A5006781089- Immune cells in cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Immune responses and vaccinations
- RNA Research and Splicing
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Zebrafish Biomedical Research Applications
- Congenital gastrointestinal and neural anomalies
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Clinical Nutrition and Gastroenterology
- Infant Nutrition and Health
- Phagocytosis and Immune Regulation
University of Bonn
2022-2025
Quantitative BioSciences
2025
The lung is constantly exposed to the outside world and optimal adaptation of immune responses crucial for efficient pathogen clearance. However, mechanisms that lead lung-associated macrophages' functional developmental remain elusive. To reveal such mechanisms, we developed a reductionist model environmental intranasal β-glucan exposure, allowing detailed interrogation molecular pulmonary macrophage adaptation. Employing single-cell transcriptomics, high-dimensional imaging flow cytometric...
The transition of an embryo from gastrulation to organogenesis requires precisely coordinated changes in gene expression, but the underlying mechanisms remain unclear. RNA-binding protein Trim71 is essential for development and serves as a potent regulator post-transcriptional expression. Here, we show that global deficiency induces severe defects mesoderm-derived cells at onset organogenesis. Murine -KO embryos displayed impaired primitive erythropoiesis, yolk sac vasculature, heart...
Abstract This article is part of the Dendritic Cell Guidelines series, which provides a collection state‐of‐the‐art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization mouse human dendritic cells (DC) from lymphoid organs various nonlymphoid tissues. DC are sentinels immune system present in almost every mammalian organ. Since they represent rare cell population, need to be extracted with that specifically...
Abstract The transition of an embryo from gastrulation to organogenesis requires precisely coordinated changes in gene expression. RNA-binding protein Trim71 is essential for embryonic survival, but its exact role mammalian development vivo remains poorly defined. Here we show that murine -KO embryos appear normal until day (E)8.5 display severe defects primitive erythropoiesis, yolk sac vasculature and heart function during the onset at E9.5 E10.5. This led impaired vascular translocation...
Summary The lung is constantly exposed to the outside world and optimal adaptation of immune responses crucial for efficient pathogen clearance. However, mechanisms which lead functional developmental lung-associated macrophages remain elusive. To reveal such mechanisms, we developed a reductionist model environmental intranasal β-glucan exposure, allowing detailed interrogation molecular pulmonal macrophage adaptation. Employing single-cell transcriptomics, high dimensional imaging flow...