A5049322687- Multiple Sclerosis Research Studies
- Vitamin D Research Studies
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Psoriasis: Treatment and Pathogenesis
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- Whipple's Disease and Interleukins
- Immunotherapy and Immune Responses
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Systemic Lupus Erythematosus Research
- Peripheral Neuropathies and Disorders
- Polyomavirus and related diseases
- Microscopic Colitis
- Stress Responses and Cortisol
- Autoimmune and Inflammatory Disorders Research
- Tryptophan and brain disorders
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Vitamin C and Antioxidants Research
- Systemic Sclerosis and Related Diseases
- Immune cells in cancer
- Neurogenesis and neuroplasticity mechanisms
- Health Systems, Economic Evaluations, Quality of Life
- Rheumatoid Arthritis Research and Therapies
Netherlands Institute for Neuroscience
2013-2025
Erasmus MC
2020-2025
University Medical Center
2010-2024
Coöperatie VGZ (Netherlands)
2024
Erasmus University Rotterdam
2020-2023
Amsterdam Neuroscience
2020-2022
Amsterdam University Medical Centers
2021-2022
University of Amsterdam
2021-2022
Royal Netherlands Academy of Arts and Sciences
2013-2022
Huygens Institute for History and Culture of the Netherlands
2021
Multiple Sclerosis is associated with low serum levels of 25-hydroxyvitamin D (25(OH)D). We investigated the association between 25(OH)D and 1,25-dihydroxyvitamin (1,25(OH)2D), biologically active metabolite, clinical MS severity as expressed by EDSS-score relapse rate.Cross-sectional study.Serum samples from 267 patients were collected for 1,25(OH)2D measurement. Clinical parameters at date sampling determined.Both metabolite significantly lower in progressive forms compared to relapsing...
Abstract Most tissues are populated by tissue-resident memory T cells (T RM cells), which adapted to their niche and appear be indispensable for local protection against pathogens. Here we show that human white matter-derived brain CD8 + can subsetted into CD103 − CD69 both with a phenotypic transcription factor profile consistent cells. Specifically, expression in correlates reduced of differentiation markers, increased tissue-homing chemokine receptors, intermediate low the factors T-bet...
In several autoimmune diseases, including multiple sclerosis (MS), a compromised regulatory T cell (Treg) function is believed to be critically involved in the disease process. vitro, biologically active metabolite of vitamin D has been shown promote Treg development. A poor status linked with MS incidence and activity. present study, we assess potential vivo correlation between relapsing remitting (RRMS) patients.Serum levels 25-hydroxyvitamin (25(OH)D) were measured 29 RRMS patients. The...
Abstract Multiple sclerosis is a chronic inflammatory, demyelinating disease, although it has been suggested that in the progressive late phase, inflammatory lesion activity declines. We recently showed Netherlands Brain Bank multiple sclerosis-autopsy cohort considerable ongoing also at end stage of based on microglia/macrophage activity. have now studied role T cells this autopsy cases. quantified and perivascular T-cell cuffing standardized location medulla oblongata 146 sclerosis, 20...
Abstract Microglia nodules (HLA-DR + cell clusters) are associated with brain pathology. In this post-mortem study, we investigated whether they represent the first stage of multiple sclerosis (MS) lesion formation. We show that microglia more severe MS Compared to in stroke, those enhanced expression genes previously found upregulated lesions. Furthermore, lipid metabolism, presence T and B cells, production immunoglobulins cytokines, activation complement cascade, metabolic stress MS....
A poor vitamin D status has been associated with a high disease activity of multiple sclerosis (MS). Recently, we described associations between and peripheral T cell characteristics in relapsing remitting MS (RRMS) patients. In the present study, studied effects dose D3 supplementation on safety related outcome measures.Fifteen RRMS patients were supplemented 20,000 IU/d for 12 weeks. Vitamin calcium metabolism carefully monitored, by flowcytometry. All finished protocol without...
