A5103149432- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- IL-33, ST2, and ILC Pathways
- Colorectal and Anal Carcinomas
- Polyamine Metabolism and Applications
- Hepatitis B Virus Studies
- Phagocytosis and Immune Regulation
- Diabetes and associated disorders
- Chemokine receptors and signaling
- Trace Elements in Health
- Hepatitis C virus research
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Cytomegalovirus and herpesvirus research
- Software Engineering Techniques and Practices
- Cell Adhesion Molecules Research
- Pancreatic and Hepatic Oncology Research
- PI3K/AKT/mTOR signaling in cancer
- HER2/EGFR in Cancer Research
- Liver Disease Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
Centre International de Recherche en Infectiologie
2020-2024
Inserm
2020-2024
Université Claude Bernard Lyon 1
2020-2024
Centre National de la Recherche Scientifique
2020-2024
University of Würzburg
2024
École Normale Supérieure de Lyon
2020-2024
Centre de Recherche en Cancérologie de Lyon
2021-2023
Centre Léon Bérard
2022-2023
Fundación Instituto Leloir
2012-2017
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2017
Despite NK cells being originally identified because of their ability to kill tumor in vitro, only limited information is available on infiltration malignant tumors, especially humans. infiltrating human colorectal carcinomas (CRCs) were analyzed identify potential protective role an antitumor immune response. The expression and function relevant molecules from different sources, comparing tumor-associated (TANKs) with autologous peripheral blood (PB-NKs) CRC patients-the latter comparison...
The clinical outcome of colorectal cancer (CRC) is associated with the immune response; thus, these tumors could be responsive to different therapy approaches. Natural killer (NK) cells are key antitumor primary effectors that can eliminate CRC without prior immunization. We previously determined NK from local tumor environment display a profoundly altered phenotype compared circulating healthy donors (HD). In this study, we evaluated peripheral blood untreated patients and their possible...
Antiviral effectors such as natural killer (NK) cells have impaired functions in chronic hepatitis B (CHB) patients. The molecular mechanism responsible for this dysfunction remains poorly characterised. We show that decreased cytokine production capacity of peripheral NK from CHB patients was associated with reduced expression NKp30 and CD16, defective mTOR pathway activity. Transcriptome analysis revealed an enrichment transcripts expressed exhausted T suggesting cell exhaustion employ...
Objective The hallmark oncogene MYC drives the progression of most tumours, but direct inhibition by a small-molecule drug has not reached clinical testing. is transcription factor that depends on several binding partners to function. We therefore explored possibility targeting via its interactome in pancreatic ductal adenocarcinoma (PDAC). Design To identify suitable targets among all partners, we constructed targeted shRNA library and performed screens cultured PDAC cells tumours mice....
Clinical studies suggest that triple negative breast cancer (TNBC) patients with epidermal growth factor receptor (EGFR)‐expressing tumors could benefit from therapy Cetuximab, which targets EGFR. NK cells are the primary effectors of antibody (Ab)‐dependent cell‐mediated cytotoxicity (ADCC) and thus play a role in Ab‐based therapies. We have previously described diminished levels Cetuximab‐mediated ADCC vitro advanced cancer. Here, we investigated potential causes this NK‐cell functional...
Natural killer (NK) cells often become dysfunctional during tumor progression, but the molecular mechanisms underlying this phenotype remain unclear. To explore phenomenon, we set up mouse lymphoma models activating or not NK cells. Both types elicited type I interferon production, leading to expression of a T cell exhaustion-like signature in cells, which included immune checkpoint proteins (ICPs). However, dysfunction occurred exclusively model that triggered activation. Moreover,...
NK cells are cytotoxic lymphocytes displaying strong antimetastatic activity. Mouse models and in vitro studies suggest a prominent role of the mechanistic target rapamycin (mTOR) kinase control cell homeostasis antitumor functions. However, mTOR inhibitors used as chemotherapies several cancer settings. The impact such treatments on patients' is unknown. We thus performed immunophenotyping circulating from metastatic breast patients treated with inhibitor everolimus over three-month period....
NK cell receptors allow cells to recognize targets such as tumor cells. Many of them are expressed on a subset cells, independently each other, which creates vast diversity receptor combinations. Whether these combinations influence antitumor responses is not well understood. We addressed this question in the C57BL/6 mouse model and analyzed individual effector response 444 subsets, defined by 12 receptors, against lines originating from different tissues strains. found wide range reactivity...
Abstract Natural Killer (NK) cell subsets differ to ensure complementary and crucial roles in tumor immunosurveillance. Their biology is critically regulated by cytokines. Here, we show that IL-33 synergizes with IL-12 strongly activate a subset of CD56 dim NK cells acquiring ST2 expression. Transcriptomic biological analysis human + revealed distinct intermediate differentiation state between canonical bright cells, combining high proliferative properties, cytokines/chemokines production,...
Abstract A significant proportion of individuals infected by HBV develops chronic infection. Antiviral effectors such as Natural Killer (NK) cells have impaired functions in these patients, but the molecular mechanism responsible for this dysfunction remains poorly characterized. Here, we show that peripheral NK from hepatitis B (CHB) patients a defective capacity to produce IFN-γ, MIP1-β and TNF-α retain an intact killing capacity. This functional phenotype was associated with decrease...
Abstract Background and Objective: Approximately 10 – 15% of Breast Cancers (BC) are known to be ‘triple-negative (TN) receptor’, not expressing Estrogen receptor, Progesterone receptor exhibiting overexpression and/or gene amplification HER2-neu. TNBC is associated with a more aggressive clinical course worse outcome than non-TNBC. As patients likely benefit from anti-estrogen or anti-HER2 therapy, first-line treatment usually consists conventional cytotoxic chemotherapy. Taking into...
Abstract Background: While the majority of Triple Negative Breast Cancer (TNBC) overexpress epidermal growth factor receptor (EGFR), EGFR inhibition as a monotherapy with Cetuximab is not effective. The study its mechanism action important to find out potentially synergistic combinations. Previously, we demonstrated that can trigger antibody-dependent NK cell-mediated cytotoxicity against TNBC cells. aim this was investigate effect cell activation by Cetuximab-coated cells on Dendritic Cell...
Abstract Background: In a Phase II/III clinical trial (CASVAC-0401, NCT01729663), cutaneous melanoma (CM) patients (pts) stages were randomized to receive CSF-470 vaccine or IFN-α (2:1). Vaccine is composed of four lethally-irradiated allogeneic CM cell lines; BCG (Bacillus Calmette-Guérin) and rhGM-CSF used as adjuvants. At the end II part study (30 pts), higher distant-metastases-free-survival was observed in vaccinated pts. All pts developed inflammation at vaccination site. Therefore, an...
Natural Killer (NK) cell receptors allow NK cells to recognize targets such as tumor cells. Many of them are expressed on a subset cells, independently each other, which creates vast diversity receptor combinations. Whether these combinations influence anti-tumor responses is not well understood. We addressed this question in the C57BL/6 mouse model and analyzed individual effector response 444 subsets, defined by 12 receptors, against lines different tissue origins. found wide range...