A5004814346- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- Ion channel regulation and function
- Nursing Diagnosis and Documentation
- Anatomy and Medical Technology
- Biomedical and Engineering Education
- Nicotinic Acetylcholine Receptors Study
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Inflammasome and immune disorders
- Posttraumatic Stress Disorder Research
- Hepatitis C virus research
- Immune responses and vaccinations
- X-ray Diffraction in Crystallography
- Social Media in Health Education
- Catalysis and Oxidation Reactions
- vaccines and immunoinformatics approaches
- Cytomegalovirus and herpesvirus research
- Cardiac electrophysiology and arrhythmias
- Machine Learning in Materials Science
- CAR-T cell therapy research
- Disaster Response and Management
- interferon and immune responses
Australian Regenerative Medicine Institute
2020-2025
Monash University
2018-2025
University of South Florida
2025
Texas A&M University
2025
Liverpool Hospital
2022-2024
Western University
2023-2024
CSIRO Manufacturing
2024
Stanford Medicine
2024
Louisiana State University Health Sciences Center Shreveport
2024
Discovery Institute
2024
The voltage-gated potassium channel in T lymphocytes, Kv1.3, is an important molecular target for immunosuppressive agents. A structurally defined polypeptide, ShK, from the sea anemone Stichodactyla helianthus inhibited Kv1.3 potently and also blocked Kv1.1, Kv1.4, Kv1.6 at subnanomolar concentrations. Using mutant cycle analysis conjunction with complementary mutagenesis of ShK utilizing structure we determined a likely docking configuration this peptide channel. Based upon topological...
Making sense of TCR–pMHC topology Most T cells use a cell receptor (TCR) that recognizes major histocompatibility complex molecules bound to peptides (pMHCs) derived from both self- and foreign antigens. Although there is great variability in the interface because diversity partners, this interaction displays canonical docking for reasons remain contested. Zareie et al. tested an assortment reversed-polarity TCRs were all specific same cognate pMHC-I bearing peptide influenza A virus (IAV)...
The crosstalk between the host's inflammasome system and invading virulent/less-virulent viruses determines outcome of ensuing inflammatory response. An appropriate activation inflammasomes triggers antiviral responses that clear virus heal inflamed tissue. However, an aberrant can result in a harmful overwhelming inflammation could damage infected underlying immune mechanisms viral virulent factors impact severe clinical disease remain to be fully elucidated. In this study, we used herpes...
The nonpeptide agent CP-339,818 (1-benzyl-4-pentylimino-1,4-dihydroquinoline) and two analogs (CP-393,223 CP-394,322) that differ only with respect to the type of substituent at N1 position, potently blocked Kv1.3 channel in T lymphocytes. A fourth compound (CP-393,224), which has a smaller less-lipophilic group N1, was 100-200-fold less potent, suggesting large lipophilic this position is necessary for drug activity. from outside IC50 value approximately 200 nM 1:1 stoichiometry...
Abstract Objectives Social media platforms like Facebook, X (formally Twitter), and Instagram bridge pathology programs with other health professionals, prospective students, the public, but extent of social usage by residency remains unexplored. This study investigates current landscape utilization programs. Methods Using National Resident Matching Program (NRMP) Match Data from 2022, 139 anatomic clinical were analyzed categorized into 3 prestige tiers based on Doximity ratings. There...
Women's autonomy plays a critical role in decision-making of health service use. This study aims to evaluate the relationship between Human Immunodeficiency Virus (HIV) testing and among Cambodian women aged 15-49. We used data from 2021-2022 Cambodia Demographic Health Survey (DHS) our sample consisted currently married/cohabiting 15-49 (N = 13,755). The outcome variable was "ever been tested for HIV." Covariates were household reproductive scores, socio-demographic characteristics (age,...
The interaction between the CD4/CD8 co-receptors and LCK (an Src family tyrosine kinase) is thought to augment T cell activation upon recognition of peptide-loaded major histocompatibility complexes (pMHCs). How this influences antigen-specific development unclear however, as its impact on naïve immune repertoires. In mice expressing mutated endogenous unable bind (LCK FREE mice), we show that influenza A virus (IAV)–derived pMHC-specific CD8 CD4 responses had a significantly narrowed...
We report the isolation of a novel mouse voltage-gated <i>Shaker</i>-related K<sup>+</sup> channel gene,<i>Kv1.7</i> (<i>Kcna7/KCNA7</i>). Unlike other known Kv1 family genes that have intronless coding regions, protein-coding region <i>Kv1.7</i> is interrupted by 1.9-kilobase pair intron. The gene and related <i>Kv3.3</i>(<i>Kcnc3/KCNC3</i>) map to chromosome 7 human 19q13.3, has been suggested contain diabetic susceptibility locus. Kv1.7 voltage-dependent rapidly inactivating, exhibits...
