A5079620095- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- Tryptophan and brain disorders
- Cancer, Stress, Anesthesia, and Immune Response
- Chemokine receptors and signaling
- Monoclonal and Polyclonal Antibodies Research
- Cancer Mechanisms and Therapy
- Psoriasis: Treatment and Pathogenesis
- Immune cells in cancer
- Lung Cancer Treatments and Mutations
- Cytokine Signaling Pathways and Interactions
- Ovarian cancer diagnosis and treatment
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Lymphoma Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Multiple Myeloma Research and Treatments
- IL-33, ST2, and ILC Pathways
- Angiogenesis and VEGF in Cancer
Institut universitaire du cancer de Toulouse Oncopole
2018-2025
Inserm
2014-2025
Centre de Recherche en Cancérologie de Toulouse
2018-2025
Université Toulouse III - Paul Sabatier
2018-2025
Centre National de la Recherche Scientifique
1997-2025
Fondation ARC pour la Recherche sur le Cancer
2025
Université de Toulouse
2021-2024
Institut Claudius Regaud
2019-2024
Institut de Cancérologie de l'Ouest
2012-2023
Institut Gustave Roussy
2016-2017
Phages and cancer immunity Gut bacteria are involved in the education of T cell immune responses, intestinal ecosystem influences anticancer immunity. Fluckiger et al. report microbial antigens that might cross-react with associated tumor cells. They found a type called enterococci harbor bacteriophage modulates responses. In mouse models, administration containing boosted responses after treatment chemotherapy or programmed death protein 1 (PD-1) blockade. humans, presence was improved...
Recent studies have suggested a close relationship between CD4(+)FOXP3(+) regulatory T cells (Tregs) and proinflammatory IL-17-producing helper (T(H)17) expressing the lineage-specific transcription factor RORgamma t. We report here unexpected finding that human memory Tregs secrete IL-17 ex vivo constitutively express IL-17-secreting share some phenotypic functional features with conventional T(H)17 cells, high levels of CCR4 CCR6 low CXCR3. However, unlike they CD161 mostly fail to...
Immune checkpoint blockers (ICB) have become pivotal therapies in the clinical armamentarium against metastatic melanoma (MMel). Given frequency of immune related adverse events and increasing use ICB, predictors response to CTLA-4 and/or PD-1 blockade represent unmet needs. Using a systems biology-based approach an assessment 779 paired blood tumor markers 37 stage III MMel patients, we analyzed association between parameters functional reactivity tumor-infiltrating cells after ex vivo...
CD4+CD25+ Tregs play a central role in the maintenance of peripheral self tolerance by keeping autoreactive T cells check. Whereas thymic origin Tregs, as distinct lineage, has been inferred, understanding their developmental pathways remained elusive. In both mice and humans, Treg populations have described composed antigen-experienced that fail to significantly proliferate following TCR stimulation but suppress proliferation effector functions CD25– cells. Here we show analysis CD25...
The use of recombinant tumor antigen proteins is a realistic approach for the development generic cancer vaccines, but potential this type vaccines to induce specific CD8(+) T cell responses, through in vivo cross-priming, has remained unclear. In article, we report that repeated vaccination patients with NY-ESO-1 protein, Montanide ISA-51, and CpG ODN 7909, potent stimulator B cells helper 1 (Th1)-type immunity, resulted early induction integrated CD4(+) Th antibody responses most...
NY-ESO-1 is a cancer/testis antigen expressed in range of human malignancies, and number vaccine strategies targeting are being developed. In the present study, safety immunogenicity recombinant vaccinia-NY-ESO-1 fowlpox-NY-ESO-1 were analyzed series 36 patients with different tumor types. Each construct was first tested individually at two dose levels then prime-boost setting followed by fowlpox-NY-ESO-1. The vaccines well tolerated either or together. NY-ESO-1-specific antibody responses...
Rapamycin is an immunosuppressive drug currently used in different clinical settings. Although the capacity of rapamycin to inhibit mammalian target serine/threonine protein kinase and therefore T cell cycle progression well known, its effects are complex not completely understood. It has been reported recently that TCR-mediated stimulation murine CD4+ cells presence results increased proportions with suppressive functions, suggesting may also exert activity by promoting selective expansion...
