A5028772941- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Immune responses and vaccinations
- Drug Transport and Resistance Mechanisms
- Neonatal and fetal brain pathology
- Animal testing and alternatives
- Invertebrate Immune Response Mechanisms
- vaccines and immunoinformatics approaches
- Infant Development and Preterm Care
- Neural dynamics and brain function
- Metabolomics and Mass Spectrometry Studies
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Advanced Biosensing Techniques and Applications
- Cell Adhesion Molecules Research
- Infectious Diseases and Mycology
- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Phagocytosis and Immune Regulation
- Cancer Immunotherapy and Biomarkers
Johannes Gutenberg University Mainz
2015-2024
University Medical Center of the Johannes Gutenberg University Mainz
2015-2024
Sanquin
2013-2016
Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg-Universität Mainz
2015
Amsterdam UMC Location University of Amsterdam
2014
Amsterdam UMC Location Vrije Universiteit Amsterdam
2008-2010
The spleen is the lymphoid organ that induces immune responses toward blood-borne pathogens. Specialized macrophages in splenic marginal zone are strategically positioned to phagocytose pathogens and cell debris, but not known play a role activation of T-cell responses. Here we demonstrate metallophilic (MMM) essential for cross-presentation antigens by dendritic cells (DCs). Our data targeted MMM as well adenoviruses efficiently captured exclusively transferred CD8 + DCs cytotoxic T...
Multiple sclerosis is an inflammatory demyelinating disease of the central nervous system in which autoreactive myelin-specific T cells cause extensive tissue damage, resulting neurological deficits. In process, are primed periphery by antigen presenting dendritic (DCs). DCs considered to be crucial regulators specific immune responses and molecules or proteins that regulate DC function therefore under investigation. We here investigated potential immunomodulatory capacity ATP binding...
Generation of effective immune responses requires expansion rare antigen-specific CD4(+) T cells. The magnitude the responding population is ultimately determined by proliferation and survival. Both processes are tightly controlled to limit innocuous antigens. Sustained occurs only when innate sensors activated microbial stimuli or adjuvants, which has important implications for vaccination. molecular identity signals controlling sustained T-cell not fully clear. Here, we describe a...
Mouse splenic dendritic cell (DC) subsets possess distinct antigen-presentation abilities. CD8(+) DC are specialized in cross-presentation of antigens to T cells, whereas CD8(-) more efficient antigen presentation CD4(+) cells. In this study, we examined the capacity and present fungal MHC class I II molecules respectively. We used ovalbumin-expressing Saccharomyces cerevisiae (yeast-OVA) as a model system. Both phagocytosed yeast equal amounts uptake was mediated via dectin-1. addition,...
Abstract This article is part of the Dendritic Cell Guidelines series, which provides a collection state‐of‐the‐art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization mouse human dendritic cells (DC) from lymphoid organs various nonlymphoid tissues. DC are sentinels immune system present in almost every mammalian organ. Since they represent rare cell population, need to be extracted with that specifically...
This article is part of the Dendritic Cell Guidelines series, which provides a collection state-of-the-art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization mouse human DC from lymphoid organs, various non-lymphoid tissues. Within this chapter, detailed are presented that allow generation single-cell suspensions lymphohematopoietic tissues including spleen, peripheral lymph nodes, thymus, with focus on...
Abstract This article is part of the Dendritic Cell Guidelines series, which provides a collection state‐of‐the‐art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization mouse human dendritic cells (DC) from lymphoid organs various non‐lymphoid tissues. Recent studies have provided evidence an increasing number phenotypically distinct conventional DC (cDC) subsets that on one hand exhibit certain plasticity, but...
Significance Conventional dendritic cells (cDC) and macrophages display a high phenotypic functional heterogeneity. ADAM10 regulates diverse cellular activities via so-called ectodomain shedding of cell-surface proteins. Here, we report that mice with CD11c-specific deletion exhibit defective cDC subset homeostasis in the splenic marginal zone (MZ). Specifically, ESAM hi cDC2A are replaced by CX3CR1 + cDC2B population, terminal differentiation cDC1 is abrogated. Moreover, absent leads to...
Background: Multiple sclerosis is an inflammatory demyelinating disease of the central nervous system in which autoreactive myelin-specific T cells cause extensive tissue damage, resulting neurological deficits.In process, are primed periphery by antigen presenting dendritic (DCs).DCs considered to be crucial regulators specific immune responses and molecules or proteins that regulate DC function therefore under investigation.We here investigated potential immunomodulatory capacity ATP...
Dendritic cells (DCs) are present in almost all tissues, where they act as sentinels involved innate recognition and the initiation of adaptive immune responses. The DC family consists several cell lineages that heterogenous their development, phenotype, function. Within these lineages, further subdivisions exist, resulting smaller, less characterized subpopulations, each with its unique immunomodulatory capabilities. Given interest utilizing for experimental studies vaccination purposes, it...
Abstract Activated CD8+ T-cells must choose between different effector cell fates. Two major fates are the short lived terminal (TEC) fate and memory precursor (MPC) fate. Differentiation of immediately protective TECs is proportional to severity infection, suggesting existence signals that relay information about infection promote generation this type. We here show choice TEC MPC governed by inductive signaling via Notch. This surface receptor promotes differentiation correspondingly...