A5072935853- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Caveolin-1 and cellular processes
- Microtubule and mitosis dynamics
- Tissue Engineering and Regenerative Medicine
- Wnt/β-catenin signaling in development and cancer
- Fibroblast Growth Factor Research
- Cell Adhesion Molecules Research
- Connective tissue disorders research
- Neuroscience and Neuropharmacology Research
- Hippo pathway signaling and YAP/TAZ
- Force Microscopy Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Molecular Communication and Nanonetworks
- Erythrocyte Function and Pathophysiology
- Electrospun Nanofibers in Biomedical Applications
- Micro and Nano Robotics
Nara Institute of Science and Technology
2021-2025
The University of Queensland
2019-2023
To move forward, migrating cells must exert backward forces against the extracellular environment. Recent studies have highlighted importance of integrin-independent for cell migration; but molecular machinery that exerts remains unclear. Here, we show clutch-linker molecule shootin1 and adhesion L1 transmit force treadmilling actin filaments to adhesive environment rapid dendritic migration. Notably, weak traction forces, ~100 times weaker than integrin-based by constituting an slippery...
Dendritic spines constitute the major compartments of excitatory post-synapses. They undergo activity-dependent enlargement, which is thought to increase synaptic efficacy underlying learning and memory. The spine enlargement requires activation signaling pathways leading promotion actin polymerization within spines. However, molecular machinery that suffices for structural plasticity remains unclear. Here, we demonstrate shootin1a links polymerizing filaments in with cell-adhesion molecules...
Epithelial networks are commonly generated by processes where multicellular aggregates elongate and branch. Here, we focus on understanding cellular mechanisms for elongation using an organotypic culture system as a model of mammary epithelial anlage. Isotropic cell broke symmetry slowly elongated when transplanted into collagen 1 gels. The elongating regions displayed enhanced proliferation that was necessary to occur. Strikingly, this locoregional increase in occurred fibrils reorganized...
Background Information Epithelial collective cell migration requires the intrinsic locomotor activity of cells to be coordinated across populations. This coordination is governed by presence cell–cell adhesions as well cooperative behaviour within monolayer. Results Here, we report a role for Caveolin‐1 (CAV1) in epithelial migration. CAV1 depletion reduced migratory AML12 liver when grown monolayers, but not individual cells. suggested that component process which multicellular collectivity...
SUMMARY Actin dynamics mediate cell morphogenesis. filaments polymerize outward at protrusions such as the leading edge of migrating cells, thereby pushing membrane to protrude. The current paradigm explains that actin are regulated by signaling. However, it is unclear how cells spontaneously form actin-based even without a specific local signaling cue. We found arrays treadmilling emerge widely in and move direction polymerization waves. Their arrival periphery pushes plasma Furthermore,...
Abstract Mechanical tension governs epithelial morphogenesis and homeostasis, but its regulation remains poorly understood. Tension is commonly contractile, arising when the actomyosin cortices of cells are mechanically coupled together by cadherin adhesion. Here we report that caveolae control levels show this necessary for oncogene-transfected to be eliminated apical extrusion. Depletion caveolin-1 (CAV1) in surrounding epithelium, not oncogene-expressing cells, blocked extrusion leading...
Extracellular matrix (ECM) provides fundamental support for epithelial tissues and controls cell function. The chemistry mechanical properties of ECM components, including stiffness, elasticity, fibrillar organization, influence tissue responses. Here we present a protocol describing the culture transfer acini from Matrigel to collagen gel an approach axially align fibrils by external stretching. This uses MCF10A cells needs be modified different lines. For complete details on use execution...
Abstract Epithelial networks are commonly generated by processes where multicellular aggregates elongate and branch. Here we focus on understanding cellular mechanisms for elongation, using an organotypic culture system as a model of mammary epithelial anlage. Isotropic cell broke symmetry slowly elongated when transplanted into collagen 1 gels. The elongating regions displayed enhanced proliferation that was necessary elongation to occur. Strikingly, this loco-regional increase in occurred...