A5019064609- Allergic Rhinitis and Sensitization
- Gut microbiota and health
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Food Allergy and Anaphylaxis Research
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Diet and metabolism studies
- Dermatology and Skin Diseases
- IL-33, ST2, and ILC Pathways
- Helicobacter pylori-related gastroenterology studies
- Contact Dermatitis and Allergies
- Probiotics and Fermented Foods
- Autophagy in Disease and Therapy
- Pharmacological Effects and Assays
- T-cell and Retrovirus Studies
- Inflammasome and immune disorders
- RNA Interference and Gene Delivery
- Biochemical Analysis and Sensing Techniques
- Spondyloarthritis Studies and Treatments
- Systemic Lupus Erythematosus Research
- Ginseng Biological Effects and Applications
- SARS-CoV-2 and COVID-19 Research
- Pancreatic function and diabetes
Monash University
2015-2025
Murdoch Children's Research Institute
2025
Australian Regenerative Medicine Institute
2016-2021
Melbourne Clinic
2020
Jeffrey Modell Foundation
2019
Diet and the gut microbiota may underpin numerous human diseases. A major metabolic product of commensal bacteria are short-chain fatty acids (SCFAs) that derive from fermentation dietary fibre. Here we show diets deficient or low in fibre exacerbate colitis development, while very high intake SCFA acetate protects against colitis. SCFAs binding to 'metabolite-sensing' receptors GPR43 GPR109A non-haematopoietic cells mediate these protective effects. The inflammasome pathway has hitherto...
Asthma is prevalent in Western countries, and recent explanations have evoked the actions of gut microbiota. Here we show that feeding mice a high-fibre diet yields distinctive microbiota, which increases levels short-chain fatty acid, acetate. High-fibre or acetate-feeding led to marked suppression allergic airways disease (AAD, model for human asthma), by enhancing T-regulatory cell numbers function. Acetate acetylation at Foxp3 promoter, likely through HDAC9 inhibition. Epigenetic effects...
The incidence of food allergies in western countries has increased dramatically recent decades. Tolerance to antigens relies on mucosal CD103(+) dendritic cells (DCs), which promote differentiation regulatory T (Treg) cells. We show that high-fiber feeding mice improved oral tolerance and protected from allergy. High-fiber reshaped gut microbial ecology the release short-chain fatty acids (SCFAs), particularly acetate butyrate. enhanced against allergy by enhancing retinal dehydrogenase...
While treatment for atopic rhinitis is aimed mostly to relieve symptoms, only allergen-specific immunotherapy (AIT) targeted modify the natural history of allergic diseases. This results in sustained clinical tolerance, even when has stopped. The immunomodulatory effects AIT are attributed mainly increased regulatory T-cell function and IgG4 , yet little known about effect on memory B-cell compartment.We examine IgE- IgG subclass-expressing B cells.We recruited 29 patients with seasonal...
Abstract The proliferation and differentiation of antigen‐specific B cells, including the generation germinal centers (GC), are prerequisites for long‐lasting, antibody‐mediated immune protection. Affinity antigen determines cell recruitment, proliferation, differentiation, competitiveness in response, largely through determining access to T help. However, how cell‐derived signals contribute these outcomes is incompletely understood. Here, we report signature cytokine follicular helper...
Abstract Background Sublingual immunotherapy (SLIT) for grass pollen allergy can modify the natural history of allergic rhinitis and is associated with increased allergen‐specific IgG 4 . competitively inhibits functional IgE on surface effector cells, such as mast cells basophils, from binding to allergens. To further understand important role memory B‐cell (Bmem) responses play in mediating beneficial effects SLIT, we assessed changes Bmem subsets induced by SLIT allergy. Methods Blood...
Abstract Background Signal transducer and activator of transcription 3 hyper‐IgE syndrome (STAT3‐HIES) is caused by heterozygous mutations in the STAT3 gene associated with eczema, elevated serum IgE, recurrent infections resembling severe atopic dermatitis, while clinically relevant specific IgE almost absent. Methods To investigate impact signaling on B‐cell responses, we assessed lymph node bone marrow, blood B plasma cell subsets, somatic hypermutations Ig genes, vitro proliferation...
During gastrointestinal infection, dysbiosis can result in decreased production of microbially derived short-chain fatty acids (SCFAs). In response to the presence intestinal pathogens, we examined whether an engineered acetate- or butyrate-releasing diet rectify deficiency SCFAs and lead resolution enteric infection.We tested a high butyrate-producing (HAMSA HAMSB, respectively) condition Citrobacterrodentium infection mice assess its impact on host-microbiota interactions. We analysed...
Abstract Humoral immune responses require germinal centres (GC) for antibody affinity maturation. Within GC, B cell proliferation and mutation are segregated from affinity-based positive selection in the dark zone (DZ) light (LZ) substructures, respectively. While IL-21 is known to be important maturation GC maintenance, here we show it required both establishing normal representation preventing accumulation of cells G1 cycle stage LZ. Cell progression DZ unaffected by availability, as...
pH sensing by GPR65 regulates various inflammatory conditions, but its role in skin remains unknown. In this study, we performed a phenome-wide association study and report that the T allele of GPR65-intronic single-nucleotide polymorphism rs8005161, which reduces signaling, showed significant with atopic dermatitis, addition to bowel diseases asthma, as previously reported. Consistent genetic humans, show deficiency mice resulted markedly exacerbated disease MC903 experimental model...
The proliferation marker Ki67 has been attributed critical functions in maintaining mitotic chromosome morphology and heterochromatin organization during the cell cycle, indicating a potential role developmental processes requiring rigid cell-cycle control. Here, we discovered that despite normal fecundity organogenesis, germline deficiency resulted substantial defects specifically peripheral B T lymphocytes. This was not due to impaired but rather early lymphopoiesis at specific stages...
Allergen immunotherapy (AIT) is the only disease-modifying treatment for allergic disorders. We have recently discovered that allergen-specific memory B-cells (Bmem) are phenotypically altered after 4 months sublingual AIT ryegrass pollen allergy. Whether these effects shared with subcutaneous (SCIT) and affect epitope-specificity of Bmem remain unknown.