Nicolas Jacquelot
A5029171391- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Eosinophilic Esophagitis
- T-cell and B-cell Immunology
- Eosinophilic Disorders and Syndromes
- Immune cells in cancer
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Asthma and respiratory diseases
- Gene Regulatory Network Analysis
- Circadian rhythm and melatonin
- Clostridium difficile and Clostridium perfringens research
- Dietary Effects on Health
- Diabetes and associated disorders
- Autoimmune and Inflammatory Disorders Research
- Psychological and Temporal Perspectives Research
- Autophagy in Disease and Therapy
- Reproductive System and Pregnancy
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Phagocytosis and Immune Regulation
- Monoclonal and Polyclonal Antibodies Research
Princess Margaret Cancer Centre
2021-2025
University of Calgary
2023-2024
Walter and Eliza Hall Institute of Medical Research
2018-2023
Institute of Cancer Research
2023
University Health Network
2021-2022
University of Toronto
2022
The University of Melbourne
2018-2021
Institut Gustave Roussy
2013-2020
Inserm
2013-2019
Université Paris-Saclay
2013-2017
Immune checkpoint inhibitors (ICIs) targeting the PD-1/PD-L1 axis induce sustained clinical responses in a sizable minority of cancer patients. We found that primary resistance to ICIs can be attributed abnormal gut microbiome composition. Antibiotics inhibited benefit patients with advanced cancer. Fecal microbiota transplantation (FMT) from who responded into germ-free or antibiotic-treated mice ameliorated antitumor effects PD-1 blockade, whereas FMT nonresponding failed do so....
Gut microbes affect immunotherapy The unleashing of antitumor T cell responses has ushered in a new era cancer treatment. Although these therapies can cause dramatic tumor regressions some patients, many patients inexplicably see no benefit. Mice have been used two studies to investigate what might be happening. Specific members the gut microbiota influence efficacy this type (see Perspective by Snyder et al. ). Vétizou found that optimal anticytotoxic lymphocyte antigen blockade required...
Abstract Despite successes, thus far, a significant proportion of the patients treated with anti-PD1 antibodies have failed to respond. We use mouse tumor models sensitivity and resistance flow cytometry assess tumor-infiltrating immune cells immediately after therapy. demonstrate that expression levels T-cell PD1 (PD1lo), myeloid, PDL1 (PDL1hi) in microenvironment inversely correlate dictate efficacy mAb function intratumor CD8+ T cells. In sensitive tumors, we reveal threshold for...
PD-1 blockade represents a major therapeutic avenue in anticancer immunotherapy. Delineating mechanisms of secondary resistance to this strategy is increasingly important. Here, we identified the deleterious role signaling via type I interferon (IFN) receptor tumor and antigen presenting cells, that induced expression nitric oxide synthase 2 (NOS2), associated with intratumor accumulation regulatory T cells (Treg) myeloid acquired anti-PD-1 monoclonal antibody (mAb). Sustained IFNβ...
Immune checkpoint blockers (ICB) have become pivotal therapies in the clinical armamentarium against metastatic melanoma (MMel). Given frequency of immune related adverse events and increasing use ICB, predictors response to CTLA-4 and/or PD-1 blockade represent unmet needs. Using a systems biology-based approach an assessment 779 paired blood tumor markers 37 stage III MMel patients, we analyzed association between parameters functional reactivity tumor-infiltrating cells after ex vivo...
The analysis of tumor growth curves is standard practice in experimental oncology including immunology. In oncology, cancer cells are inoculated into rodents (mostly mice) and their monitored by measuring diameter, surface or volume over time as a function distinct treatments. Then, different groups tumors/treatments compared among each other for evolution possible responses to treatment. R package TumGrowth has been created software tool allowing carry out series statistical comparisons...
Abstract The recognition of the immune system as a key component tumor microenvironment (TME) led to promising therapeutics. Because such therapies benefit only subsets patients, understanding activities cells in TME is required. Eosinophils are an integral part especially mucosal tumors. Nonetheless, their role and environmental cues that direct largely unknown. We report breast cancer lung metastases characterized by resident recruited eosinophils. Eosinophil recruitment metastatic sites...
Abstract Melanomas are characterized by high metastatic potential, with regional lymph node representing the most frequent site of early dissemination in this disease. These nodes also represent primary for differentiation natural killer (NK) cells. Although blood-derived NK cells can efficiently lyse melanoma isolated from (M-LN), there has been no study properties disease-relevant M-LN patients melanoma. Here, we report that contains 0.5% to 11% CD56bright among CD45+ hematopoietic present...
Melanoma prognosis is dictated by tumor-infiltrating lymphocytes, the migratory and functional behavior of which guided chemokine or cytokine gradients. Here, we retrospectively analyzed expression patterns 9 homing receptors (CCR/CXCR) in naive memory CD4+ CD8+ T lymphocytes 57 patients with metastatic melanoma (MMel) various sites metastases to evaluate whether cell CCR/CXCR correlates intratumoral accumulation, progression, and/or overall survival (OS). Homing receptor on strongly...
Interleukin-(IL) 22 production by intestinal group 3 innate lymphoid cells (ILC3) is critical to maintain gut homeostasis. However, IL-22 needs be tightly controlled; reduced expression associated with epithelial barrier defect while its overexpression promotes tumor development. Here, using a single-cell ribonucleic acid sequencing approach, we identified core set of genes increased ILC3. Among these genes, programmed cell death 1 (PD-1), extensively studied in the context cancer and...
Chronic inflammation of the gastrointestinal (GI) tract contributes to colorectal cancer (CRC) progression. While role adaptive T cells in CRC is now well established, innate immune cells, specifically lymphoid (ILCs), not understood. To define ILCs we employed complementary heterotopic and chemically-induced mouse models. We discovered that were abundant tumours contributed anti-tumour immunity. focused on ILC2 showed ILC2-deficient mice developed a higher tumour burden compared with...
Immunotherapies have had unprecedented success in the treatment of multiple cancer types, albeit with variable response rates. Unraveling complex network immune cells within tumor microenvironment (TME) may provide additional insights to enhance antitumor immunity and improve clinical response. Many studies shown that NK or innate lymphoid (ILC) regulatory capacity. Here, we identified CD103 as a marker was found on CD56+ were associated poor proliferative capacity tumor-infiltrating...
<div>Abstract<p>Immunotherapies have had unprecedented success in the treatment of multiple cancer types, albeit with variable response rates. Unraveling complex network immune cells within tumor microenvironment (TME) may provide additional insights to enhance antitumor immunity and improve clinical response. Many studies shown that NK or innate lymphoid (ILC) regulatory capacity. Here, we identified CD103 as a marker was found on CD56<sup>+</sup> were associated...
<p>Fig. S10. CD103+Lin- ILC1-like cells are associated with altered immune landscape within the TME.</p>
<p>Fig. S5. Identifying cellular clusters from scRNA-seq of ovarian tumours.</p>