A5027561354- Neural Networks and Applications
- Radiation Therapy and Dosimetry
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Chronic Lymphocytic Leukemia Research
- DNA Repair Mechanisms
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Advanced Proteomics Techniques and Applications
- Epigenetics and DNA Methylation
- Endometrial and Cervical Cancer Treatments
- Advanced Breast Cancer Therapies
- Cervical Cancer and HPV Research
- Atherosclerosis and Cardiovascular Diseases
- T-cell and B-cell Immunology
- Vasculitis and related conditions
- Gene expression and cancer classification
- Metabolomics and Mass Spectrometry Studies
- Circular RNAs in diseases
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
- Lymphoma Diagnosis and Treatment
- Neural Networks and Reservoir Computing
- Microbial Metabolic Engineering and Bioproduction
University of Amsterdam
2016-2025
Amsterdam University Medical Centers
2018-2025
Public Health Service of Amsterdam
2023-2025
Amsterdam UMC Location University of Amsterdam
2013-2024
Institute of Infection and Immunity
2010-2024
Royal Netherlands Academy of Arts and Sciences
2024
Netherlands Bioinformatics Centre
2011-2019
Amsterdam Neuroscience
2019
Vrije Universiteit Amsterdam
2006-2019
Yonsei University
2016
Aberrant expression profiles of circulating microRNAs (miRNAs) have been described in various diseases and provide high sensitivity specificity. We explored miRNAs as potential biomarkers patients with heart failure (HF).The goal this study was to determine whether allow distinguish clinical HF not only from healthy controls but also non-HF forms dyspnea.A miRNA array performed on plasma 12 patients. From array, we selected 16 for a second 39 50 cases reports dyspnea, whom 30 were diagnosed...
Abstract The micronutrient zinc has an essential role in physiological and metabolic processes plants as a cofactor or structural element 300 catalytic noncatalytic proteins, but it is very toxic when available elevated amounts. Plants tightly regulate their internal concentrations process called homeostasis. exceptional hyperaccumulator species Thlaspi caerulescens can accumulate up to 3% of zinc, also high amounts nickel cadmium, without any sign toxicity. This should have drastic effects...
ABSTRACT Cadmium (Cd) is a widespread, naturally occurring element present in soil, rock, water, plants and animals. Cd non‐essential for toxic at higher concentrations. Transcript profiles of roots Arabidopsis thaliana ( ) Thlaspi caerulescens exposed to zinc (Zn) are examined, with the main aim determine differences gene expression between Cd‐tolerant Zn‐hyperaccumulator T. Cd‐sensitive non‐accumulator . This comparative transcriptional analysis emphasized role genes involved lignin,...
Perennial plants live for more than 1 year and flower only after an extended vegetative phase. We used Arabis alpina, a perennial relative of annual Arabidopsis thaliana, to study how increasing age exposure winter cold (vernalization) coordinate establish competence flower. show that the APETALA2 transcription factor, target microRNA miR172, prevents flowering before vernalization. Additionally, miR156 levels decline as A. alpina ages, causing increased production SPL (SQUAMOSA PROMOTER...
Disrupting the costimulatory CD40-CD40L dyad reduces atherosclerosis, but can result in immune suppression. The authors recently identified small molecule inhibitors that block interaction between CD40 and tumor necrosis factor receptor-associated (TRAF) 6 (TRAF-STOPs), while leaving CD40-TRAF2/3/5 interactions intact, thereby preserving CD40-mediated immunity.This study evaluates potential of TRAF-STOP treatment atherosclerosis.The effects TRAF-STOPs on atherosclerosis were investigated...
Abstract Microglia nodules (HLA-DR + cell clusters) are associated with brain pathology. In this post-mortem study, we investigated whether they represent the first stage of multiple sclerosis (MS) lesion formation. We show that microglia more severe MS Compared to in stroke, those enhanced expression genes previously found upregulated lesions. Furthermore, lipid metabolism, presence T and B cells, production immunoglobulins cytokines, activation complement cascade, metabolic stress MS....
CD8+ T cells play a critical role in the immune response to viral pathogens. Persistent human cytomegalovirus (HCMV) infection results strong increase number of virus-specific, quiescent effector-type with constitutive cytolytic activity, but molecular pathways involved induction and maintenance these are unknown. We show here that HCMV induced acute lasting changes transcriptomes virus-reactive collected from HCMV-seropositive patients at distinct stages infection. Enhanced cell cycle...
