A5040737368- Ion channel regulation and function
- Pulmonary Hypertension Research and Treatments
- Mitochondrial Function and Pathology
- Cardiovascular Function and Risk Factors
- Neuroscience and Neuropharmacology Research
- ATP Synthase and ATPases Research
- Nicotinic Acetylcholine Receptors Study
- Pluripotent Stem Cells Research
- Caveolin-1 and cellular processes
- Mesenchymal stem cell research
- Cancer, Hypoxia, and Metabolism
- Pregnancy and preeclampsia studies
- Receptor Mechanisms and Signaling
- Birth, Development, and Health
- Neonatal Respiratory Health Research
- Cardiac electrophysiology and arrhythmias
- Reproductive System and Pregnancy
- Congenital heart defects research
- Cardiovascular Disease and Adiposity
- Pain Mechanisms and Treatments
- Angiogenesis and VEGF in Cancer
- Cardiovascular Issues in Pregnancy
- Neuroscience of respiration and sleep
- Immune Response and Inflammation
- Heme Oxygenase-1 and Carbon Monoxide
University of Illinois Chicago
2015-2024
Fluor (United States)
2021-2024
Center for Vascular Biology Research
2021-2024
Illinois College
2015-2023
University of Illinois Urbana-Champaign
2015-2023
University of Chicago
1995-2022
University of Illinois System
2022
Advanced Imaging Research (United States)
2021
Hospital São Paulo
2020
Indo-American Center
2015-2017
Mitochondria exist in dynamic networks that undergo fusion and fission. Mitochondrial fission are mediated by several GTPases the outer mitochondrial membrane, notably mitofusin-2 (Mfn-2), which promotes fusion, dynamin-related protein (Drp-1), We report human lung cancer cell lines exhibit an imbalance of Drp-1/Mfn-2 expression, a state Lung tumor tissue samples from patients demonstrated similar increase Drp-1 decrease Mfn-2 when compared to adjacent healthy lung. Complementary approaches...
Rationale: Pulmonary arterial hypertension (PAH) is a lethal syndrome characterized by pulmonary vascular obstruction caused, in part, artery smooth muscle cell (PASMC) hyperproliferation. Mitochondrial fragmentation and normoxic activation of hypoxia-inducible factor-1α (HIF-1α) have been observed PAH PASMCs; however, their relationship relevance to the development are unknown. Dynamin-related protein-1 (DRP1) GTPase that, when activated kinases that phosphorylate serine 616, causes...
Excessive proliferation and impaired apoptosis of pulmonary artery (PA) smooth muscle cells (PASMCs) contribute to vascular obstruction in patients fawn-hooded rats (FHRs) with PA hypertension (PAH). Expression activity mitochondrial superoxide dismutase-2 (SOD2), the major generator H(2)O(2), is known be reduced PAH; however, mechanism therapeutic relevance this are unknown.SOD2 expression PASMCs decreased PAH FHRs PAH. FHR have higher lower rates than Sprague-Dawley rat PASMCs. Moreover,...
Background The results from cross sectional and longitudinal studies show that periodontitis is closely associated with cognitive impairment (CI) Alzhemer's Disease (AD). Further, using animal model of human post-mortem brain tissues subjects AD strongly suggest a gram-negative periodontal pathogen, Porphyromonas gingivalis (Pg) and/or its product gingipain is/are translocated to the brain. However, neuropathology resulting Pg oral application not known. In this work, we tested hypothesis...
Blood vessels are lined by endothelial cells engaged in distinct organ-specific functions but little is known about their characteristic gene expression profiles. RNA-Sequencing of the brain, lung, and heart translatome identified specific pathways, transporters cell-surface markers expressed endothelium each organ, which can be visualized at http://www.rehmanlab.org/ribo. We found that express genes typically surrounding tissues such as synaptic vesicle brain cardiac contractile...
Human mesenchymal stem cells (MSCs) are adult multipotent which can be isolated from bone marrow, adipose tissue as well other tissues and have the capacity to differentiate into a variety of cell types such adipocytes, osteoblasts chondrocytes. Differentiation mature is guided by growth factors hormones, but recent studies suggest that metabolic shifts occur during differentiation modulate process. We therefore investigated mitochondrial biogenesis, respiration membrane potential adipogenic...
As essential regulators of mitochondrial quality control, dynamics and mitophagy play key roles in maintenance metabolic health cellular homeostasis. Here we show that knockdown the membrane-inserted scaffolding structural protein caveolin-1 (Cav-1) expression tyrosine 14 phospho-defective Cav-1 mutant (Y14F), as opposed to phospho-mimicking Y14D, altered morphology, increased matrix mixing, fusion fission well MDA-MB-231 triple negative breast cancer cells. Further, found interaction with...
Abstract It has previously been observed that expression of chemokine monocyte chemoattractant protein‐1 (MCP‐1/CC ligand 2 (CCL2)) and its receptor CC (CCR2) is up‐regulated by dorsal root ganglion (DRG) neurons in association with rodent models neuropathic pain. MCP‐1 increases the excitability nociceptive after a peripheral nerve injury, while disruption MCP‐1/CCR2 signaling blocks development pain, suggesting responsible for heightened pain sensitivity. To define mechanisms DRG, we...
