A5055279293- Extracellular vesicles in disease
- Inflammasome and immune disorders
- Cardiovascular Disease and Adiposity
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- MicroRNA in disease regulation
- Antioxidant Activity and Oxidative Stress
- Paraoxonase enzyme and polymorphisms
- Blood disorders and treatments
- Adipose Tissue and Metabolism
- Venous Thromboembolism Diagnosis and Management
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Heme Oxygenase-1 and Carbon Monoxide
- Systemic Lupus Erythematosus Research
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Inflammatory Myopathies and Dermatomyositis
- Diabetes and associated disorders
- Blood Coagulation and Thrombosis Mechanisms
- Infectious Aortic and Vascular Conditions
- Cynara cardunculus studies
- Aortic aneurysm repair treatments
- Protein Kinase Regulation and GTPase Signaling
- Phagocytosis and Immune Regulation
Philadelphia VA Medical Center
2016-2024
University of Pennsylvania
2015-2024
Second Military Medical University
2022
United States Department of Veterans Affairs
2019
Temple University
2010-2018
Veterans Health Administration
2018
Jagiellonian University
2014
University Medical Center
2014
Pohang University of Science and Technology
2014
AGH University of Krakow
2014
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
Objective— To investigate whether exposure of human monocytes/macrophages to tobacco smoke induces their release membrane microvesicles (MVs) that carry tissue factor (TF) released from cells, smoke-induced MVs are procoagulant, and what cellular processes might be responsible for production. Methods Results— We found THP-1 monocytes primary monocyte–derived macrophages 3.75% extract (TSE) significantly increased total TF-positive MV generation. More importantly, TSE-treated exhibited 3 4...
Cigarette smoking damages the extracellular matrix in a variety of locations, leading to atherosclerotic plaque instability and emphysematous lung destruction, but underlying mechanisms remain poorly understood. Here, we sought determine whether exposure human macrophages, key participant damage, tobacco smoke extract (TSE) induces release microvesicles (MVs; or microparticles) with proteolytic activity; major proteases involved; cellular that might mediate their generation. We found MVs...
OBJECTIVE: To search for determinants of endothelial dysfunction in type 2 diabetes. RESEARCH DESIGN AND METHODS: We performed a comprehensive analysis cardiovascular risk markers and measured blood flow responses to endothelium-dependent (acetylcholine [ACh] NG-monomethyl-L-arginine) -independent (sodium nitroprusside [SNP]) vasoactive agents 30 nonsmoking men with diabetes (age 51 +/- 1 years, BMI 27.8 0.4 kg/m2, HbA1c 7.4 0.3%) 12 matched normal control men. RESULTS: ACh-induced...
Physical activity increases the production of oxygen free radicals, which may consume antioxidants and oxidize low-density lipoprotein (LDL). To determine whether this occurs during strenuous aerobic exercise, we studied 11 well-trained runners who participated in Helsinki City Marathon. Blood samples were collected before, immediately after, 4 days after race to its effect on circulating LDL oxidizability vitro. was increased as determined from a reduction lag time for formation conjugated...
Biologically significant amounts of two procoagulant molecules, phosphatidylserine (PS) and tissue factor (TF), are transported by monocyte/macrophage-derived microvesicles (MVs). Because cellular cholesterol accumulation is an important feature atherosclerotic vascular disease, we now examined effects enrichment on MV release from human monocytes macrophages.Cholesterol THP-1 monocytes, alone or in combination with lipopolysaccharide (LPS), tripled their total generation, as quantified flow...
Objective— Cholesterol enrichment occurs in vivo when phagocytes ingest retained and aggregated lipoproteins, damaged or senescent cells, related debris. We previously reported that of human monocyte/macrophages with unesterified cholesterol (UC) triggers the release highly procoagulant microvesicles ([MVs], also called microparticles) through induction apoptosis. determined whether UC-induced MVs (UCMVs) might transmit endogenous danger signals and, if so, what molecular processes be...
Summary The intraluminal thrombus (ILT) of human abdominal aortic aneurysm (AAA) has been suggested to damage the underlying wall, but previous work found scant activity soluble proteases in abluminal layer ILT, adjacent aneurysm. We hypothesised that transmembrane carried by membrane microvesicles (MV) from dying cells remain active ILT. ILTs and AAA segments collected 21 patients during surgical repair were assayed for two major proteases, ADAM10 (a disintegrin metalloprotease-10) ADAM17....
Vascular endothelial dysfunction is the closely related determinant of ischemic heart disease (IHD). Endothelial and ischemia/reperfusion injury (IRI) have been associated with an increase in microvesicles (MVs) vivo. However, potential contribution (EMVs) to myocardial damage unclear. Here we aimed investigate role EMVs derived from hypoxia/reoxygenation (H/R) -treated human umbilical vein cells (HUVECs) on cultured H9c2 cardiomyocytes. H/R model was established induce HUVECs release...
High mobility group box 1 (HMGB1) is a nuclear protein that can be released from activated or dead cells. Extracellular HMGB1 serve as "danger signal" and novel cytokine mediates sterile inflammation. In addition to its soluble form, extracellular also carried by membrane microvesicles. However, the cellular mechanisms responsible for translocation plasma release onto microvesicles have not been investigated. Tobacco smoking major cause of inflammation in many diseases. Smoking increases...
Oxidized low-density lipoprotein (Ox-LDL)is implicated in the pathogenesis of atherosclerosis. Circulating oxidation-specific epitopes on plasma Ox-LDL has been linked with coronary artery disease, but its determinants and association early development atherosclerosis familial combined hyperlipidemia (FCHL) not very well studied. This study aimed to investigate circulating between carotid intima-media thickness (IMT) asymptomatic members FCHL families.Ox-LDL, susceptibility LDL oxidation...
Type 2 diabetes mellitus (T2DM) impairs hepatic clearance of atherogenic postprandial remnant lipoproteins. Our work and that others have identified syndecan-1 heparan sulfate proteoglycans (HSPGs) as lipoprotein receptors. Nevertheless, defects in the T2DM liver not been molecularly characterized, neither has correction occurs upon caloric restriction. We used microarrays to compare expression proteoglycan-related genes livers from control db/m mice; obese, db/db littermates fed ad libitum...
Objective— This study aims to determine whether and how the enriched metabolites of endothelial extracellular vesicles (eEVs) are critical for cigarette smoke-induced direct injury cells development pulmonary hypertension, rarely explored in contrast long-investigated mechanisms secondary chronic hypoxemia. Approach Results— Metabonomic screen eEVs from cigarette-smoking human subjects reveals prominent elevation spermine—a polyamine metabolite with potent agonist activity CaSR...