A5101786146- Pulmonary Hypertension Research and Treatments
- Salmonella and Campylobacter epidemiology
- Nitric Oxide and Endothelin Effects
- Vibrio bacteria research studies
- Ion Channels and Receptors
- Mitochondrial Function and Pathology
- Protein Kinase Regulation and GTPase Signaling
- Eicosanoids and Hypertension Pharmacology
- Listeria monocytogenes in Food Safety
- Aquaculture disease management and microbiota
- Viral gastroenteritis research and epidemiology
- ATP Synthase and ATPases Research
- Antibiotic Resistance in Bacteria
- Ion Transport and Channel Regulation
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Identification and Quantification in Food
- Ion channel regulation and function
- Extracellular vesicles in disease
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Renin-Angiotensin System Studies
- MicroRNA in disease regulation
- Venous Thromboembolism Diagnosis and Management
- Plant Stress Responses and Tolerance
- Peptidase Inhibition and Analysis
Huazhong University of Science and Technology
2016-2025
Xiangya Hospital Central South University
2020-2025
Central South University
2020-2025
Shenzhen Center for Disease Control and Prevention
2010-2024
Nanchang University
2024
Nanjing Forestry University
2024
Tongji Hospital
2017-2024
Wuhan University
2024
Renmin Hospital of Wuhan University
2024
Shanxi Medical University
2022-2023
Whether the growth hormone (GH)/insulin-like factor 1(IGF-1) axis exerts cardioprotective effects remains controversial; and underlying mechanism(s) for such actions are unclear. Here we tested hypothesis that hormone-releasing (GHRH) directly activates cellular reparative mechanisms within injured heart, in a GH/IGF-1 independent fashion. After experimental myocardial infarction (MI), rats were randomly assigned to receive, during 4-week period, either placebo (n = 14), rat recombinant GH...
Airway eosinophilic inflammation is a key feature of type 2 high asthma. The role epithelial microRNA (miR) in airway remains unclear. We examined the expression miR-221-3p bronchial brushings, induced sputum, and plasma from 77 symptomatic, recently diagnosed, steroid-naive subjects with asthma 36 healthy controls by quantitative PCR analyzed correlation between eosinophilia. found that epithelial, was significantly decreased Epithelial correlated eosinophil sputum biopsies, fraction...
Monocrotaline has been widely used to establish an animal model of pulmonary hypertension. The molecular target underlying monocrotaline-induced artery endothelial injury and hypertension remains unknown. extracellular calcium-sensing receptor (CaSR) particularly its domain hold the potential structural basis for monocrotaline bind. This study aimed reveal whether induces by targeting CaSR.Nuclear magnetic resonance screening through WaterLOGSY (water ligand-observed gradient spectroscopy)...
Mitochondria are essential for the onset of hypoxia-induced pulmonary vasoconstriction and vascular-remodeling, two major aspects underlying development hypertension, an incurable disease. However, hypoxia induces relaxation systemic arteries such as femoral mitochondrial heterogeneity controls distinct responses versus artery smooth muscle cells to in vitro. The aim this study was determine whether can be experimentally exploited vivo a potential treatment against hypertension. intact...
Metabolic reprogramming is a hallmark of pulmonary arterial hypertension. Platelet activation has been implicated in hypertension (PAH), whereas the role platelet pathogenesis PAH remains unclear.First, we explored function semaxanib' inhibitor VEGF receptor (SU5416)/hypoxia mice and monocrotaline-injected rats model. Then investigated smooth muscle cell aerobic glycolysis after being treated with supernatant. TGF (transforming growth factor)-βRI, pyruvate kinase 2, other antagonists were...
Hyperproliferation of pulmonary arterial smooth muscle cells (PASMCs) and consequent vascular remodeling are the crucial pathological features hypertension (PH). Protein methylation has been shown to be critically involved in PASMC proliferation PH, but underlying mechanism remains largely unknown.
A physiological membrane-receptor agonist typically stimulates oscillations, of varying frequencies, in cytosolic Ca2+ concentration ([Ca2+]i). Whether and how [Ca2+]i oscillation frequency regulates agonist-stimulated downstream events, such as gene expression, non-excitable cells remain unknown. By precisely manipulating histamine-stimulated vascular endothelial (ECs), we demonstrate that the expression cell adhesion molecule 1 (VCAM1) critically depends on presence, well absence,...
The initiation of hypoxic pulmonary vasoconstriction (HPV) involves an increase in cytosolic calcium ([Ca(2+)](i)) artery (PA) smooth muscle cells (PASMCs). Both the processes depend on extracellular Ca(2+). Extracellular Ca(2+) can be sensed by calcium-sensing receptor (CaSR). This study aims at determining whether CaSR is pivotal HPV.Experiments were performed cultured PASMCs, isolated PAs, and rats including knockdown preparations. hypoxia H(2)O(2) equivalent to level achieved increased...
Objective— This study aims to determine whether and how the enriched metabolites of endothelial extracellular vesicles (eEVs) are critical for cigarette smoke-induced direct injury cells development pulmonary hypertension, rarely explored in contrast long-investigated mechanisms secondary chronic hypoxemia. Approach Results— Metabonomic screen eEVs from cigarette-smoking human subjects reveals prominent elevation spermine—a polyamine metabolite with potent agonist activity CaSR...
Salmonella enterica serovar Newport (S Newport) infections are gradually on the rise in China from last decade. For humans' infections, S has been ranked among top five serovars responsible for persistent globally. A total of 290 S. strains with their relevant clinical metadata were analyzed, and subjected to whole-genome sequence analysis. Among these, 62.4% (n = 181) diarrheic patients 28.9% 84) asymptomatic individuals (including adults youngsters) while 8.6% 25) cases diarrhea infants...
This study introduced a novel dual fixation method for the pulmonary vasculature and lung tissue in hypertension (PH) rats, addressing limitations of traditional methods that failed to accurately preserve vivo status vascular morphology. The modified involved process, combining individualized ventilation support perfusion simulate respiratory motion, artery pressure right ventricular output rat under conditions. Utilizing monocrotaline-induced PH model, this compared with immersion fixation,...
Sedum alfredii Hance has been reported to be a Zn-hyperaccumulator plant species. In this study, root morphological and physiological response of the hyperaccumulating ecotype S. H. (HE) from mined area non-hyperaccumulating (NHE) agricultural supplied levels Zn Pb were investigated. The results showed that concentrations in leaves stems HE 34 41 times higher, whereas lead 1.9 2.4 greater, respectively, than those NHE when grown at 1224 microM and/or 200 Pb. At combined supply with Pb,...
[Ca(2+)](i) oscillations drive downstream events, like transcription, in a frequency-dependent manner. Why oscillation frequency regulates transcription has not been clearly revealed. A variation apparently leads to the time duration of cumulated elevations or spike duration. By manipulating duration, we generated series with same but different durations, as well frequencies Molecular assays demonstrated that, when 'artificial' models alone, under physiologically simulated conditions...
Overproduction of endothelial extracellular vesicles (eEVs) is correlated with pulmonary hypertension progression, but the precise mechanism remains largely unclear.MicroRNA-chip and real-time polymerase chain reaction were conducted to screen validate microRNA profiles in blood plasma eEVs rats human or without cigarette smoking. Pulmonary artery smooth muscle cells cultured study signaling pathways. phenotypes evaluated wild-type calcium-sensing receptor knockout identify...
Pulmonary vascular remodeling (PVR), encompassing microvascular loss and muscularization, contributes to multiple respiratory diseases. c-Kit+ cells exhibit differentiation potential into both endothelial (ECs) smooth muscle cells. The role of lung cell in PVR, however, remains unclear. Lung increase pulmonary hypertension patients the SU5416/hypoxia (SuHx)-induced PVR mouse model. Employing genetic lineage tracing single-cell RNA sequencing (scRNA-seq), we elucidate that lung-resident...
Agonist-stimulated [Ca2+]i oscillations are universally irregular in their kinetics. How dynamically regulate agonist-stimulated downstream events has not been studied. To overcome the obstacles of irregularity and heterogeneity oscillations, signaling NFκB/STAT3-GFP nuclear translocation were simultaneously monitored each single cell examined. The cause-effect relationship between oscillation parameters transcriptional activities was validated populations through with varied parameters....
Hypoxia-induced mitogenic factor (HIMF) is an inflammatory cytokine playing important role(s) in the development of hypoxic pulmonary hypertension. The molecular target mediating HIMF-stimulated downstream events remains unclear. coimmunoprecipitation screen identified extracellular calcium-sensing receptor (CaSR) as binding partner for HIMF artery smooth muscle cells. yeast 2-hybrid assay then revealed to intracellular, not extracellular, domain CaSR. enhanced activity CaSR and mediated...
The association of sex hormone (estradiol, testosterone, and progesterone) with cardiopulmonary disease has already attracted great attention, especially in pulmonary arterial hypertension (PAH). However, the impact hormones their pituitary stimulators (follicle-stimulating luteinizing hormone) on PAH men remains unclear. We conducted a prospective cohort study recruiting 95 patients idiopathic from 2008 to 2014 following up for median 65 months death. Compared control, abnormal plasma...
Hypoxia triggers pulmonary vasoconstriction, however induces relaxation of systemic arteries such as femoral arteries. Mitochondria are functionally and structurally heterogeneous between different cell types. The aim this study was to reveal whether mitochondrial heterogeneity controls the distinct responses versus artery smooth muscle cells hypoxia. Intact mitochondria were transplanted into Sprague-Dawley rat in culture vitro. retained functional after transplantation. cross...