Presence of pelvic lymph node metastases is the main prognostic factor in early-stage cervical cancer patients, primarily treated with surgery. Aim this study was to identify cellular tumor pathways associated metastasis cancer.Gene expression profiles (Affymetrix U133 plus 2.0) 20 patients negative (N(0)) and 19 positive nodes (N(+)), were compared gene sets that represent all 285 presently available pathway signatures. Validation immunostaining tumors 274 consecutive performed for...
Slowing down translation in either the cytosol or mitochondria is a conserved longevity mechanism. Here, we found non-interventional natural correlation of mitochondrial and cytosolic ribosomal proteins (RPs) mouse population genetics, suggesting translational balance. Inhibiting C. elegans through mrps-5 RNAi repressed translation. Transcriptomics integrated with proteomics revealed that this inhibition specifically reduced efficiency mRNAs required growth pathways while increasing stress...
Human cytomegalovirus (CMV) induces the formation of effector CD8 + T cells that are maintained for decades during latent stage infection. Effector appear quiescent, but maintain constitutive cytolytic capacity and can immediately produce inflammatory cytokines such as IFN‐γ after stimulation. It is unclear how be constitutively in a terminal differentiation absence overt viral replication. We have recently described zinc finger protein Homolog Blimp‐1 (Hobit) murine NKT cells. Here, we show...
Animal studies have indicated that disturbed diurnal rhythms of clock gene expression in adipose tissue can induce obesity and type 2 diabetes. The importance the circadian timing system for energy metabolism is well established, but little known about regulation (clock) obese individuals with In this study we aimed to identify key disturbances white transcriptome diabetes.In a case-control design, included six diabetes healthy, lean control individuals. All participants were provided three...
Delineating the origins and properties of antibodies elicited by SARS-CoV-2 infection vaccination is critical for understanding their benefits potential shortcomings. Therefore, we investigate spike (S)-reactive B cell repertoire in unexposed individuals flow cytometry single-cell sequencing. We show that ∼82% S-reactive cells harbor a naive phenotype, which represents an unusually high fraction total human (∼0.1%). Approximately 10% these share IGHV1-69/IGKV3-11 receptor pairing, enrichment...
Background Coronary artery disease (CAD) is the leading cause of human morbidity and mortality worldwide, underscoring need to improve diagnostic strategies. Platelets play a major role, not only in process acute thrombosis during plaque rupture, but also formation atherosclerosis itself. MicroRNAs are endogenous small non-coding RNAs that control gene expression expressed tissue disease-specific manner. Therefore they have been proposed be useful biomarkers. It remains unknown whether...
Since the discovery of microRNAs (miRNAs), circulating miRNAs have been proposed as biomarkers for disease. Consequently, many groups tried to identify miRNA various types diseases including cardiovascular disease and cancer. However, replicability these experiments has disappointingly low. In order candidate biomarkers, in general, first an unbiased high-throughput screen is performed which a large number detected quantified circulation. Because are costly experiments, such studies using...
Circulating microRNAs (miRNAs) have been reported as biomarkers for disease diagnosis. RT-qPCR is most commonly used to detect miRNAs; however, no consensus on the appropriate method data normalization exists. Via a standardized selection method, we aimed determine separate miRNA panels measurements whole blood, platelets, and serum. Candidate miRNAs were selected from studies describing circulating microarray in Gene Expression Omnibus or ArrayExpress. expression of healthy controls...
Abstract Background Janus kinases (JAKs) mediate cytokine signaling involved in inflammatory bowel disease. The pan-JAK inhibitor tofacitinib has shown efficacy the treatment of ulcerative colitis. However, concerns regarding adverse events due to their wide spectrum inhibition fueled efforts develop selective JAK inhibitors. Given crucial role myeloid cells intestinal immune homeostasis, we evaluated effect and inhibitors on pro- anti-inflammatory macrophage polarization function (M1/M2)...
Obesity is associated with low-grade inflammation and insulin resistance (IR). The contribution of adipose tissue (AT) hepatic to IR remains unclear. We conducted a study across three cohorts investigate this relationship.The first cohort consists six women normal weight twenty obesity. In obesity, we found an upregulation inflammatory markers in subcutaneous visceral tissue, isolated AT macrophages, the liver, but no linear correlation tissue-specific sensitivity. second cohort, studied 24...