A5040159978- Immune Cell Function and Interaction
- Immune cells in cancer
- Animal testing and alternatives
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolomics and Mass Spectrometry Studies
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- T-cell and B-cell Immunology
University of Bonn
2020-2022
Significance Obesity-associated metaflammation is an emerging disease, in which proinflammatory macrophages play a decisive role. Here, we identified the soluble mannose receptor (sMR) as regulator of and demonstrated increased levels sMR obese mice humans. Additionally, that directly binds to CD45 on macrophages, inhibiting its activity inducing inflammatory macrophage activation via Akt/NF-κB pathway. Consequently, induced metabolic tissue aggravation whole-body homeostasis. These data...
Abstract This article is part of the Dendritic Cell Guidelines series, which provides a collection state‐of‐the‐art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization mouse human dendritic cells (DC) from lymphoid organs various non‐lymphoid tissues. Recent studies have provided evidence an increasing number phenotypically distinct conventional DC (cDC) subsets that on one hand exhibit certain plasticity, but...
Abstract Pro-inflammatory activation of macrophages in metabolic tissues is critically important induction obesity-induced metaflammation. Here, we demonstrate that the soluble mannose receptor (sMR) plays a direct, functional role both macrophage and We show sMR binds CD45 on inhibits its phosphatase activity, leading to Src/Akt/NF-κB-mediated cellular reprogramming towards an inflammatory phenotype vitro vivo. Remarkably, increased serum levels were observed obese mice humans directly...