A5083670127- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Extracellular vesicles in disease
- Connective Tissue Growth Factor Research
- Pediatric health and respiratory diseases
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Respiratory and Cough-Related Research
- RNA Interference and Gene Delivery
- Allergic Rhinitis and Sensitization
- Biomarkers in Disease Mechanisms
- Sphingolipid Metabolism and Signaling
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- S100 Proteins and Annexins
- Air Quality and Health Impacts
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Microfluidic and Bio-sensing Technologies
- Nanopore and Nanochannel Transport Studies
- Hydrogen's biological and therapeutic effects
- Single-cell and spatial transcriptomics
- Synthesis and Biological Activity
Kyungpook National University
2024
University of Illinois Chicago
2018-2023
Brigham and Women's Hospital
2013-2020
Harvard University
2013-2020
Boston Medical Center
2016
Boston University
2016
Pohang University of Science and Technology
2009-2015
Johns Hopkins University
2009
Johns Hopkins Medicine
2009
North Carolina State University
2007
Gut microbiota play an important part in the pathogenesis of mucosal inflammation, such as inflammatory bowel disease (IBD). However, owing to complexity gut microbiota, our understanding roles commensal and pathogenic bacteria maintenance immune homeostasis is evolving only slowly. Here, we evaluated role their secreting extracellular vesicles (EV) development inflammation gut. Experimental IBD model was established by oral application dextran sulfate sodium (DSS) C57BL/6 mice. The...
Despite extensive research, the pathogenesis of cigarette smoking (CS)-associated emphysema remains incompletely understood, thereby impeding development novel therapeutics, diagnostics, and biomarkers. Here, we report a paradigm potentially involved in epithelial death tissue loss CS-associated emphysema. After prolonged exposure CS, CCN1 cleavage was detected both vitro vivo. Full-length (flCCN1) secreted an exosome-shuttled manner, plasmin converted flCCN1 to cleaved (cCCN1) extracellular...
Bacterial infection and its associated sepsis are devastating clinical entities that lead to high mortality morbidity in critically ill patients. Phagocytosis, along with other innate immune responses, exerts crucial impacts on the outcomes of these MicroRNAs (miRNAs) a novel class regulatory noncoding RNAs target specific mRNAs for modulation translation expression targeted protein. The roles miRNAs host defense against bacterial remain unclear. We found infections and/or bacterial-derived...
Cancer vaccines with optimal tumor-associated antigens show promise for anti-tumor immunotherapy. Recently, nano-sized vesicles, such as exosomes derived from tumors, were suggested potential antigen candidates, although the total yield of is not sufficient clinical applications. In present study, we developed a new vaccine strategy based on vesicles primary autologous tumors. Through homogenization and sonication tumor tissues, achieved high yields vesicle-bound antigens. These nanovesicles...
Chronic inflammatory airway diseases including asthma are characterized by immune dysfunction to inhaled allergens. Our previous studies demonstrated that T cell priming allergens requires LPS, which is ubiquitously present in household dust In this study, we evaluated the role of vascular endothelial growth factor (VEGF) development and its polarization Th1 or Th17 cells when exposed LPS-contaminated An mouse model was induced sensitization with then challenged alone. Therapeutic...
Inflammation involves in many cigarette smoke (CS) related diseases including the chronic obstructive pulmonary disease (COPD). Lung epithelial cell released IL-8 plays a crucial role CS induced lung inflammation. and extracts (CSE) both induce secretion subsequently, recruits inflammatory cells into parenchyma. However, molecular cellular mechanisms by which CSE triggers release remain not completely understood. In this study, we identified novel extracellular matrix (ECM) molecule, CCN1,...
Allergen-specific T-cell responses orchestrate airway inflammation, which is a characteristic of asthma. Recent evidence suggests that noneosinophilic asthma can be developed by mixed Th1 and Th17 cell when exposed to lipopolysaccharide (LPS)-containing allergens.To evaluate the therapeutic or adverse effects acetyl salicylic acid (ASA) on expression Th1-type Th17-type inflammation induced exposure LPS-containing allergens.Th1 + Th2 mouse models were generated intranasal sensitization with...
