic signature that identifies cancer patients caused by DNA mismatch repair deficiency high accuracy. status for 276 stage II III has been determined. Full‐genome expression data was used to identify genes correlate status. A subset these samples n = 73) had sequencing 615 available. An gene 64 developed validated two...