A5101458892- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Aquaculture disease management and microbiota
- Biochemical and Structural Characterization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Animal Virus Infections Studies
- Streptococcal Infections and Treatments
- Botanical Research and Chemistry
- Aquaculture Nutrition and Growth
- Invertebrate Immune Response Mechanisms
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Bone and Dental Protein Studies
- Viral Infections and Vectors
- Microbial Inactivation Methods
- CRISPR and Genetic Engineering
- Nutrition, Health and Food Behavior
- Marine Bivalve and Aquaculture Studies
- Inflammasome and immune disorders
- Diverse Approaches in Healthcare and Education Studies
- Catalysts for Methane Reforming
- Rabies epidemiology and control
- Atmospheric and Environmental Gas Dynamics
University of Chicago
2008-2025
Gwangju Institute of Science and Technology
2020-2025
Tufts University
2025
Chonnam National University
2012-2020
Korea Institute of Science and Technology
2019
Argonne National Laboratory
2011-2016
Seoul National University
2007
John Innes Centre
1997
Massachusetts Institute of Technology
1989
In various bacteria, Zur, a zinc-specific regulator of the Fur family, regulates genes for zinc transport systems to maintain homeostasis. It has also been suggested that Zur controls mobilization by regulating some ribosomal proteins. The antibiotic-producing soil bacterium Streptomyces coelicolor contains four family regulators, and one (named zur) is located downstream znuACB operon encoding putative uptake transporter. We found specifically repressed level znuA transcripts this was...
The cause of chronic inflammatory periodontitis, which leads to the destruction periodontal ligament and alveolar bone, is multifactorial. An increasing number studies have shown clinical significance NLRP3-mediated low-grade inflammation in degenerative disorders, but its causal linkage age-related periodontitis has not yet been elucidated. In this study, we investigated involvement NLRP3 inflammasome therapeutic potential inhibition bone loss by using vivo vitro models. poor quality bones...
Using Streptomyces coelicolor A3(2) protoplasts, the number of transformants obtained by homologous recombination incoming double-stranded circular DNA with recipient chromosome was greatly stimulated simple denaturation donor DNA. This procedure very effective inserts over a ca. 100-fold size range, largest tested being 40-kb in cosmids. These observations led to transformation experiments linearized cloned and randomly sheared genomic In both cases, significant levels transformation. Most...
Summary Bacillus cereus G9241 causes an anthrax‐like respiratory illness in humans; however, the molecular mechanisms of disease pathogenesis are not known. Genome sequencing identified two putative virulence plasmids proposed to provide for anthrax toxin (pBCXO1) and/or capsule expression (pBC218). We report here that B. immune‐competent mice, which is dependent on each plasmids. pBCXO1 encodes pagA1 , homologue protective antigen, as well hasACB providing hyaluronic acid formation, traits...
ABSTRACT In bacteria, signal peptides direct pre-proteins to the SecYEG secretion channel and are typically cleaved by peptidases during translocation across membrane. gram-positive such as Staphylococcus aureus , some have a pre-translocation function. Staphylococcal protein A (SpA) carries an atypical sequence, with YSIRK/GXXS motif that directs its precursor into cross-wall of dividing cells for subsequent anchoring sortase A. Here, we report PepV—a member M20 peptidase family which has...
Bacillus anthracis grows in chains of rod-shaped cells, a trait that contributes to its escape from phagocytic clearance host tissues. Using genetic approach search for determinants B. chain length, we identified mutants with insertional lesions secA2. All isolated secA2 exhibited an exaggerated whereas the dimensions individual cells were not changed. Complementation studies revealed slaP (S-layer assembly protein), gene immediately downstream on chromosome, is also determinant length. Both...
ATF6 is an endoplasmic reticulum (ER) membrane-bound transcription factor that regulates various cellular functions. The purpose of this study was to investigate the role in odontoblast differentiation. Rat tooth germs were isolated, changes gene expression evaluated over time, and localization determined by immunohistochemistry. Human dental pulp cells (HDPCs) cultured with 50 µg/mL ascorbic acid 5 mmol/L β-glycerophosphate or 100 ng/mL bone morphogenetic protein 2 induce Translocation...
Assembly of pili in Gram-positive bacteria and their attachment to the cell wall envelope are mediated by sortases. In Bacillus cereus its close relative anthracis, major pilin protein BcpA is cleaved between threonine glycine C-terminal LPXTG motif sorting signal pilin-specific sortase D. The resulting acyl enzyme intermediate relieved nucleophilic attack side-chain amino group lysine within YPKN another subunit. Cell anchoring assembled requires A, which also cleaves residues. We show here...
Bacillus cereus and other Gram-positive bacteria elaborate pili via a sortase D-catalyzed transpeptidation mechanism from major minor pilin precursor substrates. After cleavage of the LPXTG sorting signal pilin, BcpA, D forms an amide bond between C-terminal threonine amino group lysine within YPKN motif another BcpA subunit. Pilus assembly terminates upon A signal, resulting in covalent cell wall cross-bridge. Here, we show that IPNTG BcpB, is cleaved by but not A. The BcpB amide-linked to...
The secondary cell wall polysaccharide (SCWP) is thought to be essential for vegetative growth and surface (S)-layer assembly in Bacillus anthracis; however, the genetic determinants of its trisaccharide repeat structure are not known. Here, we report that WpaA (BAS0847) WpaB (BAS5274) share features with membrane proteins involved O-antigen lipopolysaccharide Gram-negative bacteria propose contribute SCWP B. anthracis Vegetative forms wpaA mutant displayed increased lengths chains, a...
D-Glutamate is an essential component of bacterial peptidoglycan and a building block the poly-γ-D-glutamic acid (PDGA) capsule Bacillus anthracis, causative agent anthrax. Earlier work suggested that two glutamate racemases, encoded by racE1 racE2, are each for growth B. supplying D-glutamic synthesis PDGA capsule. could not explain, however, why enzymes catalyze same reaction may be needed growth. Here, we report deletion or racE2 did prevent anthracis Sterne (pXO1(+) pXO2(-)),...
Bacillus cereus strains harboring a pXO1-like virulence plasmid cause respiratory anthrax-like disease in humans, particularly welders. We developed mouse models for intraperitoneal as well aerosol challenge with spores of B. G9241, pBCXO1 and pBC218 plasmids. Compared to wild-type deletion the pBCXO1-carried protective antigen gene (pagA1) were severely attenuated, whereas pBC218-carried homologue (pagA2) not. Anthrax vaccine adsorbed (AVA) immunization raised antibodies that bound...