A new approach targeting aeroallergen sensing in the early events of mucosal immunity could have greater benefit. The CSF1-CSF1R pathway has a critical role trafficking allergens to regional lymph nodes through activating dendritic cells. Intervention this prevent allergen sensitization and subsequent Th2 allergic inflammation.To examine therapeutic effectiveness CSF1 CSF1R inhibition for blocking cell function aeroallergens.We adopted model chronic asthma induced by panel three naturally...
The resolution of inflammation is an active process coordinated by mediators and immune cells to restore tissue homeostasis. However, the mechanisms for resolving eosinophilic allergic lung triggered inhaled allergens have not been fully elucidated.
Abstract Endothelial barrier integrity is ensured by the stability of adherens junction (AJ) complexes comprised vascular endothelial (VE)-cadherin as well accessory proteins such β-catenin and p120-catenin. Disruption due to disassembly AJs results in tissue edema influx inflammatory cells. Using three-dimensional structured illumination microscopy, we observe that mitochondrial protein Mitofusin-2 (Mfn2) co-localizes at plasma membrane with VE-cadherin cells during homeostasis. Upon...
Recent clinical evidence indicates that the non-eosinophilic subtype of severe asthma is characterized by fixed airway obstruction, which may be related to emphysema. Transgenic studies have demonstrated high levels IFN-γ in airways induce Fibroblast growth factor 2 (FGF2), downstream mediator TGF-β, important wound healing. We investigated role FGF2 IFN-γ-induced emphysema and therapeutic effects recombinant prevention a model. To evaluate emphysema, lung targeted transgenic mice were...
Asthma is a chronic inflammatory airway disease driven by various infiltrating immune cell types into the lung. Optical microscopy has been used to study infiltrates in asthmatic lungs. Confocal laser scanning (CLSM) identifies phenotypes and locations of individual cells lung tissue sections employing high-magnification objectives multiplex immunofluorescence staining. In contrast, light-sheet fluorescence (LSFM) can visualize macroscopic mesoscopic architecture whole-mount tissues three...
Summary Background We recently demonstrated that the T‐helper type 1 (Th1) immune response plays an important role in development of non‐eosinophilic inflammation induced by airway exposure allergen plus double‐stranded RNA (dsRNA). However, lipoxygenase (LO) metabolites Th1 is poorly understood. Objective To evaluate LO sensitization with dsRNA. Methods A Th2‐allergic mouse model was created intraperitoneal injection lipopolysaccharide‐depleted ovalbumin (OVA, 75 μg) and alum (2 mg) twice,...
Abstract Formyl peptide receptors (FPRs) are chemoattractant that mediate inflammatory cell responses to infection. Recent evidence indicates noneosinophilic asthma phenotypes can be developed by both Th1 and Th17 when exposed LPS-containing allergens. In this study, we evaluated the effects of airway activation FPRs their synthetic agonist, Trp-Lys-Tyr-Met-Val-D-Met (W-peptide), on development in a mouse model. A model was generated intranasal sensitization with 10 μg LPS plus 75 OVA days...
Lung epithelial cell apoptosis is an important feature of hyperoxia-induced lung injury. The death receptor-associated extrinsic pathway and mitochondria-associated intrinsic both mediate the development apoptosis. Despite decades research, molecular mechanisms remain incompletely understood. Here, we report a novel regulatory paradigm in response to hyperoxia-associated oxidative stress. Hyperoxia markedly upregulated microRNA (miR)-15a/16 levels cells, bronchoalveolar lavage fluid (BALF)...
T-helper (Th)17 cell responses are important for the development of neutrophilic inflammatory disease. Recently, we found that acetyl salicylic acid (ASA) inhibited Th17 airway inflammation in an asthma mouse model induced by sensitization with lipopolysaccharide (LPS)-containing allergens. To investigate mechanism(s) inhibitory effect ASA on inflammation, a was generated intranasal LPS plus ovalbumin (OVA) and then challenged OVA alone. Immunologic parameters were evaluated 6 48 h after...