HSV-specific tissue-resident memory CD8 + T RM cells play a critical role in preventing virus reactivation from latently infected TG and subsequent shedding tears that trigger the recurrent corneal herpetic disease. In this report, we determined how dual blockade of PD-1 LAG-3 immune checkpoints, combined with vaccination, improved function associated significant reduction ocular herpes HLA transgenic (Tg) rabbit model. The appeared to have synergistic effect generating frequent...
UK‐78,282, a novel piperidine blocker of the T lymphocyte voltage‐gated K + channel, Kv1.3, was discovered by screening large compound file using high‐throughput 86 Rb efflux assay. This blocks Kv1.3 with IC 50 ∼200 n M and 1 : stoichiometry. A closely related compound, CP‐190,325, containing benzyl moiety in place benzhydryl is significantly less potent. Three lines evidence indicate that UK‐78,282 inhibits use‐dependent manner preferentially blocking binding to C‐type inactivated state...
The voltage-gated K+ channel of T-lymphocytes, Kv1.3, was heterologously expressed in African Green Monkey kidney cells (CV-1) using a vaccinia virus/T7 hybrid expression system; each infected cell exhibited 10(4) to 5 x 10(5) functional channels on the surface. protein, solubilized with detergent (3-[cholamidopropyl)dimethylammonio]-1-propanesulfonic acid or cholate), purified near-homogeneity by single nickel-chelate chromatography step. Kv1.3 protein virus-infected and its counterpart are...
Exposure to the September 11, 2001 (9/11) terrorist attacks has been found be associated with posttraumatic stress disorder (PTSD) and comorbid PTSD depression up 10-11 years post-disaster. However, little is known about longitudinal predictors of mental health conditions over time.We examined determinants within strata among 21,258 enrollees World Trade Center Health Registry who completed four questionnaires 14 follow-up (Wave 1 in 2003-04; Wave 2 2005-06; 3 2011-12; 4 2015-16). status was...
CD8 virtual memory T (TVM) cells are Ag-naive that have undergone partial differentiation in response to common γ-chain cytokines, particularly IL-15 and IL-4. TVM from young individuals highly proliferative TCR cytokine stimulation but, with age, they lose TCR-mediated capacity exhibit hallmarks of senescence. Helminth infection can drive an increase cells, which is associated improved pathogen clearance during subsequent infectious challenge mice. Given the cytokine-dependent profile their...
Burgeoning evidence demonstrates that responses to environmental exposures can be transmitted subsequent generations through the germline without DNA mutations 1,2 . This is controversial because underlying mechanisms remain identified. Therefore, understanding how effects of are unexposed a fundamental unanswered question in biology. Here, we used an established murine model transgenerational obesity show direct or ancestral exposure obesogen tributyltin (TBT) elicited persistent changes...
The immune co-receptor CD8 molecule (CD8) has two subunits, CD8α and CD8β, which can assemble into homo or heterodimers. Nonclassical (class-Ib) major histocompatibility complex (MHC) molecules (MHC-Ibs) have recently been identified as ligands for the CD8αα homodimer. This was demonstrated by observation that 2, Q region locus 10 (H2-Q10) is a high-affinity ligand also binds MHC-Ib H2-TL. suggests proteins may be an extended source of ligands. Expression H2-T3/TL H2-Q10 restricted to small...
We investigated the protective role of iNKT cell subsets in asymptomatic ocular herpesvirus infection. found that early during infection (i.e., on day 2 postinfection), IFN-γ-producing PLZF lo RORγt iNKT1 cells were predominant subset infected corneas B6 mice (with little to no corneal herpetic disease), compared symptomatic severe disease). Moreover, wild-type (WT) mice, CD1d KO which lack cells, showed (i) decreases levels IFN-γ and IL-12, (ii) an increase level inflammatory cytokine IL-6;...
Chronic neuropathic pain is an increasingly prevalent societal issue that responds poorly to existing therapeutic strategies. The α9α10 nicotinic acetylcholine receptor (nAChR) has emerged as a potential target treat pain. However, challenges in expressing functional nAChRs mammalian cell lines have slowed the discovery of ligands and studies into relationship between Here, we develop line HEK293 background stably expresses nAChRs. By also developing only α9 α10 subunits, identify distinct...