Pulmonary arterial hypertension (PAH) is a lethal, female-predominant, vascular disease. Pathologic changes in PA smooth muscle cells (PASMC) include excessive proliferation, apoptosis-resistance, and mitochondrial fragmentation. Activation of dynamin-related protein increases mitotic fission promotes this proliferation-apoptosis imbalance. The contribution decreased fusion reduced mitofusin-2 (MFN2) expression to PAH unknown.We hypothesize that MFN2 fragmentation, impairs apoptosis. role...
The etiology of hepatopulmonary syndrome (HPS), a common complication cirrhosis, is unknown. Inflammation and macrophage accumulation occur in HPS; however, their importance unclear. Common bile duct ligation (CBDL) creates an accepted model HPS, allowing us to investigate the cause HPS.
Pulmonary arterial hypertension (PAH) is a proliferative arteriopathy associated with glucose transporter-1 (Glut1) up-regulation and glycolytic shift in lung metabolism. Glycolytic metabolism can be detected the positron emission tomography (PET) tracer (18)F-fluorodeoxyglucose (FDG).The precise cell type which abnormalities occur PAH unknown. Moreover, whether FDG-PET sufficiently sensitive to monitor progression detect therapeutic regression untested. We hypothesized that increased...
Hypercapnia (elevated CO2 levels) occurs as a consequence of poor alveolar ventilation and impairs fluid reabsorption (AFR) by promoting Na,K-ATPase endocytosis. We studied the mechanisms regulating CO2-induced endocytosis in epithelial cells (AECs) dysfunction rats. Elevated levels caused rapid activation AMP-activated protein kinase (AMPK) AECs, key regulator metabolic homeostasis. Activation AMPK was mediated CO2-triggered increase intracellular Ca2+ concentration...
Highlights•PDI functions as a thiol reductase for mitochondrial fission protein Drp1•Loss of PDI induces Drp1 sulfenylation at Cys644, driving endothelial senescence•Dysfunction contributes to impaired wound healing in diabetes•Restoring PDI-Drp1 axis protects against diabetic vascular complicationSummaryMitochondrial dynamics are tightly controlled by fusion and fission, their dysregulation excess reactive oxygen species (ROS) contribute cell (EC) dysfunction. How redox signals regulate...
Abstract Repair of the endothelial cell barrier after inflammatory injury is essential for tissue fluid homeostasis and normalizing leukocyte transmigration. However, mechanisms regeneration remain poorly understood. Here we show that hematopoietic developmental transcription factor Sox17 promotes in endotoxemia model injury. Genetic lineage tracing studies demonstrate native endothelium itself serves as primary source cells repopulating vessel wall following We identify a key regulator...
The cause and consequences of impaired adrenergic signaling in right ventricular failure/hypertrophy (RVH) are poorly understood. We hypothesized that G protein-coupled receptor kinase-2 (GRK2)-mediated uncoupling β-adrenergic impairs inotropic reserve. implications (RV) remodeling for inotrope selection the therapeutic benefit interrupting Gβγ-GRK2 interaction, using gallein, were tested.Chamber-specificity cellular localization compared rodent RVH associated with pulmonary arterial...
Caveolin 1 (Cav1) is a required structural component of caveolae, and its phosphorylation by Src associated with an increase in caveolae-mediated endocytosis. Here we demonstrate, using quantitative live-cell 4D, TIRF, FRET imaging, that endocytosis trafficking caveolae are Cav1 Tyr-14 phosphorylation-dependent conformational change, which spatially separates, or loosens, molecules within the oligomeric caveolar coat. When tracked TIRF spinning-disk microscopy, cells expressing...
It has been hypothesized that R-type Ca currents result from the expression of alpha(1E) gene. To test this hypothesis we examined properties voltage-dependent channels in mice which channel subunit had deleted. Application omega-conotoxin GVIA, omega-agatoxin IVA, and nimodipine to cultured cerebellar granule neurons wild-type inhibited components whole-cell Ba current, leaving a "residual" R current with an amplitude approximately 30% total current. A minor portion was by...
The present study investigated 1) whether extra- and intramyometrial arteries contain hCG/human LH receptor messenger ribonucleic acid (mRNA) protein, 2) hCG can bind to its vascular receptors regulate the formation of vasoactive eicosanoids, 3) administration for ovulation induction affect resistance in uterine arteries. multiple hCG/LH mRNA transcripts endothelial smooth muscle cells. also an 80-kilodalton immunoreactive protein [125I]hCG, which is inhibited by excess unlabeled hCG. mRNA,...
The proinflammatory cytokine S100A12 is associated with coronary atherosclerotic plaque rupture. We previously generated transgenic mice vascular smooth muscle-targeted expression of human and found that these developed aortic aneurysmal dilation the thoracic aorta. In current study, we tested hypothesis expressed in muscle atherosclerosis-prone apolipoprotein E (ApoE)-null would accelerate atherosclerosis.ApoE-null or without transgene were analyzed. a 1.4-fold increase size more...