A5028596716- Congenital heart defects research
- Atherosclerosis and Cardiovascular Diseases
- Bioinformatics and Genomic Networks
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- Gene Regulatory Network Analysis
- RNA modifications and cancer
- Immune cells in cancer
- Zebrafish Biomedical Research Applications
- Mesenchymal stem cell research
- Medical Imaging and Pathology Studies
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- S100 Proteins and Annexins
- Phagocytosis and Immune Regulation
- Angiogenesis and VEGF in Cancer
- Neonatal Respiratory Health Research
Guardant (United States)
2023-2025
University of Illinois Chicago
2017-2021
Tempus Labs (United States)
2021
Illinois College
2017-2020
University of Illinois Urbana-Champaign
2017-2020
Indiana University School of Medicine
2017
Indiana University – Purdue University Indianapolis
2017
Blood vessels are lined by endothelial cells engaged in distinct organ-specific functions but little is known about their characteristic gene expression profiles. RNA-Sequencing of the brain, lung, and heart translatome identified specific pathways, transporters cell-surface markers expressed endothelium each organ, which can be visualized at http://www.rehmanlab.org/ribo. We found that express genes typically surrounding tissues such as synaptic vesicle brain cardiac contractile...
Abstract Repair of the endothelial cell barrier after inflammatory injury is essential for tissue fluid homeostasis and normalizing leukocyte transmigration. However, mechanisms regeneration remain poorly understood. Here we show that hematopoietic developmental transcription factor Sox17 promotes in endotoxemia model injury. Genetic lineage tracing studies demonstrate native endothelium itself serves as primary source cells repopulating vessel wall following We identify a key regulator...
Abstract Introduction: Next-generation sequencing (NGS) of tumor tissue is an integral technique to identify actionable alterations and inform oncology treatment. While established, NGS limited by variability in quantity quality both samples test platforms, which limits its effectiveness. A robust workflow with high sample success rate, providing comprehensive molecular profiling, fast turn-around time can improve this technology support patient care. Here, we report the analytical...
The mechanisms underlying the dedifferentiation and lineage conversion of adult human fibroblasts into functional endothelial cells have not yet been fully defined. Furthermore, it is known whether fibroblast recapitulates generation multipotent progenitors during embryonic development, which give rise to hematopoietic cell lineages. Here we established role developmental transcription factor SOX17 in regulating bilineage by intermediate progenitors.CD34+ were generated after dermal...
Abstract Endothelial barrier integrity is ensured by the stability of adherens junction (AJ) complexes comprised vascular endothelial (VE)-cadherin as well accessory proteins such β-catenin and p120-catenin. Disruption due to disassembly AJs results in tissue edema influx inflammatory cells. Using three-dimensional structured illumination microscopy, we observe that mitochondrial protein Mitofusin-2 (Mfn2) co-localizes at plasma membrane with VE-cadherin cells during homeostasis. Upon...
The heterogeneity of cells across tissue types represents a major challenge for studying biological mechanisms as well therapeutic targeting distinct tissues. Computational prediction tissue-specific gene regulatory networks may provide important insights into the underlying cellular in organs and Using three pathway analysis techniques, set enrichment (GSEA), parametric (PGSEA), alongside our novel model (HeteroPath), which assesses heterogeneously upregulated downregulated genes within...
Abstract Background: Despite its revolutionary impact, cancer genomics alone provides little information on tumor phenotype or functional state, which are governed by epigenetic mechanisms, notably methylation of regulatory regions. Tumor and host signatures reflect not only phenotype, such as histology, prognosis, protein expression, sub-type, but also that the microenvironment patient, including immune status, therapy-related adverse events, comorbidities, disease location. Epigenetic...
Abstract Tumor genome sequencing has emerged as a powerful tool for identifying biomarkers targeted cancer therapies. While DNA is well-established method and considered gold standard, RNA (RNA-seq) can identify anomalies in gene transcription, regulation of expression, fusions, which have critical diagnostic therapeutic impacts. Tempus Labs CLIA-certified, CAP-accredited offers several clinically validated NGS assays solid tumor hematological malignancy testing, including the xT 648-gene...
Computational analysis of tissue-specific gene expression may provide important insights into disease mechanisms and organ specific therapeutic targets. Here we applied our novel approach, HeteroPath, to characterize endothelial cell (EC) heterogeneity from an inherently unbiased perspective. Endothelial cells (ECs) in distinct vascular beds exhibit significant structure function as well propensity for disease. Understanding the molecular basis signatures networks bed-specific mechanisms. In...
The mechanisms underlying the de-differentiation and lineage conversion of adult human fibroblasts into functional endothelial cells have not yet been fully defined. Furthermore, it is known whether fibroblast recapitulates generation multipotent progenitors during embryonic development which give rise to hematopoietic cell lineages. Here we established role developmental transcription factor Sox17 in regulating bi-lineage via intermediate progenitor cells. CD34+ were generated following...