Abstract Much is still unknown about mechanisms underlying the phenotypical and functional versatility of human microglia. Therefore, we developed a rapid procedure to isolate pure microglia from postmortem brain tissue studied their immediate ex vivo phenotype responses key inflammatory mediators. Microglia were isolated, along with macrophages choroid plexus by dissociation, density gradient separation, selection magnetic microbeads. By flow cytometry, identified CD11b + CD45 dim smaller...
Multiple sclerosis (MS) is an inflammatory and neurodegenerative demyelinating disease of the central nervous system (CNS), most likely autoimmune in origin, usually beginning early adulthood. The aetiology not well understood; it viewed currently as a multifactorial which results from complex interactions between genetic predisposition environmental factors, few are potentially modifiable. Improving our understanding these factors can lead to new more effective approaches patient...
Vitamin D deficiency has been implicated as a risk factor for multiple sclerosis (MS), but how vitamin metabolism affects MS pathophysiology is not understood. We studied the expression of receptor (VDR) and related enzymes, including 1,25(OH)2D-24-hydroxylase (24-OHase; CYP24A1) 25(OH)D-1α-hydroxylase (CYP27B1), in CNS tissues 39 patients 20 controls primary human glial cells vitro. In control normal-appearing white matter (NAWM), nuclear VDR immunostaining was observed oligodendrocyte-like...
<h3>Objective</h3> In the phase II, randomized, double-blind, placebo-controlled Supplementation of Vigantol Oil versus Placebo Add-on in Patients with Relapsing-Remitting Multiple Sclerosis (RRMS) Receiving Rebif Treatment (SOLAR) study (NCT01285401), we assessed efficacy and safety add-on vitamin D<sub>3</sub> patients RRMS. <h3>Methods</h3> Eligible RRMS treated SC interferon-β-1a (IFN-β-1a) 44 μg 3 times weekly serum 25(OH)D levels <150 nmol/L were included. From February 15, 2011, to...
Microglia are key players in the central nervous system health and disease. Much pioneering research on microglia function has been carried out vivo with use of genetic animal models. However, to fully understand role neurological psychiatric disorders, it is crucial study primary human from brain donors. We have developed a rapid procedure for isolation pure autopsy tissue using density gradient centrifugation followed by CD11b-specific cell selection. The protocol can be completed 4 h, an...
<h3>Background and Objectives</h3> To investigate whether white matter lesion activity, acute axonal damage, density in MS associate with CSF neurofilament light chain (NfL) levels. <h3>Methods</h3> Of 101 brain donors (n = 92 progressive MS, n 9 relapsing-remitting MS), ventricular was collected, NfL levels were measured. White lesions classified as active, mixed, inactive, or remyelinated, microglia/macrophage morphology active mixed ramified, ameboid, foamy. In addition, damage assessed...
Abstract Multiple sclerosis (MS) is a heterogeneous neurological disorder with regards to clinical presentation and pathophysiology. Here, we investigated the heterogeneity of MS by performing an exploratory factor analysis on quantitative qualitative neuropathology data collected for 226 donors in Netherlands Brain Bank autopsy cohort. Three promising dimensions were identified subsequently validated clinical, neuropathological, genetic data. Dimension 1 ranged from predominance...
Introduction Remyelination of demyelinated axons can occur as an endogenous repair mechanism in multiple sclerosis (MS), but its efficacy varies between both MS individuals and lesions. The molecular cellular mechanisms that drive remyelination remain poorly understood. Here, we studied the relation microglia activation activity MS. Methods We correlated regenerative (CD163 + ) inflammatory (iNOS with BCAS1 oligodendrocytes, subdivided into early-stage (&lt;3 processes) late-stage (≥3...
ABSTRACT Background Relapsing–remitting (RR) and primary progressive (PP) multiple sclerosis (MS) have distinct clinical courses, but underlying pathophysiological differences remain unclear. We compared pathological components between RRMS, PPMS, other inflammatory neurodegenerative disorders, leveraging soluble biomarkers post‐mortem pathology. Methods Serum cerebrospinal fluid (CSF) of people diagnosed with (pw) PPMS ( n = 104), RRMS 38), Alzheimer's disease (AD, 22), neuromyelitis optica...