A5061297925- Geophysical and Geoelectrical Methods
- Caveolin-1 and cellular processes
- Geophysical Methods and Applications
- Seismic Waves and Analysis
- Landslides and related hazards
- Microtubule and mitosis dynamics
- Neurogenesis and neuroplasticity mechanisms
- Cellular transport and secretion
- Genetics, Aging, and Longevity in Model Organisms
- Photosynthetic Processes and Mechanisms
- Remote Sensing and LiDAR Applications
- Aeolian processes and effects
- Soil erosion and sediment transport
- Zebrafish Biomedical Research Applications
- 3D Surveying and Cultural Heritage
- DNA Repair Mechanisms
- Sarcoma Diagnosis and Treatment
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Lipid Membrane Structure and Behavior
- Coastal wetland ecosystem dynamics
- Geological formations and processes
- Erythrocyte Function and Pathophysiology
Karolinska Institutet
2009-2024
British Geological Survey
2012-2024
Oak Ridge National Laboratory
2023
Idaho State University
2020-2022
University of Michigan
2021
Natural Environment Research Council
2017
The University of Queensland
2005-2010
Max Planck Institute of Molecular Cell Biology and Genetics
1999-2003
Max Planck Institut für Zellbiologie
1999-2001
Max Planck Society
2001
We have investigated the role of cytoplasmic dynein in microtubule organizing center (MTOC) positioning using RNA-mediated interference (RNAi) Caenorhabditis elegans to deplete product heavy chain gene dhc-1. Analysis with time-lapse differential contrast microscopy and indirect immunofluorescence revealed that pronuclear migration centrosome separation failed one cell stage dhc-1 embryos. These phenotypes were also observed when dynactin components p50/dynamitin or p150Glued depleted RNAi....
In all eukaryotes, segregation of mitotic chromosomes requires their interaction with spindle microtubules. To dissect this interaction, we use live and fixed assays in the one-cell stage Caenorhabditis elegans embryo. We compare consequences depleting homologues centromeric histone CENP-A, kinetochore structural component CENP-C, chromosomal passenger protein INCENP. Depletion either CeCENP-A or CeCENP-C results an identical “kinetochore null” phenotype, characterized by complete failure...
Using quantitative light microscopy and a modified immunoelectron microscopic technique, we have characterized the entry pathway of cholera toxin binding subunit (CTB) in primary embryonic fibroblasts. CTB trafficking to Golgi complex was identical caveolin-1null (Cav1−/−) mouse fibroblasts (MEFs) wild-type (WT) MEFs. Cav1−/− MEFs predominantly clathrin dynamin independent but relatively cholesterol dependent. Immunoelectron used quantify budded surface-connected caveolae identify...
Centrosomes mature as cells enter mitosis, accumulating γ-tubulin and other pericentriolar material (PCM) components. This occurs concomitant with an increase in the number of centrosomally organized microtubules (MTs). Here, we use RNA-mediated interference (RNAi) to examine role aurora-A kinase, AIR-1, during centrosome maturation Caenorhabditis elegans. In air-1(RNAi) embryos, centrosomes separate normally, event that before C. After nuclear envelope breakdown, separated collapse...
Although the importance of clathrin- and caveolin-independent endocytic pathways has recently emerged, key aspects these routes remain unknown. Using quantitative ultrastructural approaches, we show that clathrin-independent carriers (CLICs) account for approximately three times volume internalized by clathrin-mediated pathway, forming major pathway involved in uptake fluid bulk membrane fibroblasts. Electron tomographic analysis 3D morphology earliest shows they are multidomain organelles...
γ-Tubulin–containing complexes are thought to nucleate and anchor centrosomal microtubules (MTs). Surprisingly, a recent study (Strome, S., J. Powers, M. Dunn, K. Reese, C.J. Malone, White, G. Seydoux, W. Saxton. Mol. Biol. Cell. 12:1751–1764) showed that asters form in Caenorhabditis elegans embryos depleted of γ-tubulin by RNA-mediated interference (RNAi). Here, we investigate the nucleation organization MT C. severely compromised for function. We characterize ∼98% RNAi, expressing mutant...
ABSTRACT The internal moisture dynamics of an aged (> 100 years old) railway earthwork embankment, which is still in use, are investigated using 2D and 3D resistivity monitoring. A methodology was employed that included automated ERT data capture telemetric transfer with on‐site power generation, the correction models for seasonal temperature changes translation subsurface distributions into content based on petrophysical relationships developed embankment material. Visualization as...
Systems and methods are described for monitoring temporal spatial moisture content changes in clay embankments using electrical resistivity tomography (ERT) imaging. The methodology is based upon development of a robust relationship between fill its use the transformation image differences terms relative changes. Moisture level movement applications exemplified two case histories from UK. first BIONICS embankment, near Newcastle (NE England), which was constructed 2005 varying degrees...
Caveolae are an abundant feature of mammalian cells. Integral membrane proteins called caveolins drive the formation caveolae but precise mechanisms underlying caveola formation, and origin during evolution, unknown. Systematic evolutionary analysis shows conservation genes encoding in metazoans. We provide evidence for extensive ancient, local genomic gene duplication, classify distinct caveolin families. Vertebrate caveolin-1 caveolin-3 isoforms, as well invertebrate (Apis mellifera,...
In contrast to mammals, salamanders and teleost fishes can efficiently repair the adult brain. It has been hypothesised that constitutively active neurogenic niches are a prerequisite for extensive neuronal regeneration capacity. Here, we show highly regenerative salamander, red spotted newt, displays an unexpectedly similar distribution of germinal with mammals under normal physiological conditions. Proliferation zones in newt brain restricted forebrain, whereas all other regions...
Abstract Quantifying the extent of soil erosion at a fine spatial resolution can be time consuming and costly; however, proximal remote sensing approaches to collect topographic data present an emerging alternative for quantifying volumes lost via erosion. Herein we compare terrestrial laser scanning (TLS), both unmanned aerial vehicle (UAV) ground photography (GP) structure‐from‐motion (SfM) derived topography. We cost‐effectiveness accuracy SfM techniques TLS gully surveying in upland...
Salamanders have a remarkable capacity to regenerate complex tissues, such as limbs and brain, are therefore an important comparative model system for regenerative medicine. Despite these unique properties among adult vertebrates, the genomic information amphibians in general, salamanders particular, is scarce. Here, we used massive parallel sequencing reconstruct de novo reference transcriptome of red spotted newt (Notophthalmus viridescens) containing 118,893 transcripts with N50 length...
Landslides in the Thompson River Valley, British Columbia, Canada, threaten serviceability of two railway lines that connect Vancouver to rest Canada and US. To minimise impact slope instability on vital transport infrastructure, as well terrestrial aquatic ecosystems, public safety, communities, local heritage, economy, better inform decision making, there is a need for monitoring. Since 2013, Ripley Landslide – small, slow-moving, translational landslide has been focus monitoring efforts...
The adult newt brain has a marked neurogenic potential and is highly regenerative. Ventricular, radial glia-like ependymoglia cells give rise to neurons both during normal homeostasis after injury, but subpopulations among have not been defined. We show here that substantial portion of GFAP+ in the proliferative hot spots telencephalon transit-amplifying characteristics. In contrast, proliferating cells, which are scattered along ventricular wall, stem cell features terms label retention...