María Paula Roberti
A5104752615- Cancer Immunotherapy and Biomarkers
- Gut microbiota and health
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Immune cells in cancer
- CAR-T cell therapy research
- Pancreatic and Hepatic Oncology Research
- Microscopic Colitis
- Trace Elements in Health
- Colorectal Cancer Surgical Treatments
- Cutaneous lymphoproliferative disorders research
- Cancer Research and Treatments
- Colorectal Cancer Treatments and Studies
- Drug Transport and Resistance Mechanisms
- Tryptophan and brain disorders
- Iron Metabolism and Disorders
- Clostridium difficile and Clostridium perfringens research
- Cancer, Stress, Anesthesia, and Immune Response
- Inflammatory Biomarkers in Disease Prognosis
- Chemokine receptors and signaling
German Cancer Research Center
2020-2025
Heidelberg University
2021-2025
National Center for Tumor Diseases
2021-2025
University Hospital Heidelberg
2021-2025
Institut Gustave Roussy
2016-2023
Inserm
2016-2023
La Ligue Contre le Cancer
2017-2023
DKFZ-ZMBH Alliance
2020
Fundación para la Investigación, Docencia y Prevención del Cáncer
2011-2018
CIUDAD
2012
Immune checkpoint inhibitors (ICIs) targeting the PD-1/PD-L1 axis induce sustained clinical responses in a sizable minority of cancer patients. We found that primary resistance to ICIs can be attributed abnormal gut microbiome composition. Antibiotics inhibited benefit patients with advanced cancer. Fecal microbiota transplantation (FMT) from who responded into germ-free or antibiotic-treated mice ameliorated antitumor effects PD-1 blockade, whereas FMT nonresponding failed do so....
Antibiotics (ABX) compromise the efficacy of programmed cell death protein 1 (PD-1) blockade in cancer patients, but mechanisms underlying their immunosuppressive effects remain unknown. By inducing down-regulation mucosal addressin adhesion molecule (MAdCAM-1) ileum, post-ABX gut recolonization by Enterocloster species drove emigration enterotropic α4β7+CD4+ regulatory T 17 cells into tumor. These deleterious ABX were mimicked oral gavage species, genetic deficiency, or antibody-mediated...
The clinical outcome of colorectal cancer (CRC) is associated with the immune response; thus, these tumors could be responsive to different therapy approaches. Natural killer (NK) cells are key antitumor primary effectors that can eliminate CRC without prior immunization. We previously determined NK from local tumor environment display a profoundly altered phenotype compared circulating healthy donors (HD). In this study, we evaluated peripheral blood untreated patients and their possible...
T cells that recognize tumor-specific mutations are crucial for cancer immunosurveillance and in adoptive transfer of TILs or transgenic-TCR cell products. However, their challenging identification isolation limits use clinical practice. Therefore, novel approaches to isolate needed. Here, we report the neoantigen-specific CD8+ from a vaccination site metastatic breast patient who received personalized vaccine. Based on somatic mutations, potential MHC binding epitopes were predicted, which...
Triple negative breast cancers (TNBC) lacking hormone receptors and HER-2 amplification are very aggressive tumors. Since relevant differences between primary tumors metastases could arise during tumor progression as evidenced by phenotypic discordances reported for hormonal or expression, in this analysis we studied changes that occurred our TNBC model IIB-BR-G throughout the development of IIB-BR-G-MTS6 metastasis to lymph nodes (LN) nude mice, using an antibody-based protein array...
Peptide-loaded MHC class I (pMHC-I) multimers have revolutionized our capabilities to monitor disease-associated T cell responses with high sensitivity and specificity. To improve the discovery of receptors (TCR) targeting neoantigens individual tumor patients recombinant molecules, we developed a peptide-loadable platform termed MediMer. MediMers are based on soluble disulfide-stabilized β 2 -microglobulin/heavy chain ectodomain single-chain dimers (dsSCD) that can be easily produced in...
Abstract Ileal epithelial cell apoptosis and the local microbiota modulate effects of oxaliplatin against proximal colon cancer by modulating tumor immunosurveillance. Here, we identified an ileal immune profile associated with prognosis responses to chemotherapy. The whole transcriptome was upregulated in poor-prognosis patients cancer, while colonic immunity healthy neoplastic areas downregulated (except for Th17 fingerprint) such patients. Similar observations were made across...
Abstract This article is part of the Dendritic Cell Guidelines series, which provides a collection state‐of‐the‐art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization mouse human dendritic cells (DC) from lymphoid organs various nonlymphoid tissues. DC are sentinels immune system present in almost every mammalian organ. Since they represent rare cell population, need to be extracted with that specifically...
Breast cancer (BC) is a highly heterogeneous disease presenting broad range of clinical and molecular characteristics. In the past years, growing body evidence demonstrated that immune response plays significant role in outcome. However, prognostic markers are not completely validated practice BC patients.With aim to characterize features, several parameters were analyzed peripheral blood at diagnosis 85 nonmetastatic patients between April 2011 July 2014.With median follow-up 38.6 months,...
Abstract Treatment with combined immune checkpoint blockade (CICB) targeting CTLA-4 and PD-1 is associated clinical benefit across tumor types, but a high rate of immune-related adverse events (irAE). Insights into biomarkers mechanisms response toxicity to CICB are needed. To address this, we profiled the blood, gut microbiome 77 advanced melanoma patients treated CICB, any ≥Grade 3 irAEs (49%) parallel studies in pre-clinical models. Tumor-associated genomic were similar those identified...
Why PD-1 blockade is ineffective in the vast majority of colorectal cancers (CCs) lacking microsatellite instability but harboring high densities tumor infiltrating T cells and follicular helper (TFH) B remained so far an open conundrum. In a recent report published Nature Medicine, we bring evidence mice patients that ileal microbiota turns tolerogenic apoptosis intestinal epithelial (IEC) into immunogenic cell demise capable eliciting IL-1β-dependent TFH responses benefit from anti-PD1 antibodies.