RORγt + T H 17 cells are a proinflammatory CD4 T-cell population associated with autoimmune tissue injury. In mice, priming of requires TGF-β, which alone directs the FOXP3 regulatory (Treg), in association inflammatory cytokines. Priming human from conventional naive under similar conditions, however, has proved difficult to achieve. Here, we report that differentiation preferentially occurs Treg (NTreg) presence IL-2 and IL-1β is increased by IL-23 TGF-β. IL-1β–mediated correlated IL-1RI...
Abstract It has been reported that levo-1-methyl tryptophan (L-1MT) can block indoleamine-2,3-dioxygenase (IDO) expressed by human dendritic cells (DC), whereas dextro-1-methyl (D-1MT) is inefficient. However, whether L-1MT or D-1MT efficiently reverse IDO-induced arrest of T-cell proliferation not clarified. Here, we show a marked immunosuppressive effect IDO derived from INDO-transfected 293 cell, IDO+ ovarian cancer cells, and monocyte-derived DCs on CD4+ Th1 CD8+ T natural killer...
Abstract Antitumor type I T-cell responses involving IFN-γ production are critical to control cancer, but the efficacy of this response is limited by a variety immunosuppressive mechanisms that promote tumoral immune escape. One mechanism involves accumulation FOXP3+ T regulatory cells (Treg), class suppressive prevent excessive tissue destruction caused unchecked responses. Recent studies have revealed Treg include distinct subsets specifically controlling over corresponding effector...
Whereas preclinical investigations and clinical studies have established that CD8+ T cells can profoundly affect cancer progression, the underlying mechanisms are still elusive. Challenging prevalent view beneficial effect of in is solely attributable to their cytotoxic activity, several reports indicated ability promote tumor regression dependent on cytokine secretion profile self-renew. Evidence has also shown microenvironment disarm cell immunity, leading emergence dysfunctional cells....
Tumor antigen–specific CD4 T cells accumulate at tumor sites, evoking their involvement in antitumor effector functions situ. Contrary to CD8 cytotoxic lymphocyte exhaustion, that of remains poorly appreciated. Here, using phenotypic, transcriptomic, and functional approaches, we characterized cell exhaustion patients with head neck, cervical, ovarian cancer. We identified a tumor-infiltrating (TIL) population, defined by high PD-1 CD39 expression, which contained proportions...
TNF blockers can be used to manage gastrointestinal inflammatory side effects following nivolumab and/or ipilimumab treatment in patients with advanced melanoma. Our preclinical data showed that anti-TNF could promote the efficacy of immune checkpoint inhibitors.TICIMEL (NTC03293784) is an open-label, two-arm phase Ib clinical trial. Fourteen metastatic melanoma (stage IIIc/IV) were enrolled. Patients treated (1 mg/kg) and (3 combined infliximab (5 mg/kg, N = 6) or certolizumab (400/200 mg,...
Abstract The tumor Ag SSX-2 (HOM-MEL-40) was found by serological identification of Ags recombinant expression cloning and shown to be a cancer/testis expressed in wide variety tumors. It may therefore represent source CD8+ T cell epitopes useful for specific immunotherapy cancer. To identify potential SSX-2-derived that can recognized cells, we used an approach combined: 1) the vitro proteasomal digestion precursor peptides overlapping complete sequence; 2) prediction with appropriate...
Abstract FOXP3+ regulatory T cells (Tregs) are critical regulators of self-tolerance and immune homeostasis. In mice humans, two subsets Tregs have been defined based on their differential expression Helios, a transcription factor the Ikaros family. Whereas origin, specificity, function as yet source controversy, characterization thus far has limited by absence surface markers to distinguish them. this article, we show that human memory Helios+ Helios− phenotypically distinct can be...
Despite the clinical success of blocking inhibitory immune checkpoint receptors such as programmed cell death-1 (PD-1) in cancer, mechanisms controlling expression these have not been fully elucidated. Here, we identify a post-transcriptional mechanism regulating PD-1 T cells. Upon activation, PDCD1 mRNA and ribonucleoprotein complexes coalesce into stress granules that require microtubules kinesin 1 molecular motor to proceed translation. Hence, is highly sensitive microtubule or granule...
Abstract The membrane receptor 2B4 is a CD2 family member that involved in lymphocyte activation. A fraction of human CD8+ αβ T cells up-regulate vivo, and here we demonstrate this correlates with the acquisition effector cell properties such as granzyme B perforin expression, rapid IFN-γ production, down-regulation lymph node homing chemokine CCR7. In PBLs from healthy donors, cytomegalovirus-specific were positive, whereas naive melanoma Ag (Melan-A/melanoma recognized by cells-1)-specific...