A5080268053- Advanced Fluorescence Microscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced Electron Microscopy Techniques and Applications
- Advanced Biosensing Techniques and Applications
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Near-Field Optical Microscopy
- Caveolin-1 and cellular processes
- Protein Structure and Dynamics
- Sarcoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Receptor Mechanisms and Signaling
- Pain Mechanisms and Treatments
- Nicotinic Acetylcholine Receptors Study
- Protein purification and stability
- Force Microscopy Techniques and Applications
- Antimicrobial Peptides and Activities
University of Illinois Chicago
2020-2024
Liberal Arts University
2023
As essential regulators of mitochondrial quality control, dynamics and mitophagy play key roles in maintenance metabolic health cellular homeostasis. Here we show that knockdown the membrane-inserted scaffolding structural protein caveolin-1 (Cav-1) expression tyrosine 14 phospho-defective Cav-1 mutant (Y14F), as opposed to phospho-mimicking Y14D, altered morphology, increased matrix mixing, fusion fission well MDA-MB-231 triple negative breast cancer cells. Further, found interaction with...
Dendritic cells (DCs) can infiltrate tight junctions of the epithelium to collect remote antigens during immune surveillance. While elongated membrane structures represent a plausible structure perform this task, their functional mechanisms remain elusive owing lack high-resolution characterizations in live DCs. Here, we developed fluorescent artificial (FAAs) based on quantum dots coated with polyacrylic acid. Single-particle tracking FAAs enables us superresolve fiber network responsible...
We present phalloidin-based points accumulation for imaging in nanoscale topography (phalloidin-PAINT), enabling quantitative superresolution of filamentous actin (F-actin) the cell body and delicate membrane protrusions. demonstrate that intrinsic phalloidin dissociation enables PAINT microscopy an buffer containing low concentrations dye-conjugated phalloidin. further show enhanced single-molecule labeling by chemically promoting dissociation. Two benefits phalloidin-PAINT are its ability...
We present a versatile single-molecule localization microscopy technique utilizing time-lapse imaging of single-antibody labeling. By performing in the subminute time scale and tuning antibody concentration to create sparse binding, we captured labeling subcellular targets generate superresolution images. Single-antibody enabled dual-target using dye-conjugated monoclonal polyclonal antibodies. further demonstrate dual-color strategy increase sample density. paves new way evaluate binding...
Antibody–antigen interactions represent one of the most exploited biomolecular in experimental biology. While numerous techniques harnessed immobilized antibodies for nanoscale fluorescence imaging, few utilized their reversible binding kinetics. Here, we investigated noncovalent monoclonal hemagglutinin (HA) epitope tag antibody, 12CA5, fixed cellular environment. We observed that use a chaotropic agent, potassium thiocyanate (KSCN), promoted dissociation 12CA5 antibody fragment (Fab),...
Chronic neuropathic pain is an increasingly prevalent societal issue that responds poorly to existing therapeutic strategies. The α9α10 nicotinic acetylcholine receptor (nAChR) has emerged as a potential target treat pain. However, challenges in expressing functional nAChRs mammalian cell lines have slowed the discovery of ligands and studies into relationship between Here, we develop line HEK293 background stably expresses nAChRs. By also developing only α9 α10 subunits, identify distinct...
Abstract In single‐molecule localization microscopy (SMLM), immunofluorescence (IF) staining affects the quality of reconstructed superresolution images. However, optimizing IF remains challenging because is a one‐step, irreversible process. Sample labeling through reversible binding presents an alternative strategy, but such techniques require significant technological advancements to enhance dissociation labels without sacrificing their specificity. this article, we introduce time‐lapse...
ABSTRACT We present single-molecule labeling and localization microscopy (SMLLM) using dye-conjugated phalloidin to achieve enhanced superresolution imaging of filamentous actin (F-actin). demonstrate that the intrinsic dissociation enables SMLLM in an buffer containing low concentrations phalloidin. further show by chemically promoting dissociation. Two benefits phalloidin-based are better preservation cellular structures sensitive mechanical shear forces during standard sample preparation...
Single-molecule imaging has provided new insights on weak transient biomolecular interactions with micromolar to millimolar affinity. However, the limited duration of observation hindered study strong and reversible sub-nanomolar We report single-molecule interaction microscopy (SMIM), which combines point accumulation for in nanoscale topography (PAINT) extended durations that enables antibody binding kinetics cellular environment. SMIM revealed heterogeneous effect concentration valency...