A5058779705- Extracellular vesicles in disease
- Osteoarthritis Treatment and Mechanisms
- Medicinal Plant Pharmacodynamics Research
- Polysaccharides Composition and Applications
- Nanocomposite Films for Food Packaging
- Cancer Immunotherapy and Biomarkers
- Curcumin's Biomedical Applications
- Cervical Cancer and HPV Research
- Cancer, Hypoxia, and Metabolism
- Traditional Chinese Medicine Analysis
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Cardiac Ischemia and Reperfusion
- Cancer-related molecular mechanisms research
- Cardiovascular Disease and Adiposity
- Knee injuries and reconstruction techniques
- Lower Extremity Biomechanics and Pathologies
- Immune cells in cancer
- RNA modifications and cancer
- Polysaccharides and Plant Cell Walls
- PI3K/AKT/mTOR signaling in cancer
- Antimicrobial Peptides and Activities
- Veterinary Equine Medical Research
- Nephrotoxicity and Medicinal Plants
- Circular RNAs in diseases
Nanjing Medical University
2024
Beijing Forestry University
2024
Tianjin Medical University
2016-2023
Second Hospital of Tianjin Medical University
2023
Abstract Metabolic enzymes and metabolites display non-metabolic functions in immune cell signalling that modulate attack ability. However, whether how a tumour’s metabolic remodelling contributes to its resistance remain be clarified. Here we perform functional screen of genes rescue tumour cells from effector T cytotoxicity, identify the embryo- tumour-specific folate cycle enzyme methylenetetrahydrofolate dehydrogenase 2 (MTHFD2). Mechanistically, MTHFD2 promotes basal IFN-γ-stimulated...
Vascular endothelial dysfunction is the closely related determinant of ischemic heart disease (IHD). Endothelial and ischemia/reperfusion injury (IRI) have been associated with an increase in microvesicles (MVs) vivo. However, potential contribution (EMVs) to myocardial damage unclear. Here we aimed investigate role EMVs derived from hypoxia/reoxygenation (H/R) -treated human umbilical vein cells (HUVECs) on cultured H9c2 cardiomyocytes. H/R model was established induce HUVECs release...
The one-carbon metabolism enzyme methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is involved in the regulation of tumor oncogenesis and immune cell functions, but whether it can contribute to macrophage polarization remains elusive. Here, we show that MTHFD2 suppresses interferon-γ-activated macrophages (M(IFN-γ)) enhances interleukin-4-activated (M(IL-4)) both vitro vivo. Mechanistically, interacts with phosphatase tensin homolog (PTEN) suppress PTEN's phosphatidylinositol...
The immune checkpoint receptor, programmed cell death 1 (PD-1, encoded by PDCD1), mediates the escape of cancer, but whether PD-1 splicing isoforms contribute to this process is still unclear. Here, we identify an alternative isoform human PD-1, which carries a 28-base pairs extension retained from 5′ region intron 2 (PD-1^28), expressed in peripheral T cells and tumor infiltrating lymphocytes. PD-1^28 expression induced on upon activation regulated RNA binding protein, TAF15. Functionally,...
Abstract The protein O-GlcNAcylation catalysed by O-GlcNAc transferase (OGT) is tightly regulated glucose availability. It upregulated and essential for tumor cell proliferation under hypoxic conditions. However, the mechanism behind still unclear. Here, we showed that glycolytic regulator 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3), which also promotes cycle progression in nucleus, was O-GlcNAcylated response to hypoxia. of PFKFB3 could compete phosphorylation...
Osteochondral defects mostly occur as a result of trauma or articular degeneration. The poor regenerative ability cartilage remains osteochondral are tricky problem to deal with. modern treatment strategies mainly focus on tissue engineering with bioactive materials. In this study, we aimed develop icariin conditioned serum (ICS) together hyaluronic acid (HA) and determine their in reparing critical-sized defect rabbit knees.Primary chondrocytes were incubated at different concentrations,...
Abstract Background Osteochondral defects caused by an acute traumatic injury or articular degeneration remains difficult to be manipulated. Repair of is still a great challenge for both tissue engineers and orthopedic surgeons. Therefore, combination biomaterials with cartilage promotive drugs well worth being developed support the regeneration subchondral bone. Methods Rabbits undergoing osteochondral defect surgery were intrarticularly injected icariin-conditioned serum (ICS), chitosan...
Abstract Background Knee osteoarthritis (KOA) is one of the most common degenerative diseases. Its development closely related to cartilage injury and subchondral bone remodeling homeostasis. In present study, we combined icariin-conditioned serum (ICS) with thiolated chitosan (CSSH), a material widely used in tissue engineering for repair, demonstrate its effect on repair damage abnormal remodeling. Methods New Zealand rabbits were undergoing surgery defect, joint cavity injection was...
// Yao Wang 1 , Su Wei Yi-Lu Miao Liu Man Shang Qi Zhang 4 Yan-Na Wu Ming-Lin 2, 3 Jun-Qiu Song and Yan-Xia Department of Pharmacology, School Basic Medical Sciences, Tianjin University, 300070, China 2 Section Endocrinology, Medicine, Temple University Philadelphia, PA19140, USA PA 19140, Dermatology, Perelman Pennsylvania, 19104, Correspondence to: Liu, email: liu_yanxia126@126.com Song, song_junqiu@126.com Keywords: microvesicles, ischemia/reperfusion, apoptosis, Bcl-2/Bax, GRP78...
Background: Icariin, a traditional Chinese medicine, plays protective role in the treatment of exercise fatigue. Zinc, trace element, an important reproductive system. Therefore, we aimed to synthesize Icariin-Zinc complex (by chemical means) and verify its effect on fatigue system using animal experiments. Methods: The icariin-zinc was prepared by reaction icariin carbonyl zinc ions (molar ratio 1:3). molecular formula structural were identified tested. Fifty-six rats selected swimming...
To investigate the effects of circulating microvesicles (MVs) derived from ischemic preconditioning (IPC) on myocardial ischemia/reperfusion (I/R) injury in rats and explore underlying mechanism.To establish IPC model, were subjected to brief cycles left anterior descending (LAD) coronary occlusion reperfusion. The blood was drawn abdominal aorta once operation finished. IPC-MVs isolated by ultracentrifugation peripheral characterized flow cytometry. I/R model established vivo. Rats injected...
To investigate the effect of Iptakalim (Ipt) preventing injury endothelial microvesicles (EMVs) derived from hypoxia/reoxygenation (H/R)-treated HUVECs on relaxation rat thoracic aortic rings and explore underlying mechanism.H/R model was established to release H/R-EMVs HUVECs. were isolated by ultracentrifugation conditioned culture medium. characterized using Transmission Electron Microscope (TEM). Thoracic rats incubated with 10-7-10-3 mol/L Ipt co-cultured 10 μg/ml for 4